1,302 research outputs found
Genetic structure of pike (Esox lucius) reveals a complex and previously unrecognized colonization history of Ireland
Aim: We investigated genetic variation of Irish pike populations and their relationship with European outgroups, in order to elucidate the origin of this species to the island, which is largely assumed to have occurred as a human-mediated introduction over the past few hundred years. We aimed thereby to provide new insights into population structure to improve fisheries and biodiversity management in Irish freshwaters.
Location: Ireland, Britain and continental Europe.
Methods: A total of 752 pike (Esox lucius) were sampled from 15 locations around Ireland, and 9 continental European sites, and genotyped at six polymorphic microsatellite loci. Patterns and mechanisms of population genetic structure were assessed through a diverse array of methods, including Bayesian clustering, hierarchical analysis of molecular variance, and approximate Bayesian computation.
Results: Varying levels of genetic diversity and a high degree of population genetic differentiation were detected. Clear substructure within Ireland was identified, with two main groups being evident. One of the Irish populations showed high similarity with British populations. The other, more widespread, Irish strain did not group with any European population examined. Approximate Bayesian computation suggested that this widespread Irish strain is older, and may have colonized Ireland independently of humans.
Main conclusions: Population genetic substructure in Irish pike is high and comparable to the levels observed elsewhere in Europe. A comparison of evolutionary scenarios upholds the possibility that pike may have colonized Ireland in two ‘waves’, the first of which, being independent of human colonization, would represent the first evidence for natural colonization of a non-anadromous 42 freshwater fish to the island of Ireland. Although further investigations using comprehensive genomic techniques will be necessary to confirm this, the present results warrant a reappraisal of current management strategies for this species
Relapsing polychondritis in human immunodeficiency virus.
Relapsing polychondritis (RP) is an inflammatory condition that has been widely accepted as autoimmune in nature and can occur in patients with immune system dysregulation. Although RP has been well documented in patients with other autoimmune conditions, such as vasculitis, its presence in patients with human immunodeficiency virus (HIV) infection has been infrequently described. We describe a case of RP in an HIV-positive patient without other identifiable autoimmune disease
Assessing the applicability of the Revised Universal Soil Loss Equation (RUSLE) to Irish Catchments
Elevated suspended sediment concentrations in fluvial environments have important implications for system ecology and even small concentrations may have serious consequences for sensitive ecosystems or organisms, such as freshwater pearl mussels (<i>Margaritifera margaritifera</i>). Informed decision making is therefore required for land managers to understand and control soil erosion and sediment delivery to the river network. However, given that monitoring of sediment fluxes requires financial and human resources which are often limited at a national scale, sediment mobilisation and delivery models are commonly used for sediment yield estimation and management. The Revised Universal Soil Loss Equation (RUSLE) is the most widely used model for overland flow erosion and can, when combined with a sediment delivery ratio (SDR), provide reasonable sediment load estimations for a catchment. This paper presents RUSLE factors established from extant GIS and rainfall datasets that are incorporated into a flexible catchment modelling approach. We believe that this is the first time that results from a RUSLE application at a national scale are tested against measured sediment yield values available from Ireland. An initial assessment of RUSLE applied to Irish conditions indicates an overestimation of modelled sediment yield values for most of the selected catchments. Improved methods for model and SDR factors estimation are needed to account for Irish conditions and catchment characteristics. Nonetheless, validation and testing of the model in this study using observed values is an important step towards more effective sediment yield modelling tools for nationwide applications
The impact of cattle access on ecological water quality in streams: Examples from agricultural catchments within Ireland
Substituted dipyridophenazine complexes of Cr(III): synthesis, enantiomeric resolution and binding interactions with calf thymus DNA
