CRISPR-KRISPR: A Method to Identify On-Target and Random Insertion of Donor DNAs and their Characterization in Knock-In Mice

CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target lesions and random insertions, using tail or earpiece DNA, are unavailable. We develop CRISPR-KRISPR (CRISPR-Knock-ins and Random Inserts Searching PRotocol), a method to identify both off-target lesions and random insertions. CRISPR-KRISPR uses as little as 3.4 μg of genomic DNA; thus, it can be easily incorporated as an additional step to genotype founder animals for further breeding

JASMINE: Near-infrared astrometry and time-series photometry science

The Japan Astrometry Satellite Mission for INfrared Exploration (JASMINE) is a planned M-class science space mission by the Institute of Space and Astronautical Science, the Japan Aerospace Exploration Agency. JASMINE has two main science goals. One is Galactic archaeology with a Galactic Center survey, which aims to reveal the Milky Way’s central core structure and formation history from Gaia-level (∼25 ${\mu}$as) astrometry in the near-infrared (NIR) Hw band (1.0–1.6 ${\mu}$m). The other is an exoplanet survey, which aims to discover transiting Earth-like exoplanets in the habitable zone from NIR time-series photometry of M dwarfs when the Galactic Center is not accessible. We introduce the mission, review many science objectives, and present the instrument concept. JASMINE will be the first dedicated NIR astrometry space mission and provide precise astrometric information on the stars in the Galactic Center, taking advantage of the significantly lower extinction in the NIR. The precise astrometry is obtained by taking many short-exposure images. Hence, the JASMINE Galactic Center survey data will be valuable for studies of exoplanet transits, asteroseismology, variable stars, and microlensing studies, including discovery of (intermediate-mass) black holes. We highlight a swath of such potential science, and also describe synergies with other missions

Differences in the Approach to School Disaster Education between Japan and the UK

In order to build a resilient society, it is necessary to implement the integrated disaster management in each community. Appropriate knowledge and skills are necessary to implement disaster management individually. Hence chances of disaster education should be given for community members. Each community has schools and most children can go to schools. Thus, improving school disaster education is effective to spread the appropriate knowledge of disaster management. Some of the countries which have many natural hazards such as Japan and US usually do separated disaster education. For instance disaster education includes only education for natural hazards in Japan. Traffic safety education and anticrime education are not included in disaster education. On the other hand, British disaster education is different from this approach. Their approach to disaster education is holistic approach. It includes all type of hazards, thus it should be called not disaster education but risk education or safety education. In this paper, a comparison between Japan and UK on school disaster education is introduced. It is based on results from the series of seminars in the UK in 2007. This paper focuses on the difference in the approach to school disaster education