Subjective localization of electrocutaneous stimuli

Studying the perception of spatiotemporal stimulus patterns in various modalities may yield important information on the way in which humans process sensory information. The perception of tactile and nociceptive cutaneous stimulus patterns have been studied by Stolle et al. [1] and Trojan et al. [2][4] respectively. Among other things, both authors studied subjective localization of single stimuli. In Trojan et al. [4], two types of mislocalization patterns were observed for nociceptive single stimuli when comparing the localization reports with the stimulus locations: (1) overall proximal or distal displacement and (2) expansion or contraction of the stimulus area.\ud It is unknown whether tactile and nociceptive stimuli at the same skin site are perceived as being at the same site. Therefore, comparing the spatial perception of tactile and nociceptive cutaneous stimuli may provide new insights into their processing. This comparison can only be successfully made by applying nociceptive and tactile stimuli at the same skin site in the same experiment. This can be done by using a device which has recently been developed at our institute and which we refer to as the bimodal stimulation electrode [3]. \ud Recording the perceived locations of stimuli can be done by letting subjects report these on a scale. The most intuitive scale for this is the stimulated arm itself. However, this would bias the perception of stimulus location by providing visual information of the electrode locations. The goal of the present research was to (1) create and (2) test a setup which allows subjects to report perceived stimulus locations on their own arm without seeing the electrode positions. This was achieved by building a setup consisting of a touch screen (Provision Visboard) which presents a digital image of the subject’s own arm (without electrodes) and which is positioned over this arm after the electrodes have been attached. Subjects can report the localizations by pointing at the screen using a pointer

Influence of functional rider and horse asymmetries on saddle force distribution during stance and in sitting trot

Asymmetric forces exerted on the horse's back during riding are assumed to have a negative effect on rider–horse interaction, athletic performance, and health of the horse. Visualized on a saddle pressure mat, they are initially blamed on a nonfitting saddle. The contribution of horse and rider to an asymmetric loading pattern, however, is not well understood. The aim of this study was to investigate the effects of horse and rider asymmetries during stance and in sitting trot on the force distribution on the horse's back using a saddle pressure mat and motion capture analysis simultaneously. Data of 80 horse-rider pairs (HRP) were collected and analyzed using linear (mixed) models to determine the influence of rider and horse variables on asymmetric force distribution. Results showed high variation between HRP. Both rider and horse variables revealed significant relationships to asymmetric saddle force distribution (P < .001). During sitting trot, the collapse of the rider in one hip increased the force on the contralateral side, and the tilt of the rider's upper body to one side led to more force on the same side of the pressure mat. Analyzing different subsets of data revealed that rider posture as well as horse movements and conformation can cause an asymmetric force distribution. Because neither horse nor rider movement can be assessed independently during riding, the interpretation of an asymmetric force distribution on the saddle pressure mat remains challenging, and all contributing factors (horse, rider, saddle) need to be considered

Assessment and visualisation of daily-life arm movements after stroke

For an optimal guidance of the rehabilitation therapy of stroke patients in an in-home setting, objective, and patient specific assessment of upper extremity task performance is needed. Towards this goal, metrics of hand position relative to the pelvis were estimated and visualized. Metrics, including work area and maximum reaching distance, appeared to strongly correlate with the upper extremity part of the Fugl-Meyer Assessment scale (r>0.84, p<0.001). Proposed metrics and visualisation can be used to objectively assess the arm movement performance over a longer period of time in a daily-life setting, if combined with info about performed task derived from a activity monitor

Challenges and opportunities in mobile e-coaching

Background: Mobile e-health technologies have proven to provide tailored assessment, intervention, and coaching capabilities for various usage scenarios. Thanks to their spread and adoption, smartphones are one of the most important carriers for such applications.Problem: However, the process of design, realization, evaluation, and implementation of these e-health solutions is wicked and challenging, requiring multiple stakeholders and expertise.Method: Here, we present a tailorable intervention and interaction e-health solution that allows rapid prototyping, development, and evaluation of e-health interventions at scale. This platform allows researchers and clinicians to develop ecological momentary assessment, just-in-time adaptive interventions, ecological momentary intervention, cohort studies, and e-coaching and personalized interventions quickly, with no-code, and in a scalable way.Result: The Twente Intervention and Interaction Instrument (TIIM) has been used by over 320 researchers in the last decade. We present the ecosystem and synthesize the main scientific output from clinical and research studies in different fields.Discussion: The importance of mobile e-coaching for prediction, management, and prevention of adverse health outcomes is increasing. A profound e-health development strategyand strategic, technical, and operational investments are needed to prototype, develop, implement, and evaluate e-health solutions. TIIM ecosystem has proven to support these processes. This paper ends with the main research opportunities in mobile coaching, including intervention mechanisms, fine-grained monitoring, and inclusion of objective biomarker data

