3,668 research outputs found

    Semiclassical approach to fidelity amplitude

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    The fidelity amplitude is a quantity of paramount importance in echo type experiments. We use semiclassical theory to study the average fidelity amplitude for quantum chaotic systems under external perturbation. We explain analytically two extreme cases: the random dynamics limit --attained approximately by strongly chaotic systems-- and the random perturbation limit, which shows a Lyapunov decay. Numerical simulations help us bridge the gap between both extreme cases.Comment: 10 pages, 9 figures. Version closest to published versio

    Trapping and Characterization of a Reaction Intermediate in Carbapenem Hydrolysis by \u3cem\u3eB. cereus\u3c/em\u3e Metallo-ÎČ-lactamase

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    Metallo-ÎČ-lactamases hydrolyze most ÎČ-lactam antibiotics. The lack of a successful inhibitor for them is related to the previous failure to characterize a reaction intermediate with a clinically useful substrate. Stopped-flow experiments together with rapid freeze−quench EPR and Raman spectroscopies were used to characterize the reaction of Co(II)−BcII with imipenem. These studies show that Co(II)−BcII is able to hydrolyze imipenem in both the mono- and dinuclear forms. In contrast to the situation met for penicillin, the species that accumulates during turnover is an enzyme−intermediate adduct in which the ÎČ-lactam bond has already been cleaved. This intermediate is a metal-bound anionic species with a novel resonant structure that is stabilized by the metal ion at the DCH or Zn2 site. This species has been characterized based on its spectroscopic features. This represents a novel, previously unforeseen intermediate that is related to the chemical nature of carbapenems, as confirmed by the finding of a similar intermediate for meropenem. Since carbapenems are the only substrates cleaved by B1, B2, and B3 lactamases, identification of this intermediate could be exploited as a first step toward the design of transition-state-based inhibitors for all three classes of metallo-ÎČ-lactamases

    Diversity and genetic structure of mangaba remnants in states of northeastern Brazil.

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    Made available in DSpace on 2018-01-26T23:42:36Z (GMT). No. of bitstreams: 1 diversityandgenetic.pdf: 774691 bytes, checksum: eb7b21b3d34f111e2d5febcace4cba74 (MD5) Previous issue date: 2016-04-0

    A declining major merger fraction with redshift in the local Universe from the largest-yet catalog of major and minor mergers in SDSS

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    It is difficult to accurately identify galaxy mergers and it is an even larger challenge to classify them by their mass ratio or merger stage. In previous work we used a suite of simulated mergers to create a classification technique that uses linear discriminant analysis (LDA) to identify major and minor mergers. Here, we apply this technique to 1.3 million galaxies from the SDSS DR16 photometric catalog and present the probability that each galaxy is a major or minor merger, splitting the classifications by merger stages (early, late, post-coalescence). We present publicly-available imaging predictor values and all of the above classifications for one of the largest-yet samples of galaxies. We measure the major and minor merger fraction (fmergf_{\mathrm{merg}}) and build a mass-complete sample of galaxies, which we bin as a function of stellar mass and redshift. For the major mergers, we find a positive slope of fmergf_{\mathrm{merg}} with stellar mass and negative slope of fmergf_{\mathrm{merg}} with redshift between stellar masses of 10.5<M∗(log M⊙)<11.610.5 < M_* (log\ M_{\odot}) < 11.6 and redshifts of 0.03<z<0.190.03 < z < 0.19. We are able to reproduce an artificial positive slope of the major merger fraction with redshift when we do not bin for mass or craft a complete sample, demonstrating the importance of mass completeness and mass binning. We determine that the positive trend of the major merger fraction with stellar mass is consistent with a hierarchical assembly scenario. The negative trend with redshift requires that an additional assembly mechanism, such as baryonic feedback, dominates in the local Universe.Comment: 29 pages, 22 figures, see figures 16 and 17 for a summary of the findings, accepted to MNRA

    Optimization and validation of a new microbial inhibition test for the detection of antimicrobial residues in living animals intended for human consumption

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    Even though antibiotics are necessary in livestock production, they can be harmful not only due to their toxicity, but also in view of their contribution to the emergence of antimicrobial resistance. Screening tests based on microbial growth inhibition appeared to be useful tools to prevent its entry into the food chain. They have nevertheless been traditionally carried out post mortem, leading to great economical loss and harm to the environment in case a positive sample is found. Hence, the objective was to evaluate the use of a screening test as an ante mortem alternative for the detection of antibiotic residues in meat: thus, Explorer¼-Blood test was optimized and validated. After adapting the procedure for matrix preparation, the assay parameters were assessed from 344 antibiotic-free blood serum samples. Limits of Detection (LoDs) were defined by spiking blood serum with several of the most common antimicrobials used in veterinary practice. LoDs were similar to those obtained for meat and were at or below the maximum residue limits set by EU legislation for muscle. Analyses of in vivo injected samples, previously characterized by LC-MS/MS, demonstrated the method’s accuracy and proved that Explorer¼-Blood can be considered a suitable alternative to conventional post mortem screening methods
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