Single-cell immune profiling reveals thymus-seeding populations, T cell commitment, and multilineage development in the human thymus

T cell development in the mouse thymus has been studied extensively, but less is known regarding T cell development in the human thymus. We used a combination of single-cell techniques and functional assays to perform deep immune profiling of human T cell development, focusing on the initial stages of prelineage commitment. We identified three thymus-seeding progenitor populations that also have counterparts in the bone marrow. In addition, we found that the human thymus physiologically supports the development of monocytes, dendritic cells, and NK cells, as well as limited development of B cells. These results are an important step toward monitoring and guiding regenerative therapies in patients after hematopoietic stem cell transplantation

Task-Based and Individual Differences Influence the Effect of Gesture Observation on Novel L2 Speech Sound Learning

This study sought to replicate the effect of observing pitch gesture and clarify the effect of observing representational gesture on L2 lexical tone learning and to explore the influences of individual differences in lexical and non-lexical tone perception on these effects. The results revealed that observing representational gestures facilitates lexical tone discrimination, albeit to a lesser extent than observing pitch gestures, suggesting that task difficulty may influence its effect. Moreover, they revealed that individual differences in non-speech tone perception predict discrimination of lexical tones learned by observing pitch gesture and no gesture, but not representational gesture. Together, these findings suggest that task difficulty as well as individual differences in sensitivity to non-speech sounds influence the effects of observing gesture on novel L2 speech sound learning

Observing Gesture at Learning Enhances Subsequent Phonological and Semantic Processing of L2 Words: An N400 Study

This study employed the N400 event-related potential (ERP) to investigate how observing different types of gestures at learning affects the subsequent processing of L2 Mandarin words differing in lexical tone by L1 English speakers. The effects of pitch gestures conveying lexical tones (e.g., upwards diagonal movements for rising tone), semantic gestures conveying word meanings (e.g., waving goodbye for to wave), and no gesture were compared. In a lexical tone discrimination task, larger N400s for Mandarin target words mismatching vs. matching Mandarin prime words in lexical tone were observed for words learned with pitch gesture. In a meaning discrimination task, larger N400s for English target words mismatching vs. matching Mandarin prime words in meaning were observed for words learned with pitch and semantic gesture. These findings provide the first neural evidence that observing gestures during L2 word learning enhances subsequent phonological and semantic processing of learned L2 words

Observing Gestures During L2 Word Learning Facilitates Differentiation Between Unfamiliar Speech Sounds and Word Meanings

This study investigated how observing pitch gestures conveying lexical tones and representational gestures conveying word meanings when learning L2 Mandarin words differing in lexical tone affects their subsequent semantic and phonological processing in L1 English speakers using the N400 event-related potential (ERP). Larger N400s for English target words mismatching vs. matching Mandarin prime words in meaning were observed for words learned with pitch and representational gesture, but not no gesture. Additionally, larger N400s for Mandarin target words mismatching vs. matching Mandarin prime words in lexical tone were observed for words learned with pitch gesture, but not representational or no gesture. These findings provide the first ERP evidence that observing gestures conveying phonological and semantic information during L2 word learning enhances subsequent phonological and semantic processing of learned L2 words

Cognitive processing of miscommunication in interactive listening: An evaluation of listener indecision and cognitive effort

Purpose: The purpose of the current study was to evaluate the social and cognitive underpinnings of miscommunication during an interactive listening task. Method: An eye and computer mouse-tracking visualworld paradigm was used to investigate how a listener’s cognitive effort (local and global) and decision-making processes were affected by a speaker’s use of ambiguity that led to a miscommunication. Results: Experiments 1 and 2 found that an environmental cue that made a miscommunication more or less salient impacted listener language processing effort (eye-tracking). Experiment 2 also indicated that listeners may develop different processing heuristics dependent upon the speaker’s use of ambiguity that led to a miscommunication, exerting a significant impact on cognition and decision making. We also found that perspective-taking effort and decision-making complexity metrics (computer mouse tracking) predict language processing effort, indicating that instances of miscommunication produced cognitive consequences of indecision, thinking, and cognitive pull. Conclusion: Together, these results indicate that listeners behave both reciprocally and adaptively when miscommunications occur, but the way they respond is largely dependent upon the type of ambiguity and how often it is produced by the speaker.</p

Eye See What You’re Saying: Beat Gesture Facilitates Online Resolution ofContrastive Referring Expressions in Spoken Discourse

This study investigated how beat gesture and contrastive pitchaccenting affect online contrastive reference resolution duringspoken discourse comprehension. Evidence from gazefixations indicated that beat gesture encouraged fixations totarget referents of contrastive referring expressions and thatcontrastive accenting encouraged fixations to competitorreferents of non-contrastive referring expressions. Notably,beat gesture and contrastive accenting acted independently,indicating that their effects are additive rather than interactive.Moreover, neither beat gesture nor contrastive accentingaffected an observed tendency to anticipate contrastivereferring expressions. Together, these results provide the firstevidence that beat gesture, like contrastive accenting, isinterpreted as a cue to contrast during online referenceresolution in spoken discourse comprehension