Finished Genome Sequence of Collimonas arenae Cal35.

We announce the finished genome sequence of soil forest isolate Collimonas arenae Cal35, which comprises a 5.6-Mbp chromosome and 41-kb plasmid. The Cal35 genome is the second one published for the bacterial genus Collimonas and represents the first opportunity for high-resolution comparison of genome content and synteny among collimonads

The Periodontal-Endodontic Relationship, What Do We Know?

Diagnosis and management of periodontal-endodontic lesions are often complicated by the close interrelationship between periodontal tissues and dental pulps. Communications between both biological entities may occur through the apical foramen, accessory canals or exposed dentinal tubules, allowing bi-directional spread of infection and/or inflammation. Endodontic and periodontal lesions may occur distinctly or in tandem. Infected pulps may provoke an inflammatory response in adjoining periodontal tissues, and induce tissue destruction, and likewise, periodontal infection may elicit progressive pulpal pathoses. Solely periodontal or solely endodontic lesions are often clinically recognizable as distinct pathologies. However reported pain from pulpal or periodontal tissues may be similar, especially in combined lesions in which both endodontic and periodontal infection co-exist. When combined lesions develop, signs and symptoms such as toothache, tooth mobility, increased probing pocket depths and localized swelling may develop concurrently. As such, appropriate diagnostic tests and detailed clinical examination are required to differentiate periodontal, endodontic and combined pathologies and to arrive at correct diagnoses. Successful treatment outcomes for any periodontal and/or endodontic lesion depend on correct diagnosis and timely implementation of appropriate therapies. In this chapter, available evidence on periodontal-endodontic lesions will be reviewed with classification, clinical presentations, prognoses and treatment modalities discussed collectively

Pre-feasibility study of a biogas plant for peyrelevade community

This work presents a technical and financial pre-feasibility assessment for heat generation from biogas for a retirement home in the community of Peyrelevade, France. The heat demand of the retirement home currently relies on fuel oil boiler and electric heaters. The biogas is to be produced mainly from grass silage. As result of the feedstock abundance in the community, electricity can be also generated in an 80kWe CHP unit operating in full power capacity. The electricity produced can be then sold to the national electricity utility in France. The financial analysis was carried out using RETScreenTM. An efficiency of 62.9% is achievable in this CHP (Combined heat and power) biogas plant, with a total life cycle saving/income of 0.05€ for each MJ of heat supplied to the retirement home. A company set-up has also been proposed for the development of a community based compan

Pre-feasibility study of a biogas plant for peyrelevade community

This work presents a technical and financial pre-feasibility assessment for heat generation from biogas for a retirement home in the community of Peyrelevade, France. The heat demand of the retirement home currently relies on fuel oil boiler and electric heaters. The biogas is to be produced mainly from grass silage. As result of the feedstock abundance in the community, electricity can be also generated in an 80kWe CHP unit operating in full power capacity. The electricity produced can be then sold to the national electricity utility in France. The financial analysis was carried out using RETScreenTM. An efficiency of 62.9% is achievable in this CHP (Combined heat and power) biogas plant, with a total life cycle saving/income of 0.05€ for each MJ of heat supplied to the retirement home. A company set-up has also been proposed for the development of a community based compan

hnRNP A1 interacts with the genomic and subgenomic RNA promoters of Sindbis virus and is required for the synthesis of G and SG RNA

<p>Abstract</p> <p>Background</p> <p>Sindbis virus (SV) is the prototype of alphaviruses which are a group of widely distributed human and animal pathogens. Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is an RNA-binding protein that shuttles between the nucleus and the cytoplasm. Our recent studies found that hnRNP A1 relocates from nucleus to cytoplasm in Sindbis virus (SV)-infected cells. hnRNP A1 binds to the 5' UTR of SV RNA and facilitates the viral RNA replication and translation.</p> <p>Methods</p> <p>Making use of standard molecular techniques, virology methods and an in <it>vitro </it>system developed by our lab to assess the role of hnRNP A1 in SV positive strand RNA synthesis.</p> <p>Results</p> <p>hnRNP A1 interacted with the genomic (G) and subgenomic (SG) RNA promoters. Knockdown of hnRNP A1 resulted in markedly decrease in the synthesis of G and SG RNA both in infected cells and <it>in vitro</it>.</p> <p>Conclusions</p> <p>Our study provides the first direct evidence that hnRNP A1 actively participates in viral RNA replication and is required for the synthesis of G and SG RNA.</p

