Physical, Mechanical, and Biological Properties of PMMA-Based Composite Bone Cement Containing Silver-Doped Bioactive and Antibacterial Glass Particles with Different Particles Sizes

Abstract: In the present work, antibacterial composite bone cement was designed by introducing a bioactive and antibacterial glass into a commercial formulation. The effect of glass particles’ addition on the curing parameters of the polymeric matrix was evaluated; moreover, the influence of the glass particle size on the glass dispersion, compressive and bending strength, bioactivity, and antibacterial effect was estimated. The results evidence a delay in the polymerization kinetics of the composite cement, which nevertheless complies with the requirements of the ISO standard. Morphological characterization provides evidence of good dispersion of the glass in the polymeric matrix and its exposition on the cement surface. The different glass grain sizes do not affect the composites’ bioactivity and compressive strength, while a slight reduction in bending strength was observed for samples containing glass powders with greater dimensions. The size of the glass particles also appears to have an effect on the antibacterial properties, since the composites containing larger glass particles do not produce an inhibition halo towards the S. aureus strain. The obtained results demonstrate that, by carefully tailoring the glass amount and size, a multifunctional device for artificial joint fixing, temporary prostheses, or spinal surgery can be obtained

Functionalization of plastic parts by replication of variable pitch laser-induced periodic surface structures

Surface functionalization of plastic parts has been studied and developed for several applications. However, demand for the development of reliable and profitable manufacturing strategies is still high. Here we develop and characterize a new process chain for the versatile and cost-effective production of sub-micron textured plastic parts using laser ablation. The study includes the generation of different sub-micron structures on the surface of a mold using femtosecond laser ablation and vario-thermal micro-injection molding. The manufactured parts and their surfaces are characterized in consideration of polymer replication and wetting behavior. The results of the static contact angle measurements show that replicated Laser-Induced Periodic Surface Structures (LIPSSs) always increase the hydrophobicity of plastic parts. A maximum contact angle increase of 20% was found by optimizing the manufacturing thermal boundary conditions. The wetting behavior is linked to the transition from a Wenzel to Cassie-Baxter state, and is crucial in optimizing the injection molding cycle time

Pseudomonas protegens MP12: A plant growth-promoting endophytic bacterium with broad-spectrum antifungal activity against grapevine phytopathogens

Abstract Pseudomonas sp. MP12 was isolated from a soil sample collected in a typical warm-temperate deciduous forest near Brescia, Northern Italy. Phylogenetic analysis identified the species as Pseudomonas protegens. We evidenced in this strain the presence of the genes phlD, pltB and prnC responsible for the synthesis of the antifungal compounds 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin and pyrrolnitrin, respectively. P. protegens MP12 was also shown to produce siderophores and ammonia, yielded positive results with the indole-3-acetic acid test, and was capable of phosphate solubilization. Moreover, P. protegens MP12 exhibited inhibitory effects on in vitro mycelial growth of prominent grapevine (Vitis vinifera) phytopathogens such as Botrytis cinerea, Alternaria alternata, Aspergillus niger, Penicillium expansum and Neofusicoccum parvum. The strain showed activity even against Phaeomoniella chlamydospora and Phaeoacremonium aleophilum, which cause the devastating tracheomycosis/esca disease of grapevine trunks for which no efficacious control methods have been demonstrated so far. Furthermore, the MP12 strain manifested in vivo antifungal activity against B. cinerea on grapevine leaves. Culture-dependent and culture-independent analysis revealed the ability of P. protegens MP12 to efficiently and permanently colonize inner grapevine tissues. These results suggest that P. protegens MP12 could be worth of exploitation as an antifungal biocontrol agent for applications in viticulture

Modeling the replication of submicron-structured surfaces by micro injection molding

Abstract The replication of submicron surface structures by micro injection molding is a crucial factor in achieving advanced functionalities, such as antimicrobial resistance, in mass-produced plastic products. In this work, we investigate and model the replication quality of laser-induced periodic surface structures by micro injection molding of different bio-based polymers. A comprehensive multiscale model was developed to predict the submicron scale polymer flow, using a numerical model to analyze the polymer behavior in the mold macro cavity and determine the boundary conditions for the filling of the surface structures. The replication of the mold topography was modeled considering topographical parameters, polymer rheology and thermal behavior, and the mold surface energy, which was modified by depositing an atomic layer of alumina on the steel surface structures. The modeling approach was validated against injection molding experiments, in which the mold temperature was varied due to its well-known influence on replication. The sensitivity to polymer selection, mold surface properties, and mold temperature, was captured. A maximum error of 8% showed the accuracy of the multi-scale model

