Panel summary of recommendations

The following Space Station internal contamination topics were addressed: past flight experience (Skylab and Spacelab missions); present flight activities (Spacelabs and Soviet Space Station Mir); future activities (materials science and life science experiments); Space Station capabilities (PPMS, FMS, ECLSS, and U.S. Laboratory overview); manned systems/crew safety; internal contamination detection; contamination control - stowage and handling; and contamination control - waste gas processing. Space Station design assumptions are discussed. Issues and concerns are discussed as they relate to (1) policy and management, (2) subsystem design, (3) experiment design, and (4) internal contamination detection and control. The recommendations generated are summarized

Helping The Community: An Untapped Resource for Troubled Children

All too often adults have cornered the market on helping. They work within voluntary organizations and church groups to meet the needs of the community. for many this service continues within their jobs as educators and social service providers. Occasionally a place is cast aside for youth to participate. The challenge becomes how to involve troubled youth in helping others to reap the benefits popularized in the phrase It is more blessed to give than to receive. If there is strength to be derived from.giving, then let us be creative in sharing these opportunities with those who have more to gain from the benefits. Service learning is one way of allowing youth to improve their self concepts through giving of themselves to others in need

When Can You Fold a Map?

We explore the following problem: given a collection of creases on a piece of paper, each assigned a folding direction of mountain or valley, is there a flat folding by a sequence of simple folds? There are several models of simple folds; the simplest one-layer simple fold rotates a portion of paper about a crease in the paper by +-180 degrees. We first consider the analogous questions in one dimension lower -- bending a segment into a flat object -- which lead to interesting problems on strings. We develop efficient algorithms for the recognition of simply foldable 1D crease patterns, and reconstruction of a sequence of simple folds. Indeed, we prove that a 1D crease pattern is flat-foldable by any means precisely if it is by a sequence of one-layer simple folds. Next we explore simple foldability in two dimensions, and find a surprising contrast: ``map'' folding and variants are polynomial, but slight generalizations are NP-complete. Specifically, we develop a linear-time algorithm for deciding foldability of an orthogonal crease pattern on a rectangular piece of paper, and prove that it is (weakly) NP-complete to decide foldability of (1) an orthogonal crease pattern on a orthogonal piece of paper, (2) a crease pattern of axis-parallel and diagonal (45-degree) creases on a square piece of paper, and (3) crease patterns without a mountain/valley assignment.Comment: 24 pages, 19 figures. Version 3 includes several improvements thanks to referees, including formal definitions of simple folds, more figures, table summarizing results, new open problems, and additional reference

The Effects of Olfactory Enrichment on Shelter Dog Behavior

Shelter environments are stressful for dogs due to loud noises and unfamiliar surroundings. Previous research showed that exposure to some scents resulted in reductions in activity and vocalizations in shelter dogs. We investigated the effects of two calming (lavender and vetiver) and two stimulating (lemon and rosemary) essential oils on crate position and active, resting, and stress behaviors. There were 8, 5-min observations conducted each week per dog, split between baseline and scent exposure. Our analysis using the Wilcoxon Signed-Rank test found that dogs exposed to lavender (n = 13), lemon (n = 10), rosemary (n = 13), and vetiver (n = 12) did not show a significant difference in crate position or amount of time they engaged in stress, resting, or active behaviors compared to baseline. The lack of behavioral improvement indicates that scent enrichment alone may not be enough to have a significant effect on shelter dog behavior

Larval therapy for leg ulcers (VenUS II) : randomised controlled trial

Objective To compare the clinical effectiveness of larval therapy with a standard debridement technique (hydrogel) for sloughy or necrotic leg ulcers. Design Pragmatic, three armed randomised controlled trial. Setting Community nurse led services, hospital wards, and hospital outpatient leg ulcer clinics in urban and rural settings, United Kingdom. Participants 267 patients with at least one venous or mixed venous and arterial ulcer with at least 25% coverage of slough or necrotic tissue, and an ankle brachial pressure index of 0.6 or more. Interventions Loose larvae, bagged larvae, and hydrogel. Main outcome measures The primary outcome was time to healing of the largest eligible ulcer. Secondary outcomes were time to debridement, health related quality of life (SF-12), bacterial load, presence of meticillin resistant Staphylococcus aureus, adverse events, and ulcer related pain (visual analogue scale, from 0 mm for no pain to 150 mm for worst pain imaginable). Results Time to healing was not significantly different between the loose or bagged larvae group and the hydrogel group (hazard ratio for healing using larvae v hydrogel 1.13, 95% confidence interval 0.76 to 1.68; P=0.54). Larval therapy significantly reduced the time to debridement (2.31, 1.65 to 3.2; P<0.001). Health related quality of life and change in bacterial load over time were not significantly different between the groups. 6.7% of participants had MRSA at baseline. No difference was found between larval therapy and hydrogel in their ability to eradicate MRSA by the end of the debridement phase (75% (9/12) v 50% (3/6); P=0.34), although this comparison was underpowered. Mean ulcer related pain scores were higher in either larvae group compared with hydrogel (mean difference in pain score: loose larvae v hydrogel 46.74 (95% confidence interval 32.44 to 61.04), P<0.001; bagged larvae v hydrogel 38.58 (23.46 to 53.70), P<0.001). Conclusions Larval therapy did not improve the rate of healing of sloughy or necrotic leg ulcers or reduce bacterial load compared with hydrogel but did significantly reduce the time to debridement and increase ulcer pain. Trial registration Current Controlled Trials ISRCTN55114812 and National Research Register N0484123692

