Father-daughter Incest: Comparison of Treated Cases to Untreated Control Subjects

Treatment of father daughter incest (FDI) survivors using the victim advocacy/child welfare approach to FDI that predominates today in the US has never been compared to results in untreated control subjects in any published report. In the present study, thirty-two survivors of FDI who received treatment based on the victim advocacy/child welfare approach to FDI were compared to 32 control subjects who did not receive treatment. No significant differences were found using analysis of variance on 21 scales and subscales. Statistical analysis of the data from all 64 of the FDI survivors showed that items reflecting a poor self-image and a history of maternal depression predicted more problematic scores on the depression scale. Those who scored higher on religiosity were less likely to be depressed. Redesigned therapy for FDI aimed, first and foremost, at healing the survivors and validated by prospective randomized trials is needed

Effects of the sex of the perpetrator on victims’ subsequent sexual behaviors and adulthood sexual orientations

Data from 2,828 female and 1,556 male adult participants from a general population convenience sample were used to measure the effect of the sex of the perpetrator on the adult sexual orientation of the victims of child sexual abuse (CSA) by adult male or female perpetrators and early incest with parents or early incest with siblings. Sexual behaviors with female perpetrators tended to promote an orientation toward sex with females and analogous behaviors with male perpetrators tended to promote an orientation toward sex with males in victims of both sexes. Many forms of incest or CSA increased the likelihood the victims would subsequently engage in behaviors with other partners or begin masturbating using adult male or female images. The subsequent early behaviors with other partners and masturbating using images of both male and female adults significantly enhanced or attenuated the conditioning resulting from interacting with the initial perpetrator. The victims’ adult sexual orientations could be statistically predicted using regression equations that took into account the sexual behaviors with both adult and underage partners and whether or not the participant had masturbated using images of male or female adults. The results were consistent with conditioning and counter-conditioning theories

Father-daughter incest: Comparison of treated cases to untreated control subjects

Treatment of father daughter incest (FDI) survivors using the victim advocacy/child welfare approach to FDI that predominates today in the US has never been compared to results in untreated control subjects in any published report. In the present study, thirty-two survivors of FDI who received treatment based on the victim advocacy/child welfare approach to FDI were compared to 32 control subjects who did not receive treatment. No significant differences were found using analysis of variance on 21 scales and subscales. Statistical analysis of the data from all 64 of the FDI survivors showed that items reflecting a poor self-image and a history of maternal depression predicted more problematic scores on the depression scale. Those who scored higher on religiosity were less likely to be depressed. Redesigned therapy for FDI aimed, first and foremost, at healing the survivors and validated by prospective randomized trials is needed

Sexual imprinting of offspring on their parents and siblings

Based on data provided anonymously by 2,611 women and 1,452 men using a computerized questionnaire, we found that 19.2% of women vs. 16% of men (p < .001) were aware of being attracted to people with physical characteristics resembling members of their nuclear families, a phenomenon called sexual imprinting. Women reported sexual imprinting on fathers (15.5%), brothers (2.5%), mothers (0.8%), and sisters (0.4%), and men reported sexual imprinting on mothers (11.5%), sisters (2.1%), fathers (1.9%), and brothers (0.5%). The likelihood of women imprinting on fathers increased when fathers were more affectionate to them and when parents were more affectionate to each other. The likelihood of women imprinting on brothers increased when they had an older brother. The likelihood of women imprinting on mothers increased when parents divorced or separated and there was a remarriage. The likelihood of men imprinting on mothers increased when mothers had a positive attitude about sex. It decreased if they had an older brother and increased if they had been the victim of child sexual abuse (CSA) by an adult female. The likelihood of men imprinting on sisters was increased if parents were affectionate to each other. Three factors predicted the likelihood of men imprinting on fathers

Effects of the sex of the perpetrator on victims’ subsequent sexual behaviors and adulthood sexual orientations

Data from 2,828 female and 1,556 male adult participants from a general population convenience sample were used to measure the effect of the sex of the perpetrator on the adult sexual orientation of the victims of child sexual abuse (CSA) by adult male or female perpetrators and early incest with parents or early incest with siblings. Sexual behaviors with female perpetrators tended to promote an orientation toward sex with females and analogous behaviors with male perpetrators tended to promote an orientation toward sex with males in victims of both sexes. Many forms of incest or CSA increased the likelihood the victims would subsequently engage in behaviors with other partners or begin masturbating using adult male or female images. The subsequent early behaviors with other partners and masturbating using images of both male and female adults significantly enhanced or attenuated the conditioning resulting from interacting with the initial perpetrator. The victims’ adult sexual orientations could be statistically predicted using regression equations that took into account the sexual behaviors with both adult and underage partners and whether or not the participant had masturbated using images of male or female adults. The results were consistent with conditioning and counter-conditioning theories

