First record of a spindle cowry Crenovolva (Ovulidae, Gastropoda) from Bulleji, Karachi, on the gorgonian coral Muricella sp. and with a note on their association

Attached to hard substrata on our shores occur the branching colonies of gorgonian corals of the genus Muricella (Fig. 1A, Pl. 1B, C, D). Since there is no report on this genus from Pakistan this report be taken as the first record from here. The family Ovulidae comprises several hundred species of marine gastropods, all of which appear to be obligate associates of cnidarians as stated by Robertson (1970) and Hadfield (1976); the present ovulid gastropod genus Crenovolva lives exclusively on the gorgonians (Morton, 1989). Tirmizi and Zehra (1984) recorded thirty-two families of gastropods from Pakistan, but not any representative of the Ovulidae, so the material at hand may be the first record from Pakistan. Since we are unable to reach to specific level the material is being reported as unspecified

On the rediscovery of Baseodiscus hemprichii (Ehrenberg, 1831)(Nemertea, Anopla, Baseodiscidae) from Karachi waters

During a study of biodiversity in the Pakistan coast several species of nemerteans were encountered. One is uncertainly identified as Lineus ruber (Muller, 1774), the identification of another is under way. A third, Baseodiscus hemprichii, has previously been recorded from Karachi by Puri (1924). He gave a detailed description of his specimens, but no illustrations. Baseodiscus hemprichii has been collected many times from rocky areas on the Karachi coast, but on only one occasion was it photographed and then drawn. The species is briefly described here

Non-Functional Requirements Elicitation Guideline for Agile Methods

One of the essential activities in software development is elicitation of requirement. Majority of the studies has pointed out that less attention is given to the NonFunctional Requirement (NFR). The negligence of NFR elicitation is due to lack of knowledge of the user and developer about NFR. Our study presents elicitation guidelines for NFRs in agile methods. This guideline will helps developers as well as users in agile methods. A case study is conducted on the group of master students for eliciting NFR with the help of elicitation guidelines. In addition, the initial results were obtained by extracting NFRs from eProcurement document that carries requirements of major European Union projects. The result of the case study is positive and encouraging for the new developers and users having less awareness about NFRs. Furthermore, the study describes the role of cloud computing in agile methods, especially in elicitation activity

Association of Physical Activity with Co-morbid Conditions in Geriatric Population

To find out association of physical activity with co-morbid conditions in geriatric population, a cross-sectional study was conducted in different cties of Pakistan in 2015. A total of 114 participants were inducted by non-probability convenience sampling technique. Data was collected after informed verbal consent by a validated questionnaire that is Rapid Assessment of Physical Activity (RAPA). Participants were categorized into two groups i.e. physically active and physically inactive. Data was entered and analyzed in SPSS version 20. There were 66 (57.9%) males and 48 (42.1%) females with mean age of 57.04±7.348 years. Among hypertensive individuals (n=43, 37.7%) there were 39 (90.7%) physically inactive, among individuals having angina (n=17, 14.9%) there were 15 (88.2%) physically inactive. Out of 37 (32.5%) diabetics, 35 (94.6%) were physically inactive. Among individuals suffering from arthritis (n=40, 35.1%), there were 38 (95%) physically inactive. A significant association was found between physical activity and diabetes and arthritis with p-value of 0.048 and 0.029 respectively. Physical activity is significantly associated with diabetes and arthritis in geriatric population. Adequate physical activity should be performed to reduce the risk of co-morbid conditions and improve the quality of life in geriatric population

N-acetyl-beta-D-glucosaminidase in acute myocardial infarction

The objective of the study was to investigate whether the lysosomal enzyme, N-Acetyl-beta-D-glucosaminidase (NAG) activity is increased in plasma of patients with acute myocardial infarction (AMI) and to determine if there is any association between plasma levels of NAG and severity of myocardial infarction (MI). NAG activity in plasma was monitored in 69 patients with AMI and 135 normal healthy subjects using a spectrofluorimetric method. A modified Aldrich ST elevation score was used to gauge the severity of MI in terms of size of the infarct. Plasma NAG levels in AMI patients and normal healthy subjects were found to be 10.92+/-7.5 U/l and 6.8+/-2.2 U/l, respectively. These two mean value when compared by Student\u27s t-test were significantly different P = 0.0001. No statistically significant differences in NAG activity were observed in patients in terms of gender, age, location of infarct, time from onset of chest pain to blood sampling in the hospital and size of the infarct

