Erranti e impreviste

On the Italian silver screens as well as in the cities, the feminine bodies have been crossing the most various landscapes. In so doing, they have been tracing peculiar, often unpredicted trajectories, as unpredicted and forbidden their presence was, if not accompanied by a man, in the spaces of the streets. This essay aims at proposing a first glance on these walking women, crossing the steps described in feminine writings to those appearing in films, from the Thirties to the aftermath of the war.On the Italian silver screens as well as in the cities, the feminine bodies have been crossing the most various landscapes. In so doing, they have been tracing peculiar, often unpredicted trajectories, as unpredicted and forbidden their presence was, if not accompanied by a man, in the spaces of the streets. This essay aims at proposing a first glance on these walking women, crossing the steps described in feminine writings to those appearing in films, from the Thirties to the aftermath of the war

Evolutionary History of Chromosome 20

The evolutionary history of human chromosome 20 in primates was investigated using a panel of human BAC/PAC probes spaced along the chromosome. Oligonucleotide primers derived from the sequence of each human clone were used to screen horse, cat, pig, and black lemur BAC libraries to assemble, for each species, a panel of probes mapping to chromosomal loci orthologous to the loci encompassed by the human BACs. This approach facilitated marker-order comparison aimed at defining marker arrangement in primate ancestor. To this goal, we also took advantage of the mouse and rat draft sequences. The almost perfect colinearity of chromosome 20 sequence in humans and mouse could be interpreted as evidence that their form was ancestral to primates. Contrary to this view, we found that horse, macaque, and two New World monkeys share the same marker-order arrangement from which the human and mouse forms can be derived, assuming similar but distinct inversions that fully account for the small difference in marker arrangement between humans and mouse. The evolutionary history of this chromosome unveiled also two centromere repositioning events in New World monkey species

Somatic Embryogenesis in Vitis for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes

New Plant Breeding Techniques (NPBTs) protocols have been developed to produce new grape varieties with improved quantitative and qualitative characteristics. Reliable transformation protocols for grapes are based on the generation/induction of embryogenic callus cells that are then transformed. Varieties such as Italia have proven to be very recalcitrant to regeneration via somatic embryogenesis. In this work, the development of a protocol for improved production of embryogenic calluses is described. Two sterilization protocols were tested: (a) a lower active chlorine concentration for a longer time (LS); and (b) a higher chlorine concentration for a shorter time (HS), in combination with the absence or presence of citric acid in the growing substrate in the first growth media. The embryogenic calluses formation in Chardonnay, a cv. with a high embryogenic response, was significantly higher in presence of citric acid in the initial growing substrate regardless of the sterilization protocol. In Aglianico, a cv. with a lower embryogenic response, no significant differences were observed. Instead, in a recalcitrant cv. as Italia, we obtained a 13-fold increase in embryogenic calluses formation performing sterilization of flowers with the HS protocol compared to LS

Comparing galactic satellite properties in hydrodynamical and Nbody simulations

In this work, we examine the different properties of galactic satellites in hydrodynamical and pure dark matter simulations. We use three pairs of simulations (collisional and collision-less) starting from identical initial conditions. We concentrate our analysis on pairs of satellites in the hydro and Nbody runs that form from the same Lagrangian region. We look at the radial positions, mass loss as a function of time and orbital parameters of these "twin" satellites. We confirm an overall higher radial density of satellites in the hydrodynamical runs, but find that trends in the mass loss and radial position of these satellites in the inner and outer region of the parent halo differ from the pure dark matter case. In the outskirts of the halo (~70% of the virial radius) satellites experience a stronger mass loss and higher dynamical friction in pure dark matter runs. The situation is reversed in the central region of the halo, where hydrodynamical satellites have smaller apocenter distances and suffer higher mass stripping. We partially ascribe this bimodal behaviour to the delayed infall time for hydro satellites, which on average cross the virial radius of the parent halo 0.7 Gyrs after their dark matter twins. Finally, we briefly discuss the implications of the different set of satellite orbital parameters and mass loss rates in hydrodynamical simulations within the context of thin discs heating and destruction.Comment: 10 pages, 15 figures, accepted for publication on MNRA