8 research outputs found
StressprÀvention im Jugendalter. Entwicklung eines Trainingsprogramms
Beyer A, Lohaus A. StressprĂ€vention im Jugendalter. Entwicklung eines Trainingsprogramms. In: Röhrle B, ed. PrĂ€vention und Gesundheitsförderung, Band III: Kinder und Jugendliche. Fortschritte der Gemeindepsychologie und Gesundheitsförderung. Vol 16. TĂŒbingen: DGVT-Verlag; 2007
StressprÀvention im Jugendalter. Entwicklung eines Trainingsprogramms
Beyer A, Lohaus A. StressprĂ€vention im Jugendalter. Entwicklung eines Trainingsprogramms. In: Röhrle B, ed. PrĂ€vention und Gesundheitsförderung, Band III: Kinder und Jugendliche. Fortschritte der Gemeindepsychologie und Gesundheitsförderung. Vol 16. TĂŒbingen: DGVT-Verlag; 2007
Problemlösen und Entspannung als StressbewÀltigungsstrategien im Kindesalter
Lohaus A, Klein-HeĂling J. Problemlösen und Entspannung als StressbewĂ€ltigungsstrategien im Kindesalter. In: Röhrle B, Sommer G, eds. PrĂ€vention und Gesundheitsförderung. Fortschritte der Gemeindepsychologie und Gesundheitsförderung. Vol 4. TĂŒbingen: DGVT-Verlag; 1999: 233-251
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Trypsin-Free Cultivation of 3D Mini-Tissues in an Adaptive Membrane Bioreactor
The production of large scaffold-free tissues is a key challenge in regenerative medicine. Nowadays, temperature-responsive polymers allow intact tissue harvesting without needing proteolytic enzymes. This method is limited to tissue culture plastic with limited upscaling capacity and plain process control. Here, a thermoresponsive hollow fiber membrane bioreactor is presented to produce large scaffold-free tissues. Intact tissues, rich in cell-to-cell connections and ECM, are harvested from a poly(N-vinylcaprolactam) microgel functionalized poly(ether sulfone)/poly(vinylpyrrolidone) hollow fiber membrane by a temperature shift. The harvested 3D tissues adhere in successive cultivation and exhibit high vitality for several days. The facile adsorptive coating waives the need for extensive surface treatment. The research is anticipated to be a starting point for upscaling the production of interconnected tissues enabling new opportunities in regenerative medicine, large-scale drug screening on physiological relevant tissues, and potentially opening new chances in cell-based therapies. © 2020 The Authors. Advanced Biosystems published by Wiley-VCH Gmb
TrypsinâFree Cultivation of 3D MiniâTissues in an Adaptive Membrane Bioreactor
The production of large scaffold-free tissues is a key challenge in regenerative medicine. Nowadays, temperature-responsive polymers allow intact tissue harvesting without needing proteolytic enzymes. This method is limited to tissue culture plastic with limited upscaling capacity and plain process control. Here, a thermoresponsive hollow fiber membrane bioreactor is presented to produce large scaffold-free tissues. Intact tissues, rich in cell-to-cell connections and ECM, are harvested from a poly(N-vinylcaprolactam) microgel functionalized poly(ether sulfone)/poly(vinylpyrrolidone) hollow fiber membrane by a temperature shift. The harvested 3D tissues adhere in successive cultivation and exhibit high vitality for several days. The facile adsorptive coating waives the need for extensive surface treatment. The research is anticipated to be a starting point for upscaling the production of interconnected tissues enabling new opportunities in regenerative medicine, large-scale drug screening on physiological relevant tissues, and potentially opening new chances in cell-based therapies. © 2020 The Authors. Advanced Biosystems published by Wiley-VCH Gmb