Upper Airways Microbiota in Antibiotic-Naive Wheezing and Healthy Infants from the Tropics of Rural Ecuador

Background: Observations that the airway microbiome is disturbed in asthma may be confounded by the widespread use of antibiotics and inhaled steroids. We have therefore examined the oropharyngeal microbiome in early onset wheezinginfants from a rural area of tropical Ecuador where antibiotic usage is minimal and glucocorticoid usage is absent. Materials and Methods: We performed pyrosequencing of amplicons of the polymorphic bacterial 16S rRNA gene from oropharyngeal samples from 24 infants with non-infectious early onset wheezing and 24 healthy controls (average age 10.2 months). We analyzed microbial community structure and differences between cases and controls by QIIME software. Results: We obtained 76,627 high quality sequences classified into 182 operational taxonomic units (OTUs). Firmicutes was the most common and diverse phylum (71.22% of sequences) with Streptococcus being the most common genus (49.72%). Known pathogens were found significantly more often in cases of infantile wheeze compared to controls, exemplified by Haemophilus spp. (OR = 2.12, 95% Confidence Interval (CI) 1.82–2.47; P = 5.46610223) and Staphylococcus spp. (OR = 124.1, 95%CI 59.0–261.2; P = 1.876102241). Other OTUs were less common in cases than controls, notably Veillonella spp. (OR = 0.59, 95%CI = 0.56–0.62; P = 8.06610286). Discussion: The airway microbiota appeared to contain many more Streptococci than found in Western Europe and the USA. Comparisons between healthy and wheezing infants revealed a significant difference in several bacterial phylotypes that were not confounded by antibiotics or use of inhaled steroids. The increased prevalence of pathogens such as Haemophilus and Staphylococcus spp. in cases may contribute to wheezing illnesses in this age group

Quantum Quenches in Chern Insulators.

We explore the nonequilibrium response of Chern insulators. Focusing on the Haldane model, we study the dynamics induced by quantum quenches between topological and nontopological phases. A notable feature is that the Chern number, calculated for an infinite system, is unchanged under the dynamics following such a quench. However, in finite geometries, the initial and final Hamiltonians are distinguished by the presence or absence of edge modes. We study the edge excitations and describe their impact on the experimentally observable edge currents and magnetization. We show that, following a quantum quench, the edge currents relax towards new equilibrium values, and that there is light-cone spreading of the currents into the interior of the sample.This work was supported by EPSRC Grants EP/J017639/1 and EP/K030094/1. MJB thanks the EPSRC Centre for Cross-Disciplinary Approaches to Non-Equilibrium Systems (CANES) funded under grant EP/L015854/1. MJB and MDC thank the Thomas Young Center.This is the author accepted manuscript. The final version is available from APS via http://dx.doi.org/10.1103/PhysRevLett.115.23640

Hall response and edge current dynamics in Chern insulators out of equilibrium

We investigate the transport properties of Chern insulators following a quantum quench between topological and non-topological phases. Recent works have shown that this yields an excited state for which the Chern number is preserved under unitary evolution. However, this does not imply the preservation of other physical observables, as we stressed in our previous work. Here we provide an analysis of the Hall response following a quantum quench in an isolated system, with explicit results for the Haldane model. We show that the Hall conductance is no longer related to the Chern number in the post-quench state, in agreement with previous work. We also examine the dynamics of the edge currents in finite-size systems with open boundary conditions along one direction. We show that the late-time behavior is captured by a Generalized Gibbs Ensemble, after multiple traversals of the sample. We discuss the effects of generic open boundary conditions and confinement potentials.Engineering and Physical Sciences Research Council (Grant IDs: EP/J017639/1, EP/K030094/1, EP/L015854/1), Thomas Young CenterThis is the author accepted manuscript. The final version is available from the American Physical Society via http://dx.doi.org/10.1103/PhysRevB.94.15510

Frequency of cannabis and illicit opioid use among people who use drugs and report chronic pain: A longitudinal analysis.

