Lignocellulosic Materials as Dye Adsorbents: Adsorption of Methylene Blue and Congo Red on Brewers’ Spent Grain

Brewers’ spent grain (BSG), a lignocellulosic waste material, was evaluated as a low-cost adsorbent for the removal of synthetic dyes methylene blue (MB) and Congo red (CR) from aqueous solutions in a batch process. Characterisation of the BSG was performed by chemical analysis, FTIR and SEM. The effects of contact time, initial dye concentration, adsorbent particle size, adsorbent concentration and pH on the adsorption process were investigated. High removal of both dyes ranging from 70 to over 90 % was achieved. It was shown that in both cases (MB and CR adsorption) the process could be interpreted in terms of Langmuir and Freundlich adsorption isotherms. The kinetics of the adsorption process was well described by the pseudo-second-order model. The results indicated the potential use of BSG as a low-cost adsorbent for MB and CR dye removal from aqueous solutions. This work is licensed under a Creative Commons Attribution 4.0 International License

Synthetic dyes decolourisation on agar plates by selected fungi

U ovom radu istražena je sposobnost obezbojenja sintetskih bojila (malahitno zelenilo MZ, kristal violet KV, fuksin F i metilensko modrilo MM) pomoću četiri vrste gljiva bijelog truljenja: P. chrysosporium CCBAS 570, T. versicolor CCBAS AG613, T. versicolor TV6 i C. subvermispora. Gljive su uzgajane pri 27 ⁰C 9 (10) dana na agarnim pločama s dodatkom bojila u koncentracijama 50, 100 i 150 mg L$^{-1}$. Zona rasta kolonija te zona obezbojenja (promjene boje) mjerene su (u dva međusobno okomita smjera) svaka 3 dana. MZ je snažno inhibiralo rast svih vrsta, ali su sve pokazale dobru sposobnost obezbojenja ovog bojila, što je vidljivo iz velikog indeksa obezbojenja (promjer obezbojenja/promjer kolonije) u rasponu od 2,42 do 6,04. Sposobnost djelomičnog obezbojenja KV pokazale su sve vrste gljiva, pri čemu je rast vrsta P. chrysosporium i C. subvermispora bio snažno inhibiran ovim bojilom. F je inhibirao rast odabranih gljiva samo tijekom prvih dana uzgoja. Sposobnost obezbojenja F pokazale su sve vrste, ali indeks obezbojenja nije bio velik (0,24 – 1). Inhibicija rasta ovim bojilom primijećena je samo u prvim danima uzgoja. MM nije inhibiralo rast gljiva, ali je djelomičnu sposobnost obezbojenja ovog bojila pokazala jedino vrsta T. versicolor TV6. Utvrđeno je kako primijenjene koncentracije bojila u podlozi statistički značajno utječu na rast kolonija gljiva, ali nije utvrđena statistički značajna razlika u sposobnosti obezbojenja.Four white rot fungi P. chrysosporium CCBAS 570, T. versicolor CCBAS AG613, T. versicolor TV6 and C. subvermispora were tested for their ability to decolourise synthetic dyes (Malachite Green MG, Crystal Violet CV, Fuchsine F and Methylene Blue MB). Fungal species were cultivated at 27 °C for 9 (10) days on Potato dextrose agar plates containing dyes at final concentrations of 50, 100 and 150 mg L$^{-1}$. The colony radial growth and the decolourisation zone (in two perpendicular directions) were measured every three days. Although all fungal strains were strongly inhibited by MG, high decolourisation index (decolourisation diameter/mycelial diameter) ranging from 2.42 to 6.04 for all tested species indicated very good decolourisation ability. CV strongly inhibited the growth of all fungi except P. chrysosporium. However, all species partially decolourised CV. F was also decolourised by all strains but the decrease in decolourisation efficiency was observed (decolourisation index ranging from 0.24 to 1). Inhibitory effect of F to fungal growth was observed only during the first cultivation days. Only T. versicolor TV6 partially decolourised MB, even though this dye did not inhibit the mycelial growth of the tested fungal strains. The influence of agar plate dye concentration on fungal growth was statistically significant, while no statistical significance was observed regarding the decolourisation ability