[Cr(phen)2(X2dppz)]3+ {X = H, Me, or F} have been synthesised, characterised, and chromatographically resolved into their constituent Δ and Λ enantiomers. The DNA-binding interactions of each of the racemic complexes were investigated, with the results of linear dichroism, thermal denaturation, and emission quenching studies indicative of intercalative binding to CT-DNA with a significant electrostatic contribution. UV/Vis absorption titrations suggest strong DNA binding by each of the racemic complexes, with the methylated analogue [Cr(phen)2(Me 2dppz)]3+ exhibiting the largest equilibrium binding constant. Emission quenching and UV-Vis titrations of the enantiomers of [Cr(phen)2(dppz)]3+ imply similar binding affinities for the Δ and Λ isomers, although significant differences between the circular dichroism spectra of the enantiomers in the presence of DNA connote differences in binding orientation and/or conformation between the two
The Orbital Eccentricities of Directly Imaged Companions Using Observable-Based Priors: Implications for Population-level Distributions
The eccentricity of a sub-stellar companion is an important tracer of its
formation history. Directly imaged companions often present poorly constrained
eccentricities. A recently developed prior framework for orbit fitting called
''observable-based priors'' has the advantage of improving biases in derived
orbit parameters for objects with minimal phase coverage, which is the case for
the majority of directly imaged companions. We use observable-based priors to
fit the orbits of 21 exoplanets and brown dwarfs in an effort to obtain the
eccentricity distributions with minimized biases. We present the objects'
individual posteriors compared to their previously derived distributions,
showing in many cases a shift toward lower eccentricities. We analyze the
companions' eccentricity distribution at a population level, and compare this
to the distributions obtained with the traditional uniform priors. We fit a
Beta distribution to our posteriors using observable-based priors, obtaining
shape parameters and . This represents an approximately flat distribution of
eccentricities. The derived and parameters are consistent with
the values obtained using uniform priors, though uniform priors lead to a tail
at high eccentricities. We find that separating the population into high and
low mass companions yields different distributions depending on the
classification of intermediate mass objects. We also determine via simulation
that the minimal orbit coverage needed to give meaningful posteriors under the
assumptions made for directly imaged planets is 15% of the inferred
period of the orbit.Comment: Accepted for publication in AJ, 20 pages, 13 figure
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Spectro-electrochemical studies on [Ru(TAP)2(dppz)]2+ - insights into the mechanism of its photosensitized oxidation of oligonucleotides
[Ru(TAP)2(dppz)]2+ (TAP = 1,4,5,8-tetraazaphenanthrene; dppz = dipyrido[3,2-a:2′,3′-c]phenazine) is known to photo-oxidize guanine in DNA. Whether this oxidation proceeds by direct photoelectron transfer or by proton-coupled electron transfer is still unknown. To help distinguish between these mechanisms, spectro-electrochemical experiments have been carried out with [Ru(TAP)2(dppz)]2+ in acetonitrile. The UV–vis and mid-IR spectra obtained for the one-electron reduced product were compared to those obtained by picosecond transient absorption and time-resolved infrared experiments of [Ru(TAP)2(dppz)]2+ bound to guanine-containing DNA. An interesting feature of the singly reduced species is an electronic transition in the near-IR region (with λmax at 1970 and 2820 nm). Density functional and time-dependent density functional theory simulations of the vibrational and electronic spectra of [Ru(TAP)2(dppz)]2+, the reduced complex [Ru(TAP)2(dppz)]+, and four isomers of [Ru(TAP)(TAPH)(dppz)]2+ (a possible product of proton-coupled electron transfer) were performed. Significantly, these predict absorption bands at λ > 1900 nm (attributed to a ligand-to-metal charge-transfer transition) for [Ru(TAP)2(dppz)]+ but not for [Ru(TAP)(TAPH)(dppz)]2+. Both the UV–vis and mid-IR difference absorption spectra of the electrochemically generated singly reduced species [Ru(TAP)2(dppz)]+ agree well with the transient absorption and time-resolved infrared spectra previously determined for the transient species formed by photoexcitation of [Ru(TAP)2(dppz)]2+ intercalated in guanine-containing DNA. This suggests that the photochemical process in DNA proceeds by photoelectron transfer and not by a proton-coupled electron transfer process involving formation of [Ru(TAP)(TAPH)(dppz)]2+, as is proposed for the reaction with 5′-guanosine monophosphate. Additional infrared spectro-electrochemical measurements and density functional calculations have also been carried out on the free TAP ligand. These show that the TAP radical anion in acetonitrile also exhibits strong broad near-IR electronic absorption (λmax at 1750 and 2360 nm)
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