ARAquá: software para avaliação de risco ambiental de agrotóxico.

O software ARAquá foi desenvolvido para auxiliar as avaliações de riscos ambientais de agrotóxicos, considerando as possíveis contaminações de corpos d?água superficiais e subterrâneos, através da comparação de suas concentrações estimadas em cenários de uso agrícola com parâmetros de qualidade de água. O programa foi desenvolvido com o Ambiente de Desenvolvimento Integrado DELPHI 7, sendo PASCAL a linguagem de programação. O software utiliza dados referentes ao local, solo e agrotóxico para a realização de seus cálculos. Para executar o aplicativo é necessário um sistema operacional da plataforma Windows e um computador com uma configuração básica de hardware. Nesse trabalho a operacionalidade do ARAquá é exemplificada com dados de entrada pré-cadastrados, que seguem com o software.bitstream/item/35294/1/boletim6ATUAL.pd

Migration of surgical clips through a right lobectomy stump mimicking an asthmatic syndrome

The mechanical stapler is routinely used in thoracic surgery practice to attend resection of bronchus and vessels. Herein, we reported a very rare complication as the migration of a titanium surgical clip through a right lobectomy stump. One year after the procedure, the patient complained of persistent cough. A misdiagnosis of asthma was made and she treated for 6 months with bronchodilators, corticosteroid and antihistaminic without success. Thus, patient re-referred of our unit. No clinical signs of infection as fewer, productive cough, dyspnea were present. The laboratory exams were within normal value including white cells. CT scan revealed no abnormalities. Bronchoscopy demonstrated a healed upper bronchus stump without evidence of an actual, open bronchopleural fistula but with clips apparently working their way into the airway, with approximately half of the clip visible within the lumen. The side of the clips that would be open before closure by the surgeon formed the leading edge of the clips visible in the lumen. The clips were successfully removed during flexible bronchoscopy with a forceps usually used for biopsy. After the procedure, the cough disappeared. The endoscopy check after 3 months showed a normal bronchial stump without evidence of fistula

Mutations in the nuclear localization sequence of the Aristaless related homeobox; sequestration of mutant ARX with IPO13 disrupts normal subcellular distribution of the transcription factor and retards cell division

The electronic version of this article is the complete one and can be found online at: http://www.pathogeneticsjournal.com/content/3/1/1Background: Aristaless related homeobox (ARX) is a paired-type homeobox gene. ARX function is frequently affected by naturally occurring mutations. Nonsense mutations, polyalanine tract expansions and missense mutations in ARX cause a range of intellectual disability and epilepsy phenotypes with or without additional features including hand dystonia, lissencephaly, autism or dysarthria. Severe malformation phenotypes, such as X-linked lissencephaly with ambiguous genitalia (XLAG), are frequently observed in individuals with protein truncating or missense mutations clustered in the highly conserved paired-type homeodomain. Results: We have identified two novel point mutations in the R379 residue of the ARX homeodomain; c.1135C>A, p.R379S in a patient with infantile spasms and intellectual disability and c.1136G>T, p.R379L in a patient with XLAG. We investigated these and other missense mutations (R332P, R332H, R332C, T333N: associated with XLAG and Proud syndrome) predicted to affect the nuclear localisation sequences (NLS) flanking either end of the ARX homeodomain. The NLS regions are required for correct nuclear import facilitated by Importin 13 (IPO13). We demonstrate that missense mutations in either the N- or C-terminal NLS regions of the homeodomain cause significant disruption to nuclear localisation of the ARX protein in vitro. Surprisingly, none of these mutations abolished the binding of ARX to IPO13. This was confirmed by co-immunoprecipitation and immmuno fluorescence studies. Instead, tagged and endogenous IPO13 remained bound to the mutant ARX proteins, even in the RanGTP rich nuclear environment. We also identify the microtubule protein TUBA1A as a novel interacting protein for ARX and show cells expressing mutant ARX protein accumulate in mitosis, indicating normal cell division may be disrupted. Conclusions: We show that the most likely, common pathogenic mechanism of the missense mutations in NLS regions of the ARX homeodomain is inadequate accumulation and distribution of the ARX transcription factor within the nucleus due to sequestration of ARX with IPO13.Cheryl Shoubridge, May Huey Tan, Tod Fullston, Desiree Cloosterman, David Coman, George McGillivray, Grazia M Mancini, Tjitske Kleefstra and Jozef Géc

