879 research outputs found
A20 deficiency in lung epithelial cells protects against influenza A virus infection
A20 negatively regulates multiple inflammatory signalling pathways. We here addressed the role of A20 in club cells (also known as Clara cells) of the bronchial epithelium in their response to influenza A virus infection. Club cells provide a niche for influenza virus replication, but little is known about the functions of these cells in antiviral immunity. Using airway epithelial cell-specific A20 knockout (A20(AEC-KO)) mice, we show that A20 in club cells critically controls innate immune responses upon TNF or double stranded RNA stimulation. Surprisingly, A20(AEC-KO) mice are better protected against influenza A virus challenge than their wild type littermates. This phenotype is not due to decreased viral replication. Instead host innate and adaptive immune responses and lung damage are reduced in A20(AEC-KO) mice. These attenuated responses correlate with a dampened cytotoxic T cell (CTL) response at later stages during infection, indicating that A20(AEC-KO) mice are better equipped to tolerate Influenza A virus infection. Expression of the chemokine CCL2 (also named MCP-1) is particularly suppressed in the lungs of A20(AEC-KO) mice during later stages of infection. When A20(AEC-KO) mice were treated with recombinant CCL2 the protective effect was abrogated demonstrating the crucial contribution of this chemokine to the protection of A20(AEC-KO) mice to Influenza A virus infection. Taken together, we propose a mechanism of action by which A20 expression in club cells controls inflammation and antiviral CTL responses in response to influenza virus infection
Origin and age of The Hillocks and implications for postâglacial landscape development in the upper Lake Wakatipu catchment, New Zealand
Determining and Measuring Self-Efficacy During the Student Teaching Semester
Self-efficacy is the belief that an individual is able to control the outcomes of potentially stressful situations. How teacher candidates feel about their ability to control new challenges can affect their performance in the classroom. Those with a poorer sense of self-efficacy may believe situations are out of their control. A more positive sense of perceived self-efficacy can lead to more positive outcomes. This article reports the results of a teacher self-efficacy scale administered to elementary and secondary teacher candidates at the beginning and end of their student teaching semester. Findings suggest that perceived self-efficacy among student teachers increases throughout the course of the student teaching experience
Methane emissions, feed intake, and performance of finishing beef cattle offered maize silages harvested at 4 different stages of maturity
Methane emissions, feed intake, performance, digestibility, and rumen fermentation of finishing beef cattle offered whole-crop wheat silages differing in grain content
Quantitative EEG parameters correlate with the progression of human prion diseases
BACKGROUND: Prion diseases are universally fatal and often rapidly progressive neurodegenerative diseases. EEG has long been used in the diagnosis of sporadic Creutzfeldt-Jakob disease; however, the characteristic waveforms do not occur in all types of prion diseases. Here, we re-evaluate the utility of EEG by focusing on the development of biomarkers. We test whether abnormal quantitative EEG parameters can be used to measure disease progression in prion diseases or predict disease onset in healthy individuals at risk of disease. METHODS: In the National Prion Monitoring Cohort study, we did quantitative encephalography on 301 occasions in 29 healthy controls and 67 patients with prion disease. The patients had either inherited prion disease or sporadic Creutzfeldt-Jakob disease. We computed the main background frequency, the α and Ξ power and the α/Ξ power ratio, then averaged these within 5 electrode groups. These measurements were then compared among participant groups and correlated with functional and cognitive scores cross-sectionally and longitudinally. RESULTS: We found lower main background frequency, α power and α/Ξ power ratio and higher Ξ power in patients compared to control participants. The main background frequency, the power in the α band and the α/Ξ power ratio also differed in a consistent way among the patient groups. Moreover, the main background frequency and the α/Ξ power ratio correlated significantly with functional and cognitive scores. Longitudinally, change in these parameters also showed significant correlation with the change in clinical and cognitive scores. CONCLUSIONS: Our findings support the use of quantitative EEG to follow the progression of prion disease, with potential to help evaluate the treatment effects in future clinical-trials
Embracing additive manufacture: implications for foot and ankle orthosis design
<p>Abstract</p> <p>Background</p> <p>The design of foot and ankle orthoses is currently limited by the methods used to fabricate the devices, particularly in terms of geometric freedom and potential to include innovative new features. Additive manufacturing (AM) technologies, where objects are constructed via a series of sub-millimetre layers of a substrate material, may present the opportunity to overcome these limitations and allow novel devices to be produced that are highly personalised for the individual, both in terms of fit and functionality.</p> <p>Two novel devices, a foot orthosis (FO) designed to include adjustable elements to relieve pressure at the metatarsal heads, and an ankle foot orthosis (AFO) designed to have adjustable stiffness levels in the sagittal plane, were developed and fabricated using AM. The devices were then tested on a healthy participant to determine if the intended biomechanical modes of action were achieved.</p> <p>Results</p> <p>The adjustable, pressure relieving FO was found to be able to significantly reduce pressure under the targeted metatarsal heads. The AFO was shown to have distinct effects on ankle kinematics which could be varied by adjusting the stiffness level of the device.</p> <p>Conclusions</p> <p>The results presented here demonstrate the potential design freedom made available by AM, and suggest that it may allow novel personalised orthotic devices to be produced which are beyond the current state of the art.</p
The Womenâs Equality Party: Emergence, Organisation and Challenges
Women's political parties are designed to increase women's representation in politics. More than thirty have been established in Europe since 1987, yet there has been little systematic analysis of why and when they emerge, how they organize, and what challenges they face. We argue that the study of women's parties can offer insights into questions concerning inter and intra-party power relations and the relationship between social movements and political parties, whilst also contributing to broader debates around the 'big questions' of representation, gender (in)equality, and the dynamics of political inclusion and exclusion. This article explores these issues through a case study analysis of the UK's Women's Equality Party. Drawing upon original empirical research undertaken with party activists and officials, we argue that the party's impact has been constrained by wider organizational logics and an unequal party system, whilst it has so far adhered to traditional (male-dominated) patterns of party organisation
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