The ABC transporter gene family of Caenorhabditis elegans has implications for the evolutionary dynamics of multidrug resistance in eukaryotes

BACKGROUND: Many drugs of natural origin are hydrophobic and can pass through cell membranes. Hydrophobic molecules must be susceptible to active efflux systems if they are to be maintained at lower concentrations in cells than in their environment. Multi-drug resistance (MDR), often mediated by intrinsic membrane proteins that couple energy to drug efflux, provides this function. All eukaryotic genomes encode several gene families capable of encoding MDR functions, among which the ABC transporters are the largest. The number of candidate MDR genes means that study of the drug-resistance properties of an organism cannot be effectively carried out without taking a genomic perspective. RESULTS: We have annotated sequences for all 60 ABC transporters from the Caenorhabditis elegans genome, and performed a phylogenetic analysis of these along with the 49 human, 30 yeast, and 57 fly ABC transporters currently available in GenBank. Classification according to a unified nomenclature is presented. Comparison between genomes reveals much gene duplication and loss, and surprisingly little orthology among analogous genes. Proteins capable of conferring MDR are found in several distinct subfamilies and are likely to have arisen independently multiple times. CONCLUSIONS: ABC transporter evolution fits a pattern expected from a process termed 'dynamic-coherence'. This is an unusual result for such a highly conserved gene family as this one, present in all domains of cellular life. Mechanistically, this may result from the broad substrate specificity of some ABC proteins, which both reduces selection against gene loss, and leads to the facile sorting of functions among paralogs following gene duplication

Control of Formation-Flying Multi-Element Space Interferometers with Direct Interferometer-Output Feedback

The long-baseline space interferometer concept involving formation flying of multiple spacecrafts holds great promise as future space missions for high-resolution imagery. A major challenge of obtaining high-quality interferometric synthesized images from long-baseline space interferometers is to accurately control these spacecraft and their optics payloads in the specified configuration. Our research focuses on the determination of the optical errors to achieve fine control of long-baseline space interferometers without resorting to additional sensing equipment. We present a suite of estimation tools that can effectively extract from the raw interferometric image relative x/y, piston translational and tip/tilt deviations at the exit pupil aperture. The use of these error estimates in achieving control of the interferometer elements is demonstrated using simulated as well as laboratory-collected interferometric stellar images

Realizing a Robust, Reconfigurable Active Quenching Design for Multiple Types of Single-Photon Avalanche Detectors

Most active quench circuits used for single-photon avalanche photodetectors (APDs) are designed either with discrete components which lack the flexibility of dynamically changing the control parameters, or with custom ASICs which require a long development time and high cost. As an alternative, we present a reconfigurable and robust hybrid design implemented using a System-on-Chip (SoC), which integrates both an FPGA and a microcontroller. We take advantage of the FPGA's speed and reconfiguration capabilities to vary the quench and reset parameters dynamically over a large range, thus allowing our system to operate a variety of APDs without changing the design. The microcontroller enables the remote adjustment of control parameters and calibration of APDs in the field. The ruggedized design uses components with space heritage, thus making it suitable for space-based applications in the fields of telecommunications and quantum key distribution (QKD). We demonstrate our circuit by operating a commercial APD cooled to -20{\deg}C with a deadtime of 35ns while maintaining the after-pulsing probability at close to 3%. We also showcase its versatility by operating custom-fabricated chip-scale APDs, which paves the way for automated wafer-scale characterization.Comment: 6 pages, 6 figures. arXiv admin note: substantial text overlap with arXiv:2205.0022