BACKGROUND:Ecological research suggests that increased access to cannabis may facilitate reductions in opioid use and harms, and medical cannabis patients describe the substitution of opioids with cannabis for pain management. However, there is a lack of research using individual-level data to explore this question. We aimed to investigate the longitudinal association between frequency of cannabis use and illicit opioid use among people who use drugs (PWUD) experiencing chronic pain. METHODS AND FINDINGS:This study included data from people in 2 prospective cohorts of PWUD in Vancouver, Canada, who reported major or persistent pain from June 1, 2014, to December 1, 2017 (n = 1,152). We used descriptive statistics to examine reasons for cannabis use and a multivariable generalized linear mixed-effects model to estimate the relationship between daily (once or more per day) cannabis use and daily illicit opioid use. There were 424 (36.8%) women in the study, and the median age at baseline was 49.3 years (IQR 42.3-54.9). In total, 455 (40%) reported daily illicit opioid use, and 410 (36%) reported daily cannabis use during at least one 6-month follow-up period. The most commonly reported therapeutic reasons for cannabis use were pain (36%), sleep (35%), stress (31%), and nausea (30%). After adjusting for demographic characteristics, substance use, and health-related factors, daily cannabis use was associated with significantly lower odds of daily illicit opioid use (adjusted odds ratio 0.50, 95% CI 0.34-0.74, p < 0.001). Limitations of the study included self-reported measures of substance use and chronic pain, and a lack of data for cannabis preparations, dosages, and modes of administration. CONCLUSIONS:We observed an independent negative association between frequent cannabis use and frequent illicit opioid use among PWUD with chronic pain. These findings provide longitudinal observational evidence that cannabis may serve as an adjunct to or substitute for illicit opioid use among PWUD with chronic pain

Topological marker currents in Chern insulators

Topological states of matter exhibit many novel properties due to the presence of robust topological invariants such as the Chern index. These global characteristics pertain to the system as a whole and are not locally defined. However, local topological markers can distinguish between topological phases, and they can vary in space. In equilibrium, we show that the topological marker can be used to extract the critical behavior of topological phase transitions. Out of equilibrium, we show that the topological marker spreads via a flow of currents, with a bounded maximum propagation speed. We discuss the possibilities for measuring the topological marker and its flow in experiment

Emergence of interaction effects in Bose-Einstein condensation

We present a quantitative evaluation of the predictions of mean-field theory for describing a Bose-Einstein condensate in a magnetic trap by comparing directly with experimental observations. We study the release energy from ballistic expansion and the cloud density profile as a function of mean-field effects. Significant departure of the cloud shape from both the noninteracting limit and the strongly repulsive limit is observed for our parameters, consistent with theoretical prediction

Greater risk of severe COVID-19 in Black, Asian and Minority Ethnic populations is not explained by cardiometabolic, socioeconomic or behavioural factors, or by 25(OH)-vitamin D status: study of 1326 cases from the UK Biobank.

