RelA/NF-kappaB recruitment on the bax gene promoter antagonizes p73-dependent apoptosis in costimulated T cells

The balance between antiapoptotic and proapoptotic proteins of the Bcl-2 family is critical in determining the fate of T cells in response to death stimuli. Proapoptotic genes, such as bax, are generally regulated by the p53 family of transcription factors, whereas NF-kappaB subunits can activate the transcription of antiapoptotic Bcl-2 members. Here, we show that CD28 activation protects memory T cells from irradiation-induced apoptosis by both upregulating bcl-xL and inhibiting bax gene expression. We found that p73, but not p53, binds to and trans-activates the bax gene promoter in irradiated T cells. The activation of RelA/NF-kappaB subunit in CD28 costimulated T cells and its binding onto the bax gene promoter results in suppression of bax transcription and decrease in both p73 and RNA polymerase II recruitment in vivo. RelA recruitment on the bax gene promoter is also accompanied by the lost of p300 binding and the parallel appearance of histone deacetylase-1-containing complexes. These findings identify RelA/NF-kappaB as a critical regulator of T-cell survival by affecting the balance of Bcl-2 family members

“I can’t describe it and they can’t see the rain.” an interpretative phenomenological analysis of the experience of self-harm in young adults who report difficulties identifying and describing their feelings.

Self-harm is a serious and increasing public health concern. Self-harm is significantly associated with alexithymia, a trait characterised by a difficulty identifying and describing feelings. Alexithymia has been found to be a barrier to psychological treatment. This article explores the experiences of self-harm among eight young adults who reported difficulties identifying and describing their feelings. Semi-structured interviews were conducted, with the optional use of photographs as visual stimuli for discussion. Transcripts were analysed using Interpretative Phenomenological Analysis. Two themes are presented here: The Obscure Self describes participants’ struggle to grasp a coherent sense of self, and how self-harm provided a means of physically reconnecting with their bodies. Words Fail Me describes participants’ difficulties communicating their subjective experience, which increased feelings of isolation and recourse to self-harm. There were also tentative indications that participants were able to recognise their emotional experience when expressed by others. The lack of interpersonal connection, arising from difficulties understanding feelings and communicating them to others, may create or exacerbate the context for self-harm. The findings have relevance for the treatment of self-harm, given the high, but often unacknowledged, prevalence of alexithymia in clinical populations

Rainfall rate retrieval in presence of path attenuation using C-band polarimetric weather radars

Weather radar systems are very suitable tools for the monitoring of extreme rainfall events providing measurements with high spatial and temporal resolution over a wide geographical area. Nevertheless, radar rainfall retrieval at C-band is prone to several error sources, such as rain path attenuation which affects the accuracy of inversion algorithms. In this paper, the so-called rain profiling techniques (namely the surface reference method FV and the polarimetric method ZPHI) are applied to correct rain path attenuation and a new neural network algorithm is proposed to estimate the rain rate from the corrected measurements of reflectivity and differential reflectivity. A stochastic model, based on disdrometer measurements, is used to generate realistic range profiles of raindrop size distribution parameters while a T-matrix solution technique is adopted to compute the corresponding polarimetric variables. A sensitivity analysis is performed in order to evaluate the expected errors of these methods. It has been found that the ZPHI method is more reliable than FV, being less sensitive to calibration errors. Moreover, the proposed neural network algorithm has shown more accurate rain rate estimates than the corresponding parametric algorithm, especially in presence of calibration errors

