Coordinates of features on the Galilean satellites

The coordinate systems of each of the Galilean satellites are defined and coordinates of features seen in the Voyager pictures of these satellites are presented. The control nets of the satellites were computed by means of single block analytical triangulations. The normal equations were solved by the conjugate iterative method which is convenient and which converges rapidly as the initial estimates of the parameters are very good

Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal.

Protein trafficking between the endoplasmic reticulum (ER) and Golgi apparatus is central to cellular homeostasis. ER export signals are utilized by a subset of proteins to rapidly exit the ER by direct uptake into COPII vesicles for transport to the Golgi. Norwalk virus nonstructural protein p22 contains a YXΦESDG motif that mimics a di-acidic ER export signal in both sequence and function. However, unlike normal ER export signals, the ER export signal mimic of p22 is necessary for apparent inhibition of normal COPII vesicle trafficking, which leads to Golgi disassembly and antagonism of Golgi-dependent cellular protein secretion. This is the first reported function for p22. Disassembly of the Golgi apparatus was also observed in cells replicating Norwalk virus, which may contribute to pathogenesis by interfering with cellular processes that are dependent on an intact secretory pathway. These results indicate that the ER export signal mimic is critical to the antagonistic function of p22, shown herein to be a novel antagonist of ER/Golgi trafficking. This unique and well-conserved human norovirus motif is therefore an appealing target for antiviral drug development

Metastable liquid-liquid coexistence and density anomalies in a core-softened fluid

Linearly-sloped or `ramp' potentials belong to a class of core-softened models which possess a liquid-liquid critical point (LLCP) in addition to the usual liquid-gas critical point. Furthermore they exhibit thermodynamic anomalies in the density and compressibility, the nature of which may be akin to those occurring in water. Previous simulation studies of ramp potentials have focused on just one functional form, for which the LLCP is thermodynamically stable. In this work we construct a series of ramp potentials, which interpolate between this previously studied form and a ramp-based approximation to the Lennard-Jones (LJ) potential. By means of Monte Carlo simulation, we locate the LLCP, the first order high density liquid (HDL)-low density liquid (LDL) coexistence line, and the line of density maxima for a selection of potentials in the series. We observe that as the LJ limit is approached, the LLCP becomes metastable with respect to freezing into a hexagonal close packed crystalline solid. The qualitative nature of the phase behaviour in this regime shows a remarkable resemblance to that seen in simulation studies of accurate water models. Specifically, the density of the liquid phase exceeds that of the solid; the gradient of the metastable LDL-HDL line is negative in the pressure (p)-temperature (T) plane; while the line of density maxima in the p-T plane has a shape similar to that seen in water and extends well into the {\em stable} liquid region of the phase diagram. As such, our results lend weight to the `second critical point' hypothesis as an explanation for the anomalous behaviour of water.Comment: 7 pages, 8 figure

Reference gene validation for gene expression studies using quantitative RT-PCR during berry development of ‘Aki Queen’ grapes

In order to understand the gene regulation during berry development and examine the effect of abscisic acid (ABA) on gene expression related to berry maturation, we evaluated the validity of four housekeeping genes, elongation factor 1-α (EF1-α), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ubiquitin-conjugating enzyme (UBC), and 60S ribosomal protein L40-1 (VvUbiquitin1), as references by using 'Aki Queen' grapes with and without ABA treatment. The main contribution of this study is that a gene expression analysis using GAPDH as a reference gene will help to elucidate the berry development process and the physiological effects of ABA on berry maturation of 'Aki Queen' grapes

Transverse instability due to the space charge during the electron-cooling bunching of ion beams

A beam-tracking simulation has been done for study of the electron- cooling bunching of ion beams around a few hundred MeV/u. In the simulation, the field due to the space charge of a beam in a round vacuum chamber is calculated from the charge distribution of macro- particles and the image particles due to the macro-particles. The simulation results show that the bunched beams meet a transverse instability more easily than coasting beams. (2 refs)