Platelet lysate maintains chondrogenic potential and promotes cartilage regeneration

cartilage. We report the biological effect of the platelet lysate (PL), a PRP derivative, on primary human articular chondrocytes (HAC) cultured under both physiological and inflammatory condition. Added to the culture medium, PL induced a strong mitogenic response in the chondrocytes. The in vitro expanded cell population maintained a chondrogenic re-­‐differentiation potential as revealed by micromass culture in vitro as well as in vivo as demonstrated by ectopic cartilage formation in nude mice. Furthermore, in chondrocytes cultured in the presence of the pro-­‐inflammatory cytokine IL-­‐1α, the PL induced a drastic enhancement of the synthesis of the cytokines IL-­‐6 and IL-­‐8 and of NGAL, a lipocalin expressed in cells of the chondrogenic lineage. These events were controlled by the p38 MAP kinase and NF-­‐κΒ pathways. The pro-­‐inflammatory effect of the PL was a transient phenomenon. In fact, after an initial up regulation, we observed a significant reduction of the NF-­‐κΒ activity together with the repression of the inflammatory enzyme ciclooxygenase-­‐2 (COX-­‐2). Moreover, the medium of chondrocytes cultured in the contemporary presence of PL and IL-­‐1α, showed a significant enhancement of the chemoattractant activity versus untreated chondrocytes. On the whole, our findings support the concept that the platelet products have a direct beneficial effect on articular chondrocytes and at the same time could drive in sequence a trans

Radioisotopic purity and imaging properties of cyclotron-produced 99mTc using direct 100Mo(p,2n) reaction

Evaluation of the radioisotopic purity of technetium-99m (99mTc) produced in GBq amounts by proton bombardment of enriched molibdenum-100 (100Mo) metallic targets at low proton energies (i.e. within 15\u201320 MeV) is conducted. This energy range was chosen since it is easily achievable by many conventional medical cyclotrons already available in the nuclear medicine departments of hospitals. The main motivation for such a study is in the framework of the research activities at the international level that have been conducted over the last few years to develop alternative production routes for the most widespread radioisotope used in medical imaging. The analysis of technetium isotopes and isomeric states (9xTc) present in the pertechnetate saline Na99mTcO4 solutions, obtained after the extraction/purification procedure, reveals radionuclidic purity levels basically in compliance with the limits recently issued by European Pharmacopoeia 9.3 (2018 Sodium pertechnetate (99mTc) injection 4801\u20133). Moreover, the impact of 9xTc contaminant nuclides on the final image quality is thoroughly evaluated, analyzing the emitted high-energy gamma rays and their influence on the image quality. The spatial resolution of images from cyclotron-produced 99mTc acquired with a mini-gamma camera was determined and compared with that obtained using technetium-99m solutions eluted from standard 99Mo/99mTc generators. The effect of the increased image background contribution due to Compton-scattered higher-energy gamma rays (E \u3b3 \u2009\u2009>\u2009\u2009200\u2009keV), which could cause image-contrast deterioration, was also studied. It is concluded that, due to the high radionuclidic purity of cyclotron-produced 99mTc using 100Mo(p,2n)99mTc reaction at a proton beam energy in the range 15.7\u201319.4 MeV, the resulting image properties are well comparable with those from the generator-eluted 99mTc

Utilização de chuveiros na sala pré-ordenha e sua influência na produtividade de búfalas da raça Murrah (Bubalus bubalis).

O estudo foi realizado com 64 búfalas, em dois tratamentos (1 e 2), durante seis dias. No primeiro, antes da ordenha, os animais permaneceram 1h em sala de espera com acesso ao chuveiro No segundo, os animais não tiveram acesso ao chuveiro. Foram registrados Índice de Temperatura de Globo e Umidade (ITGU) e a Umidade Relativa (UR) das salas de ordenha e de espera, Temperatura de Corpo (TC), Frequência Respiratória (FR), Reatividade (REAT), Nível de Estresse (NEST) e a Produção Diária de Leite (PL). Os dados foram analisados com o auxílio do programa SAS. O efeito da FR não foi significativo (P>0,57) sobre a PL. Não houve diferença significativa na PL para a REAT. O NEST foi o mesmo nos dois tratamentos. As regressões da característica (PL_AJ) sobre ITGU Interno (ITGU_I), ITGU Externo (ITGU_E), TC e UR não foram significativas (P>0,12). No tratamento 2 foram observados maior número de animais em classes de estresse, verificou-se que o tratamento 1 possibilitou a mudança ( alguns animais) da zona de alerta ou estresse para a zona de conforto térmico em relação ao ITGU_I. No tratamento 2, todos os animais estavam em estresse térmico. Os animais submetidos ao estresse calórico em ambas as situações utilizaram com eficiência o seu sistema termorregulador, sem interferir na produção de leite