Patterns of antimicrobial drug use in veterinary primary care and specialty practice: A 6‐year multi‐institution study

Abstract Background Combatting antimicrobial resistance requires a One Health approach to antimicrobial stewardship including antimicrobial drug (AMD) use evaluation. Current veterinary AMD prescribing data are limited. Objectives To quantify companion animal AMD prescribing in primary care and specialty practice across 3 academic veterinary hospitals with particular focus on third‐generation cephalosporins, fluoroquinolones, and carbapenems. Animals Dogs and cats presented to 3 academic veterinary hospitals from 2012 to 2017. Methods In this retrospective study, AMD prescribing data from 2012 to 2017 were extracted from electronic medical records at each hospital and prescriptions classified by service type: primary care, specialty practice or Emergency/Critical Care (ECC). Hospital‐level AMD prescribing data were summarized by species, service type, AMD class, and drug. Multivariable logistic full‐factorial regression models were used to estimate hospital, year, species, and service‐type effects on AMD prescribing. Estimated marginal means and confidence intervals were plotted over time. Results The probability of systemic AMD prescribing for any indication ranged between 0.15 and 0.28 and was higher for dogs than cats (P < .05) apart from 2017 at hospital 1. Animals presented to primary care were least likely to receive AMDs (dogs 0.03‐0.15, cats 0.03‐0.18). The most commonly prescribed AMD classes were aminopenicillins/β‐lactamase inhibitors (0.02‐0.15), first‐generation cephalosporins (0.00‐0.09), fluoroquinolones (0.00‐0.04), nitroimidazoles (0.01‐0.06), and tetracyclines (0.00‐0.03). Among the highest priority classes, fluoroquinolones (dogs 0.00‐0.09, cats 0.00‐0.08) and third‐generation cephalosporins (dogs 0.00‐0.04, cats 0.00‐0.05) were most frequently prescribed. Conclusions and Clinical Importance Antimicrobial drug prescribing frequencies were comparable to previous studies. Additional stewardship efforts might focus on fluoroquinolones and third‐generation cephalosporins

Childhood and adolescent sexual behaviors predict adult sexual orientations

Anonymous retrospective data were provided by 3,443 adult participants via computer-assisted self-interview. This was the first study focused on determinants of adult sexual orientation to adjust for the effects of same-sex sibling incest. Five measures of adult sexual orientations (ASOs) provided evidence consistent with the theory that ASOs result from early sex-specific romantic attachment, conditioning caused by early sexual experiences with partners, and other experiences, such as early masturbation using human images, acting synergistically with critical period learning, and sexual imprinting. Early same-sex crushes were the most powerful predictor of ASOs, and they also increased the likelihood of engaging in early same-sex partnered and masturbation behaviors. Incestuous experiences with same-sex siblings affected the ASOs of the incest participants. And, lesbian, gay, and bisexual participants tended to have an earlier onset of puberty than heterosexual controls within sexes. However, statistical analyses showed that the incest and puberty effects were mathematically explained by the participant’s early sexual experiences with partners and other experiences such as masturbation using human images. Early same-sex crushes were predicted by nuclear family variables implying that same-sex crushes were more likely when the opposite-sex parent modeled an unsatisfactory heterosexual romantic partner

Development of a novel real-time PCR multiplex assay for detection of Streptococcus equi subspecies equi and Streptococcus equi subspecies zooepidemicus

