Oligotyping input sequences part 3 of 3

Oligotyping input sequences part 3 of

QIIME input sequences part 1 of 3

QIIME input sequences part 1 of

QIIME input sequences part 3 of 3

QIIME input sequences part 3 of

Raw data files and R script

Raw data files and R scrip

Oligotyping input sequences part 1 of 3

Oligotyping input sequences part 1 of

QIIME input file

Filtered and joined FASTA sequences for QIIME input

de-multiplexing scripts

Each demultiplexing script corresponds to a particular input dataset. demultiplex1.pl: Water, larvae, PBM and NBF Ae. agypti; demultiplex2.pl: Water (An. gambiae and Gr. atropalpus), sterilized and non-sterilized eggs (Ae. aegypti). demultiplex3.pl: Larvae (An. gambiae and Gr. atropalpus)

data_files_and_r_script

Data collected for larval survival, development and feeding assays, as well as R code used to perform statistical analyses

QIIME input file

Filtered and joined FASTA sequences for QIIME input

Transcriptome Sequencing Reveals Large-Scale Changes in Axenic <i>Aedes aegypti</i> Larvae

<div><p>Mosquitoes host communities of microbes in their digestive tract that consist primarily of bacteria. We previously reported that <i>Aedes aegypti</i> larvae colonized by a native community of bacteria and gnotobiotic larvae colonized by only <i>Escherichia coli</i> develop very similarly into adults, whereas axenic larvae never molt and die as first instars. In this study, we extended these findings by first comparing the growth and abundance of bacteria in conventional, gnotobiotic, and axenic larvae during the first instar. Results showed that conventional and gnotobiotic larvae exhibited no differences in growth, timing of molting, or number of bacteria in their digestive tract. Axenic larvae in contrast grew minimally and never achieved the critical size associated with molting by conventional and gnotobiotic larvae. In the second part of the study we compared patterns of gene expression in conventional, gnotobiotic and axenic larvae by conducting an RNAseq analysis of gut and nongut tissues (carcass) at 22 h post-hatching. Approximately 12% of <i>Ae</i>. <i>aegypti</i> transcripts were differentially expressed in axenic versus conventional or gnotobiotic larvae. However, this profile consisted primarily of transcripts in seven categories that included the down-regulation of select peptidases in the gut and up-regulation of several genes in the gut and carcass with roles in amino acid transport, hormonal signaling, and metabolism. Overall, our results indicate that axenic larvae exhibit alterations in gene expression consistent with defects in acquisition and assimilation of nutrients required for growth.</p></div