Min-oscillations in Escherichia coli induced by interactions of membrane-bound proteins

During division it is of primary importance for a cell to correctly determine the site of cleavage. The bacterium Escherichia coli divides in the center, producing two daughter cells of equal size. Selection of the center as the correct division site is in part achieved by the Min-proteins. They oscillate between the two cell poles and thereby prevent division at these locations. Here, a phenomenological description for these oscillations is presented, where lateral interactions between proteins on the cell membrane play a key role. Solutions to the dynamic equations are compared to experimental findings. In particular, the temporal period of the oscillations is measured as a function of the cell length and found to be compatible with the theoretical prediction.Comment: 17 pages, 5 figures. Submitted to Physical Biolog

Positional information readout in Ca2+Ca^{2+} signaling

Living cells respond to spatial signals. Signal transmission to the cell interior often involves the release of second messengers like $Ca^{2+}$ . They will eventually trigger a physiological response by activating kinases that in turn activate target proteins through phosphorylation. Here, we investigate theoretically how positional information can be accurately read out by protein phosphorylation in spite of rapid second messenger diffusion. We find that accuracy is increased by binding of the kinases to the cell membrane prior to phosphorylation and by increasing the rate of $Ca^{2+}$ loss from the cell interior. These findings could explain some salient features of conventional protein kinases C

On uniformly rotating fluid drops trapped between two parallel plates

This contribution is about the dynamics of a liquid bridge between two fixed parallel plates. We consider a mathematical model and present some results from the doctoral thesis [10] of the first author. He showed that there is a Poisson bracket and a corresponding Hamiltonian, so that the model equations are in Hamiltonian form. The result generalizes previous results of Lewis et al. on the dynamics of free boundary problems for "free" liquid drops to the case of a drop between two parallel plates, including, especially the effect of capillarity and the angle of contact between the plates and the free fluid surface. Also, we prove the existence of special solutions which represent uniformly rotating fluid ridges, and we present specific stability conditions for these solutions. These results extend work of Concus and Finn [2] and Vogel [18],[19] on static capillarity problems (see also Finn [5]). Using the Hamiltonian structure of the model equations and symmetries of the solutions, the stability conditions can be derived in a systematic way. The ideas that are described will be useful for other situations involving capillarity and free boundary problems as well

Information Content in Data Sets for a Nucleated-Polymerization Model

We illustrate the use of tools (asymptotic theories of standard error quantification using appropriate statistical models, bootstrapping, model comparison techniques) in addition to sensitivity that may be employed to determine the information content in data sets. We do this in the context of recent models [23] for nucleated polymerization in proteins, about which very little is known regarding the underlying mechanisms; thus the methodology we develop here may be of great help to experimentalists

Age and growth of spiny dogfish (Squalus acanthias) in the Gulf of Alaska: analysis of alternative growth models

Ten growth models were fitted to age and growth data for spiny dogfish (Squalus acanthias) in the Gulf of Alaska. Previous studies of spiny dogfish growth have all fitted the t0 formulation of the von Bertalanffy model without examination of alternative models. Among the alternatives, we present a new two-phase von Bertalanffy growth model formulation with a logistically scaled k parameter and which estimates L0. A total of 1602 dogfish were aged from opportunistic collections with longline, rod and reel, set net, and trawling gear in the eastern and central Gulf of Alaska between 2004 and 2007. Ages were estimated from the median band count of three independent readings of the second dorsal spine plus the estimated number of worn bands for worn spines. Owing to a lack of small dogfish in the samples, lengths at age of small individuals were back-calculated from a subsample of 153 dogfish with unworn spines. The von Bertalanffy, two-parameter von Bertalanffy, two-phase von Bertalanffy, Gompertz, two-parameter Gompertz, and logistic models were fitted to length-at-age data for each sex separately, both with and without back-calculated lengths at age. The two-phase von Bertalanffy growth model produced the statistically best fit for both sexes of Gulf of Alaska spiny dogfish, resulting in L∞ = 87.2 and 102.5 cm and k= 0.106 and 0.058 for males and females, respectively

THE UNITED STATES IN THE GLOBAL SOYBEAN MARKET: WHERE DO WE GO FROM HERE?

This study applies the concept of a dynamic dominant-firm oligopoly model to the international soybean market. It has been suggested that the international soybean market should be viewed as an oligopoly among exporting nations. Consistent with Gaskins (1971) dynamic dominant firm model, our results indicate that the current U.S. loan deficiency-payment prices and their predecessors created an environment in which smaller (fringe) exporters could prosper and expand. The reduction of U.S. market share is thus a logical outcome of an "optimally managed decline" a la Gaskins. The study finds U.S. market share to decline at a reducing rate and predicts U.S. market share eventually to stabilize, given the expanding international market for soybeans and products. Recognition of the structure of international soybean market has policy implications for the 2002 farm program as the classic dominant firm model suggests.Crop Production/Industries, International Relations/Trade,

Some Eocene Dicotyledonous Woods from Eden Valley, Wyoming

Author Institution: Department of Botany, University of Cincinnati, Cincinnati 21, Ohi

Some Reactions of 4-Chlorocinnoline

This problem involves the preparation of 4-chlorocinnoline by the method of Borshe and Herbert and Busch and Rast,, and the study of further replacement reactions of the chlorine atom in the 4-position. The problem can be subdivided into two sections: (1) the preparation of the 4-cinnolyl Grignard reagent and the corresponding lithium compound and (2) the study of some condensation reactions of 4-chlorocinnoline