Strangles is a contagious bacterial disease of horses caused by Streptococcus equi subspecies equi (SEE) that occurs globally. Rapid and accurate identification of infected horses is essential for controlling strangles. Because of limitations of existing PCR assays for SEE, we sought to identify novel primers and probes that enable simultaneous detection and differentiation of infection with SEE and S. equi subsp. zooepidemicus (SEZ). Comparative genomics of U.S. strains of SEE and SEZ (n = 50 each) identified SE00768 from SEE and comB from SEZ as target genes. Primers and probes for real-time PCR (rtPCR) were designed for these genes and then aligned in silico with the genomes of strains of SEE (n = 725) and SEZ (n = 343). Additionally, the sensitivity and specificity relative to microbiologic culture were compared between 85 samples submitted to an accredited veterinary medical diagnostic laboratory. The respective primer and probe sets aligned with 99.7 % (723/725) isolates of SEE and 97.1 % (333/343) of SEZ. Of 85 diagnostic samples, 20 of 21 (95.2 %) SEE and 22 of 23 SEZ (95.6 %) culture-positive samples were positive by rtPCR for SEE and SEZ, respectively. Both SEE (n = 2) and SEZ (n = 3) were identified by rtPCR among 32 culture-negative samples. Results were rtPCR-positive for both SEE and SEZ in 21 of 44 (47.7 %) samples that were culture-positive for SEE or SEZ. The primers and probe sets reported here reliably detect SEE and SEZ from Europe and the U.S., and permit detection of concurrent infection with both subspecies.Fil: Morris, Ellen Ruth A.. Texas A&M University; Estados UnidosFil: Schroeder, Megan E.. Texas A&M University; Estados UnidosFil: Ferro, Pamela J.. Texas A&M University; Estados UnidosFil: Waller, Andrew Stephen. Intervacc Ab; Suecia. Sveriges Lantbruksuniversitet (slu);Fil: McGlennon, Abigail A.. University of London; Reino UnidoFil: Bustos, Carla Paola. Universidad de Buenos Aires. Facultad de Ciencias Veterinaria. Instituto de Investigaciones En Epidemiologia Veterinaria; . Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Gressler, Leticia T.. Instituto Federal Farroupilha; BrasilFil: Wu, Jing. Texas A&M University; Estados UnidosFil: Lawhon, Sara D.. Texas A&M University; Estados UnidosFil: Boyle, Ashley G.. University of Pennsylvania; Estados UnidosFil: Lingsweiler, Sonia. Texas A&M University; Estados UnidosFil: Paul, Narayan. Texas A&M University; Estados UnidosFil: Dimitrov, Kiril. Texas A&M University; Estados UnidosFil: Swinford, Amy K.. Texas A&M University; Estados UnidosFil: Bordin, Angela I.. Texas A&M University; Estados UnidosFil: Cohen, Noah D.. Texas A&M University; Estados Unido

Enhancing the one health initiative by using whole genome sequencing to monitor antimicrobial resistance of animal pathogens: Vet-LIRN collaborative project with veterinary diagnostic laboratories in United States and Canada

Abstract Background Antimicrobial resistance (AMR) of bacterial pathogens is an emerging public health threat. This threat extends to pets as it also compromises our ability to treat their infections. Surveillance programs in the United States have traditionally focused on collecting data from food animals, foods, and people. The Veterinary Laboratory Investigation and Response Network (Vet-LIRN), a national network of 45 veterinary diagnostic laboratories, tested the antimicrobial susceptibility of clinically relevant bacterial isolates from animals, with companion animal species represented for the first time in a monitoring program. During 2017, we systematically collected and tested 1968 isolates. To identify genetic determinants associated with AMR and the potential genetic relatedness of animal and human strains, whole genome sequencing (WGS) was performed on 192 isolates: 69 Salmonella enterica (all animal sources), 63 Escherichia coli (dogs), and 60 Staphylococcus pseudintermedius (dogs). Results We found that most Salmonella isolates (46/69, 67%) had no known resistance genes. Several isolates from both food and companion animals, however, showed genetic relatedness to isolates from humans. For pathogenic E. coli, no resistance genes were identified in 60% (38/63) of the isolates. Diverse resistance patterns were observed, and one of the isolates had predicted resistance to fluoroquinolones and cephalosporins, important antibiotics in human and veterinary medicine. For S. pseudintermedius, we observed a bimodal distribution of resistance genes, with some isolates having a diverse array of resistance mechanisms, including the mecA gene (19/60, 32%). Conclusion The findings from this study highlight the critical importance of veterinary diagnostic laboratory data as part of any national antimicrobial resistance surveillance program. The finding of some highly resistant bacteria from companion animals, and the observation of isolates related to those isolated from humans demonstrates the public health significance of incorporating companion animal data into surveillance systems. Vet-LIRN will continue to build the infrastructure to collect the data necessary to perform surveillance of resistant bacteria as part of fulfilling its mission to advance human and animal health. A One Health approach to AMR surveillance programs is crucial and must include data from humans, animals, and environmental sources to be effective