Association of genotypes of cows of the Kholmogory breed by beta-casein with milk productivity

The aim of the study is to identify the frequency of occurrence of various allelic variants and genotypes of beta-casein in cows of the Kholmogory breed and their relationship with dairy productivity. The tasks of the research are genotyping of cattle of the Kholmogory breed by the beta-casein locus and establishing its connection with qualitative and quantitative indicators of dairy productivity. As the objects for the research there were taken 150 cows of the 1st, 2nd and 3rd lactation. An allele-specific variant of the PCR method (AS-PCR) was used to identify A1 and A2 beta-casein. As the result it had been established that in the studied part of the herd, 23 % of animals had the A2A2 genotype, 43 % of animals had the A1A1 genotype and 34 % of animals had the A1A2 genotype. For 100 days of the first lactation, animals with A1A2 genotype showed the highest value in milk yield. Animals with A2A2 genotype for 305 days of lactation had the highest milk yield and the amount of milk protein, however, the difference was not statistically significant compared to the animals with A1A2 genotype. Genotype A1A1 has lower indicators by all the parameters studied, with a significant difference relative to genotypes A1A2 and A2A2. Thus, the study of CSN2 is a promising area of scientific research, and the results of the study of beta-casein genotypes can be used as a marker selection in improving the herds of the Kholmogory breed

Клинико-статистический анализ предикторов летальных исходов у больных туберкулезом, получающих лечение в учреждениях ФСИН России

The objective: to determine risk factors of lethal outcomes (predictors) in tuberculosis patients treated with chemotherapy regimens I, II, III during confinement in the Russian penitentiary system.Subjects and Methods: data on 6,069 patients registered for treatment in 2019 and 2020 were studied. Univariate and multivariate analyses were performed.Results. The following factors were found to have effect on mortality: age (aOR = 1.05; 95% CI 1.03-1.06), multiple localizations of tuberculosis (aOR = 11.08; 95% CI 5.12-24.20), concurrent HIV infection (aOR = 9.13; 95% CI 6.90-12.24), and positive sputum microscopy at registration (aOR = 5.18; 95% CI 3.93-6.81). The patient gender was found to have no effect (OR = 0.9; 95% CI 0.4-1.9; p = 1).Цель исследования: определить факторы риска летального исхода (предикторы) у пациентов с туберкулезом, получающих лечение по I, II, III режимам химиотерапии во время нахождения в пенитенциарных учреждениях России.Материалы и методы: изучены сведения о 6 069 записях о пациентах, зарегистрированных для лечения в 2019 и 2020 г. Проводили одномерный и многомерный анализ.Результаты. Установлено влияние на частоту летального исхода возраста (aOR = 1,05; 95% CI 1,03-1,06), множественных локализаций туберкулеза (aOR = 11,08; 95% CI 5,12-24,20), наличия сопутствующей ВИЧ-инфекции (aOR = 9,13; 95% CI 6,90-12,24), положительного результата микроскопии мокроты при регистрации (aOR = 5,18; 95% CI 3,93-6,81). Не было выявлено влияния пола пациентов (OR = 0,9; 95% CI 0,4-1,9; p = 1)

Decisional needs assessment of patients with complex care needs in primary care: a participatory systematic mixed studies review protocol.

Patients with complex care needs (PCCNs) often suffer from combinations of multiple chronic conditions, mental health problems, drug interactions and social vulnerability, which can lead to healthcare services overuse, underuse or misuse. Typically, PCCNs face interactional issues and unmet decisional needs regarding possible options in a cascade of interrelated decisions involving different stakeholders (themselves, their families, their caregivers, their healthcare practitioners). Gaps in knowledge, values clarification and social support in situations where options need to be deliberated hamper effective decision support interventions. This review aims to (1) assess decisional needs of PCCNs from the perspective of stakeholders, (2) build a taxonomy of these decisional needs and (3) prioritise decisional needs with knowledge users (clinicians, patients and managers). This review will be based on the interprofessional shared decision making (IP-SDM) model and the Ottawa Decision Support Framework. Applying a participatory research approach, we will identify potentially relevant studies through a comprehensive literature search; select relevant ones using eligibility criteria inspired from our previous scoping review on PCCNs; appraise quality using the Mixed Methods Appraisal Tool; conduct a three-step synthesis (sequential exploratory mixed methods design) to build taxonomy of key decisional needs; and integrate these results with those of a parallel PCCNs' qualitative decisional need assessment (semistructured interviews and focus group with stakeholders). This systematic review, together with the qualitative study (approved by the Centre Intégré Universitaire de Santé et Service Sociaux du Saguenay-Lac-Saint-Jean ethical committee), will produce a working taxonomy of key decisional needs (ontological contribution), to inform the subsequent user-centred design of a support tool for addressing PCCNs' decisional needs (practical contribution). We will adapt the IP-SDM model, normally dealing with a single decision, for PCCNs who experience cascade of decisions involving different stakeholders (theoretical contribution). Knowledge users will facilitate dissemination of the results in the Canadian primary care network. CRD42015020558