Cytogenetic study of Argentinian Creole Patagonic biotype cattle

Twenty five animals belonging to the Argentinian Creole breed cattle Patagonic biotype, biotype originated only by the natural selection by the reasons of geographical isolation, were cytogenetically studied. The analized animals were not carrier of chromosomic alterations involved on the fertility, as the 1/29 robertsonian translocation. That would explain the high fertility observed in these animals in relation with other American Creole breeds in which was observed the presence of this translocation and others.Se han analizado citogenéticamente 25 animales bovinos pertenecientes a la raza Criolla Argentina biotipo Patagónico, raza sobre la que no se ha actuado de forma selectiva sino que sólo ha actuado la selección natural por razones de aislamiento. Se ha observado en nuestro trabajo que no son portadores de alteraciones cromosómicas que puedan incidir sobre la fertilidad de los mismos, como la translocación robertsoniana 1/29, lo que explicaría la alta fertilidad que poseen en comparación con otras razas Criollas americanas en las que sí se han encontrado animales portadores de esta translocación y de otras alteraciones cromosómicas

SEDOPTICA newsletter: noticias de SEDOPTICA

El boletín de noticias de SEDOPTICA recoge la actividad de la sociedad y sus miembros en el tercer trimestre del año 2019.Peer ReviewedPostprint (published version

SEDOPTICA newsletter: noticias de SEDOPTICA

Primer boletín de SEDOPTICA del año 2019 que recoge la intensa actividad de la sociedad y sus miembros en el primer trimestre.Peer ReviewedPostprint (published version

SEDOPTICA newsletter: noticias de SEDOPTICA

El boletín de noticias de SEDOPTICA recoge la actividad de la sociedad y de sus miembros en el cuarto y último trimestre del año 2019.Peer ReviewedPostprint (published version

Influence of sperm fertilizing concentration, sperm selection method and sperm capacitation procedure on the incidence of chromosomal numeric abnormalities in in vitro fertilized early bovine embryos

The occurrence of numerical chromosomal aberrations, widely described as a major cause of mortality in in vitro-produced (IVP) embryos, has been linked to several factors. In the present study we investigated the effect of sperm fertilising concentration and semen handling (sperm selection and capacitation) before IVF on the rate of numerical chromosomal abnormalities in bovine embryos. In all, 466 IVP cattle embryos were karyotyped throughout three sequential experiments, analysing the effects of sperm fertilising concentration (0.1, 1.0 or 10×106 spermatozoa mL-1), selection method (unselected or Percoll-selected spermatozoa) and capacitation medium (bovine serum albumin (BSA), heparin or their combination). The percentage of normal (diploid) and aberrant (haploid, polyploid or aneuploid) embryos was noted in each experiment. The rate of numerical chromosomal abnormalities was mainly affected by sperm fertilising concentration (P0.05). Finally, aneuploidy rates were not affected during the experiments (P>0.05), which suggests that they are not related to sperm-related factors. On the basis of these results, we conclude that sperm fertilising concentration is the 'paternal' key factor that affects the rate of numerical chromosomal abnormalities in IVP bovine embryos. By making small adjustments to fertilising protocols, the rate of cytogenetically aberrant embryos can be markedly reduce