Hypoxic effects on RNA synthesis and RNA polymerase activity in the isolated rat heart.

Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1981 .M224. Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.H.K.)--University of Windsor (Canada), 1981

A comparison of hydroponic and soil-based screening methods to identify salt tolerance in the field in barley

Success in breeding crops for yield and other quantitative traits depends on the use of methods to evaluate genotypes accurately under field conditions. Although many screening criteria have been suggested to distinguish between genotypes for their salt tolerance under controlled environmental conditions, there is a need to test these criteria in the field. In this study, the salt tolerance, ion concentrations, and accumulation of compatible solutes of genotypes of barley with a range of putative salt tolerance were investigated using three growing conditions (hydroponics, soil in pots, and natural saline field). Initially, 60 genotypes of barley were screened for their salt tolerance and uptake of Na+, Cl–, and K+ at 150 mM NaCl and, based on this, a subset of 15 genotypes was selected for testing in pots and in the field. Expression of salt tolerance in saline solution culture was not a reliable indicator of the differences in salt tolerance between barley plants that were evident in saline soil-based comparisons. Significant correlations were observed in the rankings of genotypes on the basis of their grain yield production at a moderately saline field site and their relative shoot growth in pots at ECe 7.2 [Spearman’s rank correlation (rs)=0.79] and ECe 15.3 (rs=0.82) and the crucial parameter of leaf Na+ (rs=0.72) and Cl– (rs=0.82) concentrations at ECe 7.2 dS m−1. This work has established screening procedures that correlated well with grain yield at sites with moderate levels of soil salinity. This study also showed that both salt exclusion and osmotic tolerance are involved in salt tolerance and that the relative importance of these traits may differ with the severity of the salt stress. In soil, ion exclusion tended to be more important at low to moderate levels of stress but osmotic stress became more important at higher stress levels. Salt exclusion coupled with a synthesis of organic solutes were shown to be important components of salt tolerance in the tolerant genotypes and further field tests of these plants under stress conditions will help to verify their potential utility in crop-improvement programmes

“Un Geste Suffit”? Unpacking the Inconvenient Truths about Al Gore’s Celebrity Activism

The present work interrogates Al Gore’s persona as a climate change activist with reference to a process we describe by the neologism “personification”: the act of constructing/presenting a public persona in order to cultivate impressions that enable public figures to consolidate authoritative reputations. The formation of such a commanding persona requires the circulation of three forms of symbolic capital: cultural, celebrity, and reputational capital as well as the performance of overtly theatrical strategies calculated to establish an empathetic relationship between performer and audience. In 2006, former Vice President Al Gore released the award-winning documentary, An Inconvenient Truth. This film subsequently functioned as a catalyst for various forms of climate change activism. This paper unpacks the contradictory ideological and cultural work performed by the film with reference to what we describe as un geste suffit; that is, those gestures that merely perform individual agency in order to connect the formulation of personal ethical identity to communal forms of political activism like environmentalism. Through a close reading of the presentation of Gore’s persona in the film itself, and research which tracks the reception and political efficacy of the film, the paper contributes to the growing body of scholarship on the politics of environmental/scientific communication. Finally, we locate our reading of Gore’s film within a contemporary context by making reference to the way An Inconvenient Truth is both resistant to and complicit with the environmental policies of the Trump administration

Field Guide to Big Bone Lick, Kentucky: Birthplace of American Vertebrate Paleontology

Big Bone Lick is the birthplace of vertebrate paleontology in the Western Hemisphere and has a long and celebrated history in the exploration of the American colonial frontier and of the early United States. Notable European scientists of the 18th century such as Buffon, Cuvier, and Hunter discussed the fossils found there. Prominent Americans of the time, such as Boone, Washington, Franklin, and Jefferson are also part of the site’s history. It is the type locality for several extinct late Pleistocene megafaunal mammals, most notably the iconic American Mastodon, who were attracted to the area by salt licks dictated by the local geology. The valley of Big Bone Creek was unglaciated during the Wisconsinan advance and numerous saline springs well up through fractured bedrock of the Cincinnati Arch, providing essential minerals for the physiology of mammalian herbivores. The fossil remains at Big Bone Lick are an attritional assemblage, apparently including those that are the result of Native American predation. Archaeological remains from all local Native American cultural periods have also been found at the lick. The site is perhaps most notable in the history of science for its role in the development of comparative morphology and the establishment of the concept of extinction. This special publication reflects research and scholarship produced in conjunction with the April 2022 joint North-Central and Southeastern section meeting of the Geological Society of America. As the authors are not Kentucky Geological Survey staff, the work described herein is not a product of KGS scholarship or explicitly reflective of KGS views. Additionally, cited historical documents included in this publication may include biased language or views that misrepresent indigenous cultures.https://uknowledge.uky.edu/kgs_sp/1000/thumbnail.jp