A penetration efficiency model for the optimization of solid conical microneedles’ geometry

Microneedles (MNs) are promising alternatives to pills and traditional needles as drug delivery systems due to their fast, localized, and relatively less painful administration. Filling a knowledge gap, this study investigated and optimized the most influential geometrical factors determining the penetration efficiency of MNs. The effects of height, base diameter, and tip diameter were analyzed using the finite element method, with results showing that the most influencing factor was base diameter, followed by height. Moreover, the taper angle, which is dependent on all the geometrical factors, was found to directly affect the penetration efficiency at a fixed height. An additional model was developed to relate the height and taper angle to penetration efficiency, and the results were experimentally validated by compression testing of MN array prototypes printed using two-photon photolithography. The numerical model closely predicted the experimental results, with a root mean square error of 9.35. The results of our study have the potential to aid the design of high-penetration efficiency MNs for better functionality and applicability

Investigation of the influence of vacuum venting on mould surface temperature in micro injection moulding

YesThe application of vacuum venting for the removal of air from mould cavity has been introduced in injection moulding with the intent to enhance micro/nano features replication and definition. The technique is adopted to remove air pockets trapped in the micro-features, which are out of reach for conventional venting technologies and can create considerable resistance to the melt filling flow. Nonetheless, several studies have revealed a negative effect on replication that could possibly arise from the application of vacuum venting. Although the incomplete filling of micro-scale features has often been attributed to poor venting, the limited research examining the application of vacuum venting has produced mixed results. In this work, the effect of air evacuation was experimentally investigated, monitoring mould and polymer temperature evolution during the micro injection moulding process by means of a high speed infrared camera and a sapphire window, which forms part of the mould wall. The results show that air evacuation removes a mould surface heating effect caused by rapid compression of the air ahead of the flow front and subsequent conduction of that heat into the mould surface. Hence, with the increase of the surface-to-volume ratio in micro-cavities, air evacuation has a detrimental effect on the cavity filling with polymers that are sensitive to changes of the mould temperature

Effects of surface topography on growth and osteogenic differentiation of human mesenchymal stem cells

The clinical success of an endosseous artificial implant is related to the quality of its osseointegration with the surrounding living bone. To achieve a stable anchorage, mesenchymal cells, migrating to the implant surface from the surrounding tissue, must differentiate towards mature osteoblasts rather than connective tissue cell types. It is well known that the cell response is affected by the physicochemical parameters of the biomaterial surface, such as surface energy, surface charges or chemical composition. Topography seems to be one of the most crucial physical cues for cells (1). In particular, interactions between mesenchymal stem cells (MSCs) and surfaces with specific micro and nano patterns can stimulate MSCs to produce bone mineral in vitro (2). Herein, stamps reporting different micro and nano features were fabricated in order to obtain several corresponding replicas in a short time through microinjec- tion molding. Then, the effects of the substrate topography on human bone marrowderived MSC adhesion, proliferation, and osteogenic differentiation were investigated in the absence of inductive growth factors. Collectively, our data show that both micro- and nano-structured surfaces possess osteoinductive properties. A relationship between dimensional feature of surface topography and differentiative potential was noted. On the contrary, cell adhesion and proliferation seemed to be unaffected. Further in vivo studies will be carried out to confirm the osteoinductive properties of selected surface geometries

Comparison of crystallization characteristics and mechanical properties of polypropylene processed by ultrasound and conventional micro injection molding

YesUltrasound injection molding has emerged as an alternative production route for the manufacturing of micro-scale polymeric components, where it offers significant benefits over the conventional micro-injection molding process. In this work, the effects of ultrasound melting on the mechanical and morphological properties of micro-polypropylene parts were characterized. The ultrasound injection molding process was experimentally compared to the conventional micro-injection molding process using a novel mold, which allows mounting on both machines and visualization of the melt flow for both molding processes. Direct measurements of the flow front speed and temperature distributions were performed using both conventional and thermal high-speed imaging techniques. The manufacturing of micro-tensile specimens allowed the comparison of the mechanical properties of the parts obtained with the different processes. The results indicated that the ultrasound injection molding process could be an efficient alternative to the conventional process

Maria Nallino (1908-1974) and the Birth of Arabic and Islamic studies at Ca’ Foscari

This essay, based on bibliographic and archival material, focuses on the academic figure Maria Nallino, a scholar whose voluminous body of work ranges from classicism to modernity with equal fluency and expertise. Daughter of the famous Orientalist Carlo Alfonso Nallino (1872-1938), whose work she gathered and actively promoted, her arrival at Ca’ Foscari (1962) inaugurated Arabic and Islamic studies in Venice