691 research outputs found
v-SNARE transmembrane domains function as catalysts for vesicle fusion.
Vesicle fusion is mediated by an assembly of SNARE proteins between opposing membranes, but it is unknown whether transmembrane domains (TMDs) of SNARE proteins serve mechanistic functions that go beyond passive anchoring of the force-generating SNAREpin to the fusing membranes. Here, we show that conformational flexibility of synaptobrevin-2 TMD is essential for efficient Ca(2+)-triggered exocytosis and actively promotes membrane fusion as well as fusion pore expansion. Specifically, the introduction of helix-stabilizing leucine residues within the TMD region spanning the vesicle's outer leaflet strongly impairs exocytosis and decelerates fusion pore dilation. In contrast, increasing the number of helix-destabilizing, ß-branched valine or isoleucine residues within the TMD restores normal secretion but accelerates fusion pore expansion beyond the rate found for the wildtype protein. These observations provide evidence that the synaptobrevin-2 TMD catalyzes the fusion process by its structural flexibility, actively setting the pace of fusion pore expansion
Science verification of the new FlashCam-based camera in the 28m telescope of H.E.S.S
In October 2019 the central 28m telescope of the H.E.S.S. experiment has beenupgraded with a new camera. The camera is based on the FlashCam design whichhas been developed in view of a possible future implementation in themedium-sized telescopes of the Cherenkov Telescope Array (CTA). We report hereon the results of the science verification program that has been performedafter commissioning of the new camera, to show that the camera and softwarepipelines are working up to expectations.<br
Search for non-relativistic Magnetic Monopoles with IceCube
The IceCube Neutrino Observatory is a large Cherenkov detector instrumenting
of Antarctic ice. The detector can be used to search for
signatures of particle physics beyond the Standard Model. Here, we describe the
search for non-relativistic, magnetic monopoles as remnants of the GUT (Grand
Unified Theory) era shortly after the Big Bang. These monopoles may catalyze
the decay of nucleons via the Rubakov-Callan effect with a cross section
suggested to be in the range of to
. In IceCube, the Cherenkov light from nucleon decays
along the monopole trajectory would produce a characteristic hit pattern. This
paper presents the results of an analysis of first data taken from May 2011
until May 2012 with a dedicated slow-particle trigger for DeepCore, a
subdetector of IceCube. A second analysis provides better sensitivity for the
brightest non-relativistic monopoles using data taken from May 2009 until May
2010. In both analyses no monopole signal was observed. For catalysis cross
sections of the flux of non-relativistic
GUT monopoles is constrained up to a level of at a 90% confidence level,
which is three orders of magnitude below the Parker bound. The limits assume a
dominant decay of the proton into a positron and a neutral pion. These results
improve the current best experimental limits by one to two orders of magnitude,
for a wide range of assumed speeds and catalysis cross sections.Comment: 20 pages, 20 figure
Lateral Distribution of Muons in IceCube Cosmic Ray Events
In cosmic ray air showers, the muon lateral separation from the center of the
shower is a measure of the transverse momentum that the muon parent acquired in
the cosmic ray interaction. IceCube has observed cosmic ray interactions that
produce muons laterally separated by up to 400 m from the shower core, a factor
of 6 larger distance than previous measurements. These muons originate in high
pT (> 2 GeV/c) interactions from the incident cosmic ray, or high-energy
secondary interactions. The separation distribution shows a transition to a
power law at large values, indicating the presence of a hard pT component that
can be described by perturbative quantum chromodynamics. However, the rates and
the zenith angle distributions of these events are not well reproduced with the
cosmic ray models tested here, even those that include charm interactions. This
discrepancy may be explained by a larger fraction of kaons and charmed
particles than is currently incorporated in the simulations
Search for Relativistic Magnetic Monopoles with IceCube
We present the first results in the search for relativistic magnetic
monopoles with the IceCube detector, a subsurface neutrino telescope located in
the South Polar ice cap containing a volume of 1 km. This analysis
searches data taken on the partially completed detector during 2007 when
roughly 0.2 km of ice was instrumented. The lack of candidate events
leads to an upper limit on the flux of relativistic magnetic monopoles of
\Phi_{\mathrm{90%C.L.}}\sim 3\e{-18}\fluxunits for . This is a
factor of 4 improvement over the previous best experimental flux limits up to a
Lorentz boost below . This result is then interpreted for a
wide range of mass and kinetic energy values.Comment: 11 pages, 11 figures. v2 is minor text edits, no changes to resul
- …