Weak negative associations between avian influenza virus infection and movement behaviour in a key host species, the mallard Anas platyrhynchos

Animal movements may contribute to the spread of pathogens. In the case of avian influenza virus, [migratory] birds have been suggested to play a role in the spread of some highly pathogenic strains (e.g. H5N1, H5N8), as well as their low pathogenic precursors which circulate naturally in wild birds. For a better understanding of the emergence and spread of both highly pathogenic (HPAIV) and low pathogenic avian influenza virus (LPAIV), the potential effects of LPAIVs on bird movement need to be evaluated. In a key host species, the mallard Anas platyrhynchos, we tested whether LPAIV infection status affected daily local ( 100 m) movements by comparing movement behaviour 1) within individuals (captured and sampled at two time points) and 2) between individuals (captured and sampled at one time point). We fitted free-living adult males with GPS loggers throughout the autumn LPAIV infection peak, and sampled them for LPAIV infection at logger deployment and at logger removal on recapture. Within individuals, we found no association between LPAIV infection and daily local and regional movements. Among individuals, daily regional movements of LPAIV infected mallards in the last days of tracking were lower than those of non-infected birds. Moreover, these regional movements of LPAIV infected birds were additionally reduced by poor weather conditions (i.e. increased wind and/or precipitation and lower temperatures). Local movements of LPAIV infected birds in t

A System for Monitoring Stroke Patients in a Home Environment

Currently, the changes of functional capacity and performance of stroke patients after returning home from a rehabilitation hospital is unknown for a physician, having no objective information about the intensity and quality of a patient's daily-life activities. Therefore, there is a need to develop and validate an unobtrusive and modular system for objectively monitoring the stroke patient's upper and lower extremity motor function in daily-life activities and in home training. This is the main goal of the European FP7 project named “INTERACTION‿. A complete sensing system is developed, whereby Inertial Measurement Units (IMU), Knitted Piezoresistive Fabric (KPF) goniometers, KPF strain sensors, EMG electrodes and force sensors are integrated into a modular sensor suit designed for stroke patients. In this paper, we describe the systems architecture. Data from the sensors are captured wirelessly and stored in a remote secure database for later access and processing via portal technology. In collaboration with clinicians and engineers, clinical outcome measures were defined and the question of how to present the data on the web portal was addressed. The first implementation of the complete system includes a basic version of all components and is currently being extended to include all sensors within the INTERACTION system

Aspergillus strain typing in the genomics era

Multiple reasons may justify a need for strain typing purposes, but the most common reason is to delineate the epidemiological relationships between isolates. The availability of whole genome sequences has greatly influenced our ability to develop highly targeted and efficient strain typing methods fur these purposes. Some strain typing methods may serve dual goals: not only can they be used to discriminate between multiple isolates of a certain species, they can also aid in the recognition, identification, description and validation process of a fungal species

The role of tunneling in enzyme catalysis of C–H activation

AbstractRecent data from studies of enzyme catalyzed hydrogen transfer reactions implicate a new theoretical context in which to understand C–H activation. This is much closer to the Marcus theory of electron transfer, in that environmental factors influence the probability of effective wave function overlap from donor to acceptor atoms. The larger size of hydrogen and the availability of three isotopes (H, D and T) introduce a dimension to the kinetic analysis that is not available for electron transfer. This concerns the role of gating between donor and acceptor atoms, in particular whether the system in question is able to tune distance between reactants to achieve maximal tunneling efficiency. Analysis of enzyme systems is providing increasing evidence of a role for active site residues in optimizing the inter-nuclear distance for nuclear tunneling. The ease with which this optimization can be perturbed, through site-specific mutagenesis or an alteration in reaction conditions, is also readily apparent from an analysis of the changes in the temperature dependence of hydrogen isotope effects