GRAPEVINE: Grids about anything by Poisson's equation in a visually interactive networking environment

A proven 3-D multiple-block elliptic grid generator, designed to run in 'batch mode' on a supercomputer, is improved by the creation of a modern graphical user interface (GUI) running on a workstation. The two parts are connected in real time by a network. The resultant system offers a significant speedup in the process of preparing and formatting input data and the ability to watch the grid solution converge by replotting the grid at each iteration step. The result is a reduction in user time and CPU time required to generate the grid and an enhanced understanding of the elliptic solution process. This software system, called GRAPEVINE, is described, and certain observations are made concerning the creation of such software

MGMT enrichment and second gene co-expression in hematopoietic progenitor cells using separate or dual-gene lentiviral vectors

AbstractThe DNA repair gene O6-methylguanine-DNA methyltransferase (MGMT) allows efficient in vivo enrichment of transduced hematopoietic stem cells (HSC). Thus, linking this selection strategy to therapeutic gene expression offers the potential to reconstitute diseased hematopoietic tissue with gene-corrected cells. However, different dual-gene expression vector strategies are limited by poor expression of one or both transgenes. To evaluate different co-expression strategies in the context of MGMT-mediated HSC enrichment, we compared selection and expression efficacies in cells cotransduced with separate single-gene MGMT and GFP lentivectors to those obtained with dual-gene vectors employing either encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) or foot and mouth disease virus (FMDV) 2A elements for co-expression strategies. Each strategy was evaluated in vitro and in vivo using equivalent multiplicities of infection (MOI) to transduce 5-fluorouracil (5-FU) or Lin−Sca-1+c-kit+ (LSK)-enriched murine bone marrow cells (BMCs). The highest dual-gene expression (MGMT+GFP+) percentages were obtained with the FMDV-2A dual-gene vector, but half of the resulting gene products existed as fusion proteins. Following selection, dual-gene expression percentages in single-gene vector cotransduced and dual-gene vector transduced populations were similar. Equivalent MGMT expression levels were obtained with each strategy, but GFP expression levels derived from the IRES dual-gene vector were significantly lower. In mice, vector-insertion averages were similar among cells enriched after dual-gene vectors and those cotransduced with single-gene vectors. These data demonstrate the limitations and advantages of each strategy in the context of MGMT-mediated selection, and may provide insights into vector design with respect to a particular therapeutic gene or hematologic defect

1984 Ruby Yearbook

A digitized copy of the 1984 Ruby, the Ursinus College yearbook.https://digitalcommons.ursinus.edu/ruby/1087/thumbnail.jp

The Grizzly, March 19, 1982

New Room Selection Procedure Instituted • Thefts and Break-ins Challenge Campus Security • Board Activates New Program • Going to the Dogs? • USGA Notes • News Briefs: Special Olympics Returns: Hugger Needed!; Red Cross Bloodmobile Urges Volunteer Donors; Advertising Association Sponsors Career Conference • Newly Formed Astronomy Club Approved by SAC • Bach Birthday Concert • UC Track and Field: A Team to be Reckoned With • Blades Shred F&M • Lacrosse Looking for Improvement • Potsdam Frustrates UC Again • Women\u27s Basketball Takes Second in Cinderella MACs • Aqua Women Achieve Personal Best at MACs and Nationalshttps://digitalcommons.ursinus.edu/grizzlynews/1075/thumbnail.jp

A standard variation file format for human genome sequences

Here we describe the Genome Variation Format (GVF) and the 10Gen dataset. GVF, an extension of Generic Feature Format version 3 (GFF3), is a simple tab-delimited format for DNA variant files, which uses Sequence Ontology to describe genome variation data. The 10Gen dataset, ten human genomes in GVF format, is freely available for community analysis from the Sequence Ontology website and from an Amazon elastic block storage (EBS) snapshot for use in Amazon's EC2 cloud computing environment

2013-2014 Chamber Music Palm Beach No. 2

https://spiral.lynn.edu/conservatory_otherseasonalconcerts/1013/thumbnail.jp

2013-2014 Chamber Music Palm Beach No. 1

https://spiral.lynn.edu/conservatory_otherseasonalconcerts/1014/thumbnail.jp

Terminate Lung Cancer (TLC) Study—A Mixed-Methods Population Approach to Increase Lung Cancer Screening Awareness and Low-Dose Computed Tomography in Eastern Kentucky

For low dose CT lung cancer screening to be effective in curbing disease mortality, efforts are needed to overcome barriers to awareness and facilitate uptake of the current evidence-based screening guidelines. A sequential mixed-methods approach was employed to design a screening campaign utilizing messages developed from community focus groups, followed by implementation of the outreach campaign intervention in two high-risk Kentucky regions. This study reports on rates of awareness and screening in intervention regions, as compared to a control region

The Grizzly, February 12, 1982

Administration Keeping an Eye on Pledging • Housing System Under One Roof • Sororities Dance for Charity • UC College Bowl Impressive at Moravian • Comment: A Letter to Dr. Kane • USGA Notes • Legend Behind Lorelei • FCA Marathon for Special Olympics • Winterfest Tickets Available for Students • Forum on Rising Medical Costs • President\u27s Corner: The Biologist and the Poet • Concert Review: JT, Somehow Perfect; Cars, Shakin\u27 It Up • Vanities Auditions • Soft Core Culture • Frats Converge for Bid Day • Sports Briefs: W\u27s Swimming Gets Revenge; Aquabears Down Swarthmore; Dreamers and Jamas Undefeated • Matmen Run With Streak to Six • Women Crush Cabrini, 78-62 • Men\u27s B-Ball Takes Twohttps://digitalcommons.ursinus.edu/grizzlynews/1072/thumbnail.jp

The Grizzly, April 2, 1982

PA Special Olympics This Weekend • Room Selection Continues • Student Publications Staffs Chosen • Parents Day Plans Offer Variety of Talents • USGA Notes • Letters to the Editor • Dean of College Accepting Nominations for Lindback Award • Class Elections Next Week • News Briefs: Hooters Concert Tickets Now Available; Friends of Library Book Sale; Senior Women Invited to Parents Day Reception; Photography in Space Topic of Final Forum; Scholarship Offered for Students of Hellenic Descent • Food Day: A Chance to Share the Wealth • New Library Survey Conducted • Alumna Presents DuPont Grant • Students Direct Comedy Series • Duet Takes First in Talent Show • F&M Tops Track Team • W\u27s Tennis Drops Warm-up • Division 1 West Chester a Little Too Strong • MAC Champs Back • Harvard Slips by Lady Bears • Batsmen Split Twin Bill • Softball Team Takes Two Out of Threehttps://digitalcommons.ursinus.edu/grizzlynews/1077/thumbnail.jp