THE TRIASSIC INSECTS FROM MONTE SAN GIORGIO: SYSTEMATICS AND PALEOENVIRONMENTAL IMPLICATIONS

A collection of 16 specimens of insects (both adult and larval forms) collected between 1997 and 2003 in the Lower Kalkschieferzone (KSZ) at the Val Mara site D near Meride (Canton Ticino, Svizzera), Monte San Giorgio by the UNIMI team lead by Prof. A. Tintori and Dr. C. Lombardo and with the support by Dr. Markus Felber, then curator at the Museo Cantonale di S.N. in Lugano (MCSN) are here described and the paleoenvironmental implications of this entomofauna is discussed. Monte San Giorgio is a Triassic fossil lagerst\ue4tte inscribed since 2003 in the UNESCO World Heritage for its vertebrate fossils, but the importance of its fossil insects is coming into light. The insect assemblage has proved to be exceptional under two aspects: it includes the most ancient fossil record for four groups and it provides the most ancient record of nervous structures (cerebrum and nerve cord) in insects, thanks to preservation through phosphatization. The Kalkschieferzone entomofauna is also quite diverse, including representatives of seven orders, both terrestrial and aquatic, suggesting the existence of a much more complex and stable terrestrial habitat than a carbonatic platform could support

Basic Course Leadership: Operational Transparency as a Best Practice for Adjunct Faculty Management

Research suggests adjunct faculty receive little institutional support and feel a sense of disconnection from their campuses. Nearly half of all faculty at American colleges and universities are classified as adjunct faculty in recent National Center for Educational Statistics reporting. Thus, academic departments should consider strategies to better include and engage adjunct faculty on their campuses. This article explores transparency as a best practice for the administration of communication basic courses at a mid-sized, regional university. Further, adjunct faculty members’ perceptions of the basic course administrators’ transparency will be discussed

The effectiveness of protection systems toward rockfall risk mitigation

Hazard

Undetected patricide: Inaccuracy of cause of death determination without an autopsy

Patricide (killing the father) is uncommon form of homicide. Usually the assaults occur at home in the absence of witnesses and adult sons are frequently involved. Homicides in a domestic context usually do not tend to recurrence, because the motivation for the crime ends with the death of the parent. However, this is not what was observed in the present case study dealing with the death of a 70 years old white man originally misclassified as accident and discovered three years later only after an additional homicide in a family context of a 60 years old white lady. Multiple stab wounds to the neck and thorax were misinterpreted at the external male body examination as blunt trauma falling down stairs. No forensic autopsy was requested and no comparison of medical findings with the results from the death scene, such as a bloodstain analysis was performed by the police officers nor required by the judicial authority. This was quite surprising because an additional but preliminary post-mortem external examination performed by a general practitioner on the male body already raised the suspicion that the external lesions were stab wounds thus requiring a forensic autopsy. Only the exhumation of the elderly body, performed years later, confirmed the diagnostic hypothesis raised by the first physician. The present case is quite representative of a death investigation not run professionally and performed by individuals with no specific training where most of the medico-legal investigations (especially for traumatic and violent deaths) are restricted to an external body examination without subsequent autopsy. Although misinterpretation of external lesions is inevitable and significant discrepancies between external body examination and forensic autopsy are not rare, in the case of contradictory results of postmortem external examination or unclear/suspicious cause and manner of death, investigation should proceed necessarily with a forensic autopsy

Penetrance of the V203I variant of the PRNP gene: report of a patient with stroke-like onset of Creutzfeld-Jacob Disease and review of published cases

open6noCreutzfeldt-Jakob disease (CJD) is usually sporadic, but 10-15% of cases are caused by autosomal-dominant pathogenic variants in the prion protein gene (PRNP). A few PRNP variants show low penetrance. We report the case of a 64-year-old man, admitted to the ward with acute onset of aphasia; death occurred 6 weeks later. Brain MRI, EEG pattern and brain pathology were consistent with CJD diagnosis. Genetic analysis revealed a heterozygous V203I variant. We summarized the key clinical findings in patients carrying the V203I variant who were described to date. We also discuss the hypothesis as to whether V203I is a risk factor for CJD rather than a Mendelian disease-associated variant, as well as the possible implications of such hypothesis in the clinical scenario.openGandoglia I.; Strada L.; Poleggi A.; Castaldi A.; Del Sette M.; Di Maria E.Gandoglia, I.; Strada, L.; Poleggi, A.; Castaldi, A.; Del Sette, M.; Di Maria, E

The Middle Triassic lagerstätt e of Monte San Giorgio reveals the oldest lace bugs (Hemiptera: Tingidae): Archetingis ladinica gen. n. sp. n.

A new genus and species of fossil lace bugs (Hemiptera; Tingidae), Archetingis ladinica gen. n. et sp. n. is described from the lower Kalkschieferzone (Meride Limestone, Upper Ladinian) of the Swiss side of Monte San Giorgio. The new taxon clearly resembles modern Tingidae in its hemelytra and pronotum, with two rows of areole, in the presence of bucculae closed at the anterior end, not extending beyond the apex of the head, and in the presence of a labial groove on the meso-/metathoracic sternum. Distinctive features are the thick femurs of the first and second pair of legs, the exceptional size (total length of ~12 mm) if compared with both fossil and extant species. Archetingis ladinica gen. n. et sp. n. represents by far the oldest known species of this family and brings back the origin of Tingidae of approximately 140 My, well into the Middle Triassic. The discovery of A. ladinica, beside its evolutionary consequences on the origin of extant Tingidae, provides evidences for the presence of terrestrial ecosystems nearby to the depositional environment. According to the living and trophic behavior of extant Tingidae, those emerged lands had to be covered by vegetation