Implementing the threshold learning outcomes for agriculture at university

The national Learning and Teaching Academics Standards statement for agriculture (AgLTAS) describes the nature and extent of the discipline and threshold learning outcomes (TLOs) that define what a graduate should know and understand and what core skills they should have at graduation. The AgLTAS statement has been endorsed by the Australian Council of Deans of Agriculture and can be used to communicate to current and future students the minimum standards of their degree, as well as be used to inform curriculum design. While the AgLTAS document provides explanatory notes to assist educators to further understand the intent of the TLOs there are no exemplars on how the AgLTAS standards can be implemented. We will present two case studies of how academics at the University of Tasmania and the University of Adelaide used the AgLTAS to map their respective agriculture curricula. An online Curriculum Mapping Tool (CMT) was used to evaluate the alignment between the curriculum and the TLOs and to identify gaps in the curriculum where improvement may be required. Workshop participants completed pre- and post-workshop questionnaires to evaluate the benefits of mapping the curriculum for the respective degrees against the AgLTAS TLOs. The pre-workshop questionnaire collected demographic data about the participants and their knowledge about the agriculture TLOs, degree and the Australian Qualifications Framework. The post-workshop questionnaire established perceived changes in each participant’s awareness, knowledge, connection with the teaching team and curriculum. It was also used to inform the development of the explanatory notes section in the AgLTAS statement. In addition, four units from each University were chosen randomly for external benchmarking. The output from the CMT demonstrated that the degrees met or in some instances exceeded the graduate level TLOs for agriculture. Determining the proficiency level for a TLO can be an issue, as to the difference between explicit learning outcomes that might be expected at introductory level, but which at advanced levels are regarded as implicit. External reviewers examined the curricula of courses at the two Universities for alignment to the TLOs to identify and enable correction for academic bias in internally mapping units. The external evaluation aligned well with the results from the CMT. A combination of external review and curriculum mapping workshops involving the entire teaching team is however still recommended when mapping degrees to TLOs. The process of curriculum mapping was shown to provide most academics with a more holistic view of how the degree meets the AgLTAS and has the potential to drive innovation in assessment design. Curriculum mapping also assisted academics by reinforcing their understanding of constructive alignment between assessment and learning outcomes at the unit, course and the discipline threshold standards. We will also provide commentary on what we believe is the next steps and implications of the AgLTAS for curriculum development, industry engagement and graduate employability in the agriculture discipline

Clostridium difficile in Retail Meat Products, USA, 2007

To determine the presence of Clostridium difficile, we sampled cooked and uncooked meat products sold in Tucson, Arizona. Forty-two percent contained toxigenic C. difficile strains (either ribotype 078/toxinotype V [73%] or 027/toxinotype III [NAP1 or NAP1-related; 27%]). These findings indicate that food products may play a role in interspecies C. difficile transmission

Behavioural responses of western gray whales to a 4-D seismic survey off northeastern Sakhalin Island, Russia

A seismic survey was conducted off the northeastern coast of Sakhalin Island, Russia in 2010. The survey area was adjacent to the only known near-shore feeding ground of the Critically Endangered population of western gray whales Eschrichtius robustus in the western Pacific south of the Aleutian Islands. This study examined the effectiveness of efforts to minimize the behavioural responses of the whales to vessel proximity and sound during the survey. Two shore-based behavioural observation teams monitored whale movements and respirations pre-, during and post-seismic survey. Theodolite tracking and focal-animal follow methods were used to collect behavioural data. Mixed linear models were used to examine deviations from 'normal' patterns in 10 movement and 7 respiration response variables in relation to vessel proximity, vessel/ whale relative orientations and 8 received sound metrics to examine if seismic survey sound and/or vessel activity influenced the whales' behaviour. Behavioural state and water depth were the best 'natural' predictors of whale movements and respiration. After considering natural variation, none of the response variables were significantly associated with seismic survey or vessel sounds. A whale's distance from shore and its orientation relative to the closest vessel were found to be significantly influenced by vessel proximity, which suggested some non-sound related disturbance. The lack of evidence that the whales responded to seismic survey sound and vessel traffic by changing either their movement or respiration patterns could indicate that the current mitigation strategy is effective. However, power analyses suggest that our sample sizes were too small to detect subtle to moderate changes in gray whale behaviour

An autonomous, in situ light-dark bottle device for determining community respiration and net community production

Author Posting. © The Author(s), 2018. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Limnology and Oceanography-Methods 16 (2018): 323-338, doi:10.1002/lom3.10247.We describe a new, autonomous, incubation-based instrument that is deployed in situ to determine rates of gross community respiration and net community production in marine and aquatic ecosystems. During deployments at a coastal pier and in the open ocean, the PHORCYS (PHOtosynthesis and Respiration Comparison-Yielding System) captured dissolved oxygen fluxes over hourly timescales that were missed by traditional methods. The instrument uses fluorescence-quenching optodes fitted into separate light and dark chambers; these are opened and closed with piston-like actuators, allowing the instrument to make multiple, independent rate estimates in the course of each deployment. Consistent with other studies in which methods purporting to measure the same metabolic processes have yielded divergent results, respiration rate estimates from the PHORCYS were systematically higher than those calculated for the same waters using a traditional two-point Winkler titration technique. However, PHORCYS estimates of gross respiration agreed generally with separate incubations in bottles fitted with optode sensor spots. An Appendix describes a new method for estimating uncertainties in metabolic rates calculated from continuous dissolved oxygen data. Multiple successful, unattended deployments of the PHORCYS represent a small step toward fully autonomous observations of community metabolism. Yet the persistence of unexplained disagreements among aquatic metabolic rate estimates — such as those we observed between rates calculated with the PHORCYS and two existing, widely-accepted bottle-based methods — suggests that a new community intercalibration effort is warranted to address lingering sources of error in these critical measurements.This research was supported by the U.S. National Science Foundation (awards OCE-1155438 to B.A.S.V.M., J.R.V., and R.G.K., and OCE- 1059884 to B.A.S.V.M.), the Woods Hole Oceanographic Institution through a Cecil and Ida Green Foundation Innovative Technology Award and an Interdisciplinary Science Award, and a U.S. Environmental Protection Agency (EPA) STAR Graduate Fellowship to J.R.C. under Fellowship Assistance Agreement no. FP-91744301-0