This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Public Health, following peer review. The version of record: Zahra Raisi-Estabragh, Celeste McCracken, Mae S Bethell, Jackie Cooper, Cyrus Cooper, Mark J Caulfield, Patricia B Munroe, Nicholas C Harvey, Steffen E Petersen, Greater risk of severe COVID-19 in Black, Asian and Minority Ethnic populations is not explained by cardiometabolic, socioeconomic or behavioural factors, or by 25(OH)-vitamin D status: study of 1326 cases from the UK Biobank, Journal of Public Health, fdaa095, https://doi.org/10.1093/pubmed/fdaa095 is available online at: https://doi.org/10.1093/pubmed/fdaa095BACKGROUND: We examined whether the greater severity of coronavirus disease 2019 (COVID-19) amongst men and Black, Asian and Minority Ethnic (BAME) individuals is explained by cardiometabolic, socio-economic or behavioural factors. METHODS: We studied 4510 UK Biobank participants tested for COVID-19 (positive, n = 1326). Multivariate logistic regression models including age, sex and ethnicity were used to test whether addition of (1) cardiometabolic factors [diabetes, hypertension, high cholesterol, prior myocardial infarction, smoking and body mass index (BMI)]; (2) 25(OH)-vitamin D; (3) poor diet; (4) Townsend deprivation score; (5) housing (home type, overcrowding) or (6) behavioural factors (sociability, risk taking) attenuated sex/ethnicity associations with COVID-19 status. RESULTS: There was over-representation of men and BAME ethnicities in the COVID-19 positive group. BAME individuals had, on average, poorer cardiometabolic profile, lower 25(OH)-vitamin D, greater material deprivation, and were more likely to live in larger households and in flats/apartments. Male sex, BAME ethnicity, higher BMI, higher Townsend deprivation score and household overcrowding were independently associated with significantly greater odds of COVID-19. The pattern of association was consistent for men and women; cardiometabolic, socio-demographic and behavioural factors did not attenuate sex/ethnicity associations. CONCLUSIONS: In this study, sex and ethnicity differential pattern of COVID-19 was not adequately explained by variations in cardiometabolic factors, 25(OH)-vitamin D levels or socio-economic factors. Factors which underlie ethnic differences in COVID-19 may not be easily captured, and so investigation of alternative biological and genetic susceptibilities as well as more comprehensive assessment of the complex economic, social and behavioural differences should be prioritised

Channelling of hydrothermal fluids during the accretion and evolution of the upper oceanic crust: Sr isotope evidence from ODP Hole 1256D

ODP Hole 1256D in the eastern equatorial Pacific is the first penetration of a complete section of fast spread ocean crust down to the dike-gabbro transition, and only the second borehole to sample in situ sheeted dikes after DSDP Hole 504B. Here a high spatial resolution record of whole rock and mineral strontium isotopic compositions from Site 1256 is combined with core observations and downhole wireline geophysical measurements to determine the extent of basalt-hydrothermal fluid reaction and to identify fluid pathways at different levels in the upper ocean crust.The volcanic sequence at Site 1256 is dominated by sheet and massive lava flows but the Sr isotope profile shows only limited exchange with seawater. However, the upper margins of two anomalously thick (>25 m) massive flow sequences are strongly hydrothermally altered with elevated Sr isotope ratios and appear to be conduits of lateral low-temperature off-axis fluid flow. Elsewhere in the lavas, high 87Sr/86Sr are restricted to breccia horizons. Mineralised hyaloclastic breccias in the Lava-Dike Transition are strongly altered to Mg-saponite, silica and pyrite, indicating alteration by mixed seawater and cooled hydrothermal fluids. In the Sheeted Dike Complex 87Sr/86Sr ratios are pervasively shifted towards hydrothermal fluid values (~0.705). Dike chilled margins display secondary mineral assemblages formed during both axial recharge and discharge and have higher 87Sr/86Sr than dike cores, indicating preferential fluid flow along dike margins. Localised increases in 87Sr/86Sr in the Dike-Gabbro Transition indicates the channelling of fluids along the sub-horizontal intrusive boundaries of the 25 to 50 m-thick gabbroic intrusions, with only minor increases in 87Sr/86Sr within the cores of the gabbro bodies.When compared to the pillow lava-dominated section from Hole 504B, the Sr isotope measurements from Site 1256 suggest that the extent of hydrothermal circulation in the upper ocean crust may be strongly dependent on the eruption style. Sheet and massive flow dominated lava sequences typical of fast spreading ridges may experience relatively restricted circulation, but there may be much more widespread circulation through pillow lava-dominated sections. In addition, the Hole 1256D sheeted dikes display a much greater extent of Sr-isotopic exchange compared to dikes from Hole 504B. Because seawater-derived hydrothermal fluids must transit the dikes during their evolution to black smoker-type fluids, the different Sr-isotope profiles for Holes 504B and 1256D suggest there are significant variations in mid-ocean ridge hydrothermal systems at fast and intermediate spreading ridges, which may impact geochemical cycles of elements mobilised by fluid-rock exchange at different temperatures