Sotaque e telejornalismo: representações de comunicadores de mídia nordestinos

RESUMO: Objetivo: descrever o conteúdo das representações sociais do comunicador de mídia nordestino acerca do seu sotaque. Métodos: a coleta de dados foi realizada em duas etapas. Na primeira, foi empregada a técnica de associação livre, na qual 50 universitários apresentaram o que pensavam diante da palavra "sotaque". Na segunda, por meio do Procedimento de Classificações Múltiplas (PCM), 25 comunicadores atuantes na Região Metropolitana do Recife classificaram as 15 palavras mais associadas pelos estudantes de duas maneiras, classificação livre e dirigida. Na classificação livre, os comunicadores agruparam as palavras segundo critérios por eles estabelecidos, enquanto que na classificação dirigida, as palavras foram classificadas de acordo com o grau de associação com o termo MEU SOTAQUE. A análise foi realizada utilizando-se métodos estatísticos multidimensionais, que permitiram construir a estruturação do campo das Representações Sociais. Resultados: três regiões delimitadas expressam a representação dos sujeitos sobre sotaque: Conceito, Identidade e Espaço. Na primeira, há seis categorias (engraçado, diferente, arrastado, matuto, preconceito, oxente), na segunda, as palavras são identidade, característica, língua, fala e cultura, próximas ao termo meu sotaque e, na terceira, região, regionalismo, nordeste e localidade. Identidade foi o termo mais relacionado ao termo MEU SOTAQUE, enquanto que os itens menos relacionados são os presentes na região conceito, exceto pelo termo oxente. Conclusão: o sotaque nordestino é tido como uma marca da identidade e cultura da região Nordeste. Os comunicadores suavizam características do falar nordestino durante o exercício profissional para se adequar ao padrão preconizado pelo mercado de trabalho sem perder a sua identidade

Models of classroom assessment for course-based research experiences

Course-based research pedagogy involves positioning students as contributors to authentic research projects as part of an engaging educational experience that promotes their learning and persistence in science. To develop a model for assessing and grading students engaged in this type of learning experience, the assessment aims and practices of a community of experienced course-based research instructors were collected and analyzed. This approach defines four aims of course-based research assessment—(1) Assessing Laboratory Work and Scientific Thinking; (2) Evaluating Mastery of Concepts, Quantitative Thinking and Skills; (3) Appraising Forms of Scientific Communication; and (4) Metacognition of Learning—along with a set of practices for each aim. These aims and practices of assessment were then integrated with previously developed models of course-based research instruction to reveal an assessment program in which instructors provide extensive feedback to support productive student engagement in research while grading those aspects of research that are necessary for the student to succeed. Assessment conducted in this way delicately balances the need to facilitate students’ ongoing research with the requirement of a final grade without undercutting the important aims of a CRE education

Diagnostic yield and safety of C-TBNA in elderly patients with lung cancer.

Conventional transbronchial needle aspiration (C-TBNA) is a minimally invasive technique used primarily in the diagnosis and staging of lung cancer. Currently lung cancer is mostly considered a disease of the elderly and the management of this disease in older patients is a growing concern. In this study we aimed to assess the diagnostic yield and safety of C-TBNA in elderly patients. A retrospective review of 88 consecutive C-TBNA procedures for nodal staging in suspected or confirmed primary lung cancer or pathological confirmation in suspected advanced lung cancer was performed. Patients were divided into less than 70 (<70yrs) or 70 and older (≥70yrs) age groups for analysis. There were no significant differences either in the diagnostic yield (69% in patients aged < 70 yrs and 74% patients aged ≥ 70 yrs (p=0.5) nor in the complication rate (respectively 8.8% in patients aged < 70 yrs and 6.9% in patients aged ≥ 70 yrs (p=0.7 ) between the two age groups. Reported complications were minor bleeding and poor tolerance; no major complications were observed. Based on our experience, C-TBNA represents a useful and safe alternative procedure for the diagnosis and staging of lung cancer in elderly patients