Drug resistance profile and biofilm forming potential of Pseudomonas aeruginosa isolated from contact lenses in Karachi-Pakistan

BACKGROUND: The contaminated contact lens provides Pseudomonas aeruginosa an ideal site for attachment and biofilm production. Continuous contact of the eye to the biofilm-infested lens can lead to serious ocular diseases, such as keratitis (corneal ulcers). The biofilms also prevent effective penetration of the antibiotics, which increase the chances of antibiotic resistance. METHODS: For this study, 22 Pseudomonas aeruginosa isolates were obtained from 36 contact lenses and 14 contact lens protective fluid samples. These isolates were tested against eight commonly used antibiotics using Kirby-Bauer disk diffusion method. The biofilm forming potential of these isolates was also evaluated using various qualitative and quantitative techniques. Finally, a relationship between biofilm formation and antibiotic resistance was also examined. RESULTS: The isolates of Pseudomonas aeruginosa tested were found resistant to most of the antibiotics tested. Qualitative and quantitative biofilm analysis revealed that most of the isolates exhibited strong biofilm production. The biofilm production was significantly higher in isolates that were multi-drug resistant (p < 0.0001). CONCLUSION: Our study indicates that multi-drug resistant, biofilm forming Pseudomonas aeruginosa isolates are mainly involved in contact lens associated infections. This appears to be the first report from Pakistan, which analyzes both antibiotic resistance profile and biofilm forming potential of Pseudomonas aeruginosa isolates from contact lens of the patients with contact lens associated infections

Plants and Small Molecules: An Up-and-Coming Synergy

The emergence of Arabidopsis thaliana as a model system has led to a rapid and wide improvement in molecular genetics techniques for studying gene function and regulation. However, there are still several drawbacks that cannot be easily solved with molecular genetic approaches, such as the study of unfriendly species, which are of increasing agronomic interest but are not easily transformed, thus are not prone to many molecular techniques. Chemical genetics represents a methodology able to fill this gap. Chemical genetics lies between chemistry and biology and relies on small molecules to phenocopy genetic mutations addressing specific targets. Advances in recent decades have greatly improved both target specificity and activity, expanding the application of this approach to any biological process. As for classical genetics, chemical genetics also proceeds with a forward or reverse approach depending on the nature of the study. In this review, we addressed this topic in the study of plant photomorphogenesis, stress responses and epigenetic processes. We have dealt with some cases of repurposing compounds whose activity has been previously proven in human cells and, conversely, studies where plants have been a tool for the characterization of small molecules. In addition, we delved into the chemical synthesis and improvement of some of the compounds described

Integration of Distributed Generations in Smart Distribution Networks Using Multi-Criteria Based Sustainable Planning Approach

Energy planning has become more complicated in the 21st century of sustainable development due to the inclusion of numerous standards such as techno-economic, and environmental considerations. This paper proposes multi-criteria sustainable planning (MCSP) based optimization approach for identifying DGs’ optimal allocations and rating powers. The main objectives of this paper are the reduction of the network’s total power loss, voltage profile improvement, energy loss saving maximization, and curtailing environmental emissions and water consumption to achieve Sustainable Development Goals (SDGs 3, 6, 7, 13, and 15) by taking the constraints into consideration. Different alternatives are evaluated across four aspects of performance indices; technical, cost-economic, environmental, and social (TEES). In terms of TEES performance evaluations, various multi-criteria decision-making (MCDM) approaches are used to determine the optimal trade-off among the available solutions. These methods are gaining wide acceptance due to their flexibility while considering all criteria and objectives concurrently. Annual energy loss saving is increased by 97.13%, voltage profile is improved to 0.9943 (p.u), and emissions are reduced by 82.45% using the proposed technique. The numerical results of the proposed MCSP approach are compared to previously published works to validate and may be used by researchers and energy planners as a planning tool for ADN schemes