624 research outputs found

    Pengaruh Leaders Personality terhadap Employee Satisfaction melalui Leader Member Exchange sebagai Variabel Intervening pada Departemen Support PT Pakuwon Jati Surabaya

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    Pengaruh leaders personality terhadap employee satisfaction melalui leader member exchange sebagai variabel intervening pada departemen support PT Pakuwon Jati Surabaya.Penelitian ini dilakukan bertujuan untuk mengetahui apakah leaders personality berpengaruh terhadap employee satisfaction melalui leader member exchange sebagai variabel intervening pada departemen support PT Pakuwon Jati Surabaya. Jenis penelitian ini menggunakan pendekatan kuantitatif. Sampel yang digunakan dalam penelitian ini adalah sebanyak 88 responden. Data didapat dengan menyebarkan angket.Hasil penelitian ini menunjukkan bahwa leaders personality berpengaruh secara signifikan terhadap employee satisfaction, leader member exchange berpengaruh secara signifikan terhadap employee satisfaction, dan leaders personality berpengaruh secara signifikan terhadap leader member exchange

    Upaya Konsultan Pajak Dalam Memenangkan Kasus Banding Dan Gugatan Dalam Perpajakan

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    Penelitian ini bertujuan untuk mengetahui upaya kuasa hukum wajib pajak dalam memenangkan kasus banding dan gugatan. Penelitian ini menggunakan metode kualitatif, karena harus melakukan wawancara yang mendalam terhadap responden. Penelitian ini dilakukan dengan melakukan wawancara terhadap konsultan dan kuasa hukum wajib pajak yang pernah mengalami menang dan kalah dalam persidangan, karena konsultan dan kuasa hukum wajib pajak yang pernah mengalami menang dan kalah dalam persidangan memiliki pengalaman apa saja yang dapat membuat menang dalam persidangan dan apa saja yang membuat kalah dalam persidangan, dan hasil wawancara tersebut dibandingkan, apa yang membuat menang dan kalah dalam persidangan.Hasil penelitian ini menunjukkan bahwa tidak hanya kompetensi perpajakan saja yang digunakan untuk memenangkan persidangan banding dan gugatan. Terdapat faktor lain yang menunjang seorang konsultan pajak dan kuasa hukum wajib pajak dalam memenangkan kasus banding dan gugatan yaitu, faktor komunikasi, pengalaman kerja, disiplin kerja, relasi antara sesama kuasa hukum dan majelis hakim. Berbagai hal tersebut terbukti sangat berpengaruh dalam memenangkan sebuah kasus.

    Analisis Faktor-faktor Pembentuk Komponen Brand Experience Di Platinum Grill Surabaya

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    Penelitian ini dilakukan untuk menganalisis faktor-faktor Product Experience, Look and Feel, dan Experiential Communications sebagai pembentuk Brand Experience di Platinum Grill Surabaya. Penelitian ini merupakan penelitian kualitatif dengan metode wawancara mendalam terhadap 10 informan. Teknik analisa yang digunakan yaitu analisa triangulasi. Hasil penelitian menunjukkan bahwa faktor-faktor pembentuk Brand Experience berpengaruh di Platinum Grill Surabaya dan dapat memberikan kepuasan bagi konsumen. Berdasarkan analisa triangulasi disimpulkan bahwa faktor Experiential Communications paling berpengaruh dalam memberikan kepuasan konsumen

    Evaluating the effects of nacre on human skin and scar cells in culture

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    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.Pearl nacre, a biomineralisation product of molluscs, has growing applications in cosmetics, as well as dental and bone restoration, yet a systematic evaluation of its biosafety is lacking. Here, we assessed the biocompatibility of nacre with two human primary dermal fibroblast cell cultures and an immortalised epidermal cell line and found no adverse effects

    The ‘integrative approach’ and labour regulation and Indonesia: prospects and challenges