Removal of synthetic dye malachite green using Trametes versicolor mycelial pellets

U ovom radu istražena je mogućnost upotrebe micelijskih peleta gljive bijelog truljenja Trametes versicolor CCBAS AG 613 s ciljem uklanjanja sintetskog bojila malahitnog zelenila iz vodene otopine. Istraživan je utjecaj početne koncentracije biomase, koncentracije malahitnog zelenila i dodatka glukoze u otopinu bojila na postotak uklanjanja bojila. U gotovo svim provedenim pokusima postotak uklanjanja bojila nakon 24 h iznosio je preko 80 %. Povećanjem početne koncentracije biomase došlo je do povećanja postotka uklanjanja bojila; dok je povećanje koncentracije bojila, neovisno o početnoj koncentraciji biomase, imalo suprotan učinak. Dodatak glukoze u vodenu otopinu bojila rezultirao je manjim postotkom uklanjanja bojila. Nadalje, istražena je mogućnost ponovnog korištenja peleta u drugom ciklusu. U ponovljenom testu peleti su zadržali sposobnost uklanjanja bojila iz vodene otopine malahitnog zelenila, ali u nešto manjem postotku nego prilikom prvog korištenja. Ovi preliminarni rezultati upućuju na mogućnost korištenja micelijskih peleta T. versicolor CCBAS AG613 za biološku obradu otpadnih voda obojenih malahitnim zelenilom.The aim of this study was to investigate the malachite green removal (decolourisation) ability of white rot fungus Trametes versicolor CCBAS AG613 mycelial pellets. The effect of initial biomass concentration, malachite green concentration and glucose addition on dye percentage removal was investigated. The dye percentage removal of more than 80% was achieved after 24 h in almost all runs. An increase in the initial biomass concentration positively affected the removal, resulting in higher dye percentage removal. However, the increase in dye concentration had the opposite effect. The addition of glucose to the dye solution resulted in slightly lower dye percentage removal. Furthermore, the longevity of pellets’ decolourisation activity was tested in repeated-batch mode. When used in repeated-batch mode, pellets still exhibited the malachite green decolourisation activity but to a slightly lower extent. These preliminary results indicate that T. versicolor CCBAS AG613 mycelial pellets could be effectively used for bioremediation of malachite green coloured wastewaters

Ružička days : International conference 16th Ružička Days “Today Science – Tomorrow Industry” : Proceedings

Proceedings contains articles presented at Conference divided into sections: open lecture (1), chemical analysis and synthesis (3), chemical and biochemical engineering (8), food technology and biotechnology (8), medical chemistry and pharmacy (3), environmental protection (11) and meeting of young chemists (2)

Water for all : Proceedings of the 7th international scientific and professional conference Water for all

The 7th International Scientific and Professional Conference Water for all is organized to honour the World Water Day by the Josip Juraj Strossmayer University of Osijek, European Hygienic Engineering & Design Group (EHEDG), Danube Parks, Croatian Food Agency, Croatian Water, Faculty of Food Technology Osijek, Faculty of Agriculture in Osijek, Faculty of Civil Engineering Osijek, Josip Juraj Strossmayer University of Osijek Department of Biology, Josip Juraj Strossmayer University of Osijek Department of Chemistry, Nature Park “Kopački rit”, Osijek- Baranja County, Public Health Institute of the Osijek- Baranja County and „Vodovod-Osijek“ -water supply company in Osijek. The topic of World Water Day 2017 was "Wastewater" emphasizing the importance and influence of wastewater treatments on global environment. The international scientific and professional conference Water for all is a gathering of scientists and experts in the field of water management, including chemists, biologists, civil and agriculture engineers, with a goal to remind people about the significance of fresh water and to promote an interdisciplinary approach and sustainability for fresh water resource management. The Conference has been held since 2011. About 300 scientists and engineers submitted 95 abstracts to the 7th International Scientific and Professional Conference Water for all, out of which 33 was presented orally and 62 as posters. 47 full papers were accepted by the Scientific Committee. 38 full papers became the part of the this Proceedings while 9 papers were accepted for publication in Croatian Journal of Food Science and Technology and Electronic Journal of the Faculty of Civil Engineering Osijek - e-GFOS