Dynamic subcellular localization of isoforms of the folate pathway enzyme serine hydroxymethyltransferase (SHMT) through the erythrocytic cycle of Plasmodium falciparum

<p>Abstract</p> <p>Background</p> <p>The folate pathway enzyme serine hydroxymethyltransferase (SHMT) converts serine to glycine and 5,10-methylenetetrahydrofolate and is essential for the acquisition of one-carbon units for subsequent transfer reactions. 5,10-methylenetetrahydrofolate is used by thymidylate synthase to convert dUMP to dTMP for DNA synthesis. In <it>Plasmodium falciparum </it>an enzymatically functional SHMT (PfSHMTc) and a related, apparently inactive isoform (PfSHMTm) are found, encoded by different genes. Here, patterns of localization of the two isoforms during the parasite erythrocytic cycle are investigated.</p> <p>Methods</p> <p>Polyclonal antibodies were raised to PfSHMTc and PfSHMTm, and, together with specific markers for the mitochondrion and apicoplast, were employed in quantitative confocal fluorescence microscopy of blood-stage parasites.</p> <p>Results</p> <p>As well as the expected cytoplasmic occupancy of PfSHMTc during all stages, localization into the mitochondrion and apicoplast occurred in a stage-specific manner. Although early trophozoites lacked visible organellar PfSHMTc, a significant percentage of parasites showed such fluorescence during the mid-to-late trophozoite and schizont stages. In the case of the mitochondrion, the majority of parasites in these stages at any given time showed no marked PfSHMTc fluorescence, suggesting that its occupancy of this organelle is of limited duration. PfSHMTm showed a distinctly more pronounced mitochondrial location through most of the erythrocytic cycle and GFP-tagging of its N-terminal region confirmed the predicted presence of a mitochondrial signal sequence. Within the apicoplast, a majority of mitotic schizonts showed a marked concentration of PfSHMTc, whose localization in this organelle was less restricted than for the mitochondrion and persisted from the late trophozoite to the post-mitotic stages. PfSHMTm showed a broadly similar distribution across the cycle, but with a distinctive punctate accumulation towards the ends of elongating apicoplasts. In very late post-mitotic schizonts, both PfSHMTc and PfSHMTm were concentrated in the central region of the parasite that becomes the residual body on erythrocyte lysis and merozoite release.</p> <p>Conclusions</p> <p>Both PfSHMTc and PfSHMTm show dynamic, stage-dependent localization among the different compartments of the parasite and sequence analysis suggests they may also reversibly associate with each other, a factor that may be critical to folate cofactor function, given the apparent lack of enzymic activity of PfSHMTm.</p

Picture-hanging puzzles

We show how to hang a picture by wrapping rope around n nails, making a polynomial number of twists, such that the picture falls whenever any k out of the n nails get removed, and the picture remains hanging when fewer than k nails get removed. This construction makes for some fun mathematical magic performances. More generally, we characterize the possible Boolean functions characterizing when the picture falls in terms of which nails get removed as all monotone Boolean functions. This construction requires an exponential number of twists in the worst case, but exponential complexity is almost always necessary for general functions.National Science Foundation (U.S.) (NSF grant CCF-1018388

Picture-Hanging Puzzles

We show how to hang a picture by wrapping rope around n nails, making a polynomial number of twists, such that the picture falls whenever any k out of the n nails get removed, and the picture remains hanging when fewer than k nails get removed. This construction makes for some fun mathematical magic performances. More generally, we characterize the possible Boolean functions characterizing when the picture falls in terms of which nails get removed as all monotone Boolean functions. This construction requires an exponential number of twists in the worst case, but exponential complexity is almost always necessary for general functions.Comment: 18 pages, 8 figures, 11 puzzles. Journal version of FUN 2012 pape