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    In contrast to theories of regulation which bypass the state and cede regulatory authority to private regimes, the scholar Kevin Kolben makes a cogent argument for the state to be brought back to centre stage in labour regulation, but envisages that private actors can develop and strengthen its capacity. This article considers the utility of what he terms an integrative approach for Indonesia. In line with what the approach advocates, the article examines the relationships between private actors and the state and considers the extent to which the former can communicate, interact with and incentivise the latter in ways which strengthen its regulatory capacity. Several challenges are identified. Finally, the potential of the Better Work Programme in Indonesia to further the goals of the approach is assessed

    Estimation of interdomain flexibility of N-terminus of factor H using residual dipolar couplings

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    Characterization of segmental flexibility is needed to understand the biological mechanisms of the very large category of functionally diverse proteins, exemplified by the regulators of complement activation, that consist of numerous compact modules or domains linked by short, potentially flexible, sequences of amino acid residues. The use of NMR-derived residual dipolar couplings (RDCs), in magnetically aligned media, to evaluate interdomain motion is established but only for two-domain proteins. We focused on the three N-terminal domains (called CCPs or SCRs) of the important complement regulator, human factor H (i.e. FH1-3). These domains cooperate to facilitate cleavage of the key complement activation-specific protein fragment, C3b, forming iC3b that no longer participates in the complement cascade. We refined a three-dimensional solution structure of recombinant FH1-3 based on nuclear Overhauser effects and RDCs. We then employed a rudimentary series of RDC datasets, collected in media containing magnetically aligned bicelles (disk-like particles formed from phospholipids) under three different conditions, to estimate interdomain motions. This circumvents a requirement of previous approaches for technically difficult collection of five independent RDC datasets. More than 80% of conformers of this predominantly extended three-domain molecule exhibit flexions of < 40 °. Such segmental flexibility (together with the local dynamics of the hypervariable loop within domain 3), could facilitate recognition of C3b via initial anchoring and eventual reorganization of modules to the conformation captured in the previously solved crystal structure of a C3b:FH1-4 complex

    MRI for assessment of anal fistula

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    Magnetic resonance imaging (MRI) is the best imaging modality for preoperative assessment of patients with anal fistula. MRI helps to accurately demonstrate disease extension and predict prognosis. This in turn helps make therapy decisions and monitor therapy. The pertinent anatomy, fistula classification and MRI findings will be discussed

    The Impact of Hydrogen Bonding on Amide 1H Chemical Shift Anisotropy Studied by Cross-Correlated Relaxation and Liquid Crystal NMR Spectroscopy

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    Site-specific (1)H chemical shift anisotropy (CSA) tensors have been derived for the well-ordered backbone amide moieties in the B3 domain of protein G (GB3). Experimental input data include residual chemical shift anisotropy (RCSA), measured in six mutants that align differently relative to the static magnetic field when dissolved in a liquid crystalline Pf1 suspension, and cross-correlated relaxation rates between the (1)H(N) CSA tensor and either the (1)H-(15)N, the (1)H-(13)C&#39;, or the (1)H-(13)C(alpha) dipolar interactions. Analyses with the assumption that the (1)H(N) CSA tensor is symmetric with respect to the peptide plane (three-parameter fit) or without this premise (five-parameter fit) yield very similar results, confirming the robustness of the experimental input data, and that, to a good approximation, one of the principal components orients orthogonal to the peptide plane. (1)H(N) CSA tensors are found to deviate strongly from axial symmetry, with the most shielded tensor component roughly parallel to the N-H vector, and the least shielded component orthogonal to the peptide plane. DFT calculations on pairs of N-methyl acetamide and acetamide in H-bonded geometries taken from the GB3 X-ray structure correlate with experimental data and indicate that H-bonding effects dominate variations in the (1)H(N) CSA. Using experimentally derived (1)H(N) CSA tensors, the optimal relaxation interference effect needed for narrowest (1)H(N) TROSY line widths is found at similar to 1200 MHz
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