Effects of mindfulness-based interventions on alexithymia: a systematic review

Question: Alexithymia has been found to be modifiable through treatment, with associated clinical benefits. Recent studies have begun to test the potential of mindfulness-based interventions to reduce alexithymia, using skills-based, group training to improve non-judgmental, present moment awareness. The objective of this review therefore was to conduct a systematic synthesis to assess the current state of knowledge about the effect of mindfulness-based interventions on alexithymia to inform clinical practice. Study Selection and Analysis: We carried out a systematic review of the literature and found four randomised controlled trials of the effect of mindfulness-based interventions on alexithymia, with a combined total of 460 participants. Findings: A random effects meta-analysis, combining study endpoint data, showed a statistically significant effect of mindfulness-based treatment on alexithymia, (Toronto Alexithymia Scale [TAS20]) compared with the control group (mean difference = -5.28, 95% CI -9.28 to -1.28, p=0.010). Subgroup analysis was conducted to investigate sources of heterogeneity (I2=52%). Heterogeneity was reduced when the meta-analysis was restricted to interventions of a similar duration (three months or less). Conclusions: Findings from our study should be replicated in further research with larger samples; however, the results indicate that mindfulness-based interventions may be an effective treatment in reducing alexithymia

Psychosocial characteristics and social networks of suicidal prisoners: towards a model of suicidal behaviour in detention

Prisoners are at increased risk of suicide. Investigation of both individual and environmental risk factors may assist in developing suicide prevention policies for prisoners and other high-risk populations. We conducted a matched case-control interview study with 60 male prisoners who had made near-lethal suicide attempts in prison (cases) and 60 male prisoners who had not (controls). We compared levels of depression, hopelessness, self-esteem, impulsivity, aggression, hostility, childhood abuse, life events (including events occurring in prison), social support, and social networks in univariate and multivariate models. A range of psychosocial factors was associated with near-lethal self-harm in prisoners. Compared with controls, cases reported higher levels of depression, hopelessness, impulsivity, and aggression, and lower levels of self-esteem and social support (all p values <0.001). Adverse life events and criminal history factors were also associated with near-lethal self-harm, especially having a prior prison spell and having been bullied in prison, both of which remained significant in multivariate analyses. The findings support a model of suicidal behaviour in prisoners that incorporates imported vulnerability factors, clinical factors, and prison experiences, and underscores their interaction. Strategies to reduce self-harm and suicide in prisoners should include attention to such factors

The relationship between self-harm and alexithymia: a systematic review and meta-analysis

Self-harm, defined for the purpose of this review as any act of self-injury without explicit suicidal intent, is an increasing public health concern, with potential long-term implications for those who engage in it. Previous research has identified a correlational relationship between self-harm and alexithymia, an emotion processing deficit characterised by difficulties identifying and describing feelings, and an externally-orientated thinking style. Through a systematic search of the literature, the current review examines the association between alexithymia and self-harm. A meta-analysis based on 23 studies found a significant, positive relationship between self-harm and alexithymia, with a medium effect size (g = 0.57, 95% CI 0.45 to 0.71). All 23 studies used the Toronto Alexithymia Scale (TAS20) to measure alexithymia. The alexithymia subcomponents difficulty identifying feelings and difficulty describing feelings were significantly associated with self-harm, but there was no significant association between self-harm and externally-orientated thinking. The effect size of the relationship was significantly larger in adolescent samples compared with adult samples and in female compared with male samples. The definition of self-harm did not affect the effect size of the relationship between alexithymia and self-harm and the results are consistent with previous meta-analyses focused more narrowly on non-suicidal self-injury and, separately, suicidal behaviours. Heterogeneity between the included studies was high. The results support an affect regulation model of self-harm, in which self-harm is used to regulate an emotional experience that is poorly understood

Hard-to-get-at data from difficult-to-access users

This paper reports on the design and development of a suite of tools to collect, analyze and visualize a diverse range of data from sufferers of mental ill-health. The aim is to allow researchers and ultimately sufferers and clinicians to better understand the ‘individual signatures’ of factors that indicate or identify episodes of ill-health. The tools have been applied as part of a study working with clients of a mental health service that demonstrates positive results con-cerning the applicability and acceptability of the approach in developing a bet-ter understanding of the factors surrounding self-harm behavior

Digital technology: coming of age?

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