Biofilm forming capacity and antibiotic susceptibility of Staphylococcus spp. with the icaA/icaD/bap genotype isolated from ocular surface of patients with diabetes

IntroductionBacterial biofilm is an exopolysaccharide matrix that is produced by bacteria while they adhere on abiotic or biotic surfaces. The bacteria living in this matrix are more resistant to antibiotics than planctonic bacteria. The biofilm formation property of the bacteria is determined by genes; and this is related to virulence of the microorganism. In ophthalmology, biofilms form especially on abiotic surfaces such as silicon tubes, contact lenses, intraocular lenses etc. AimOur aim was to investigate genotypic and phenotypic structures of biofilms that are produced by Staphylococcus spp., which was obtained from the eyes of diabetic patients and determine the effect on antibiotic susceptibility.MethodsThe study group was comprised with 83 isolates from diabetic patients and 21 isolates from non-diabetic patients. Presumptive isolates were detected and confirmed by a microbial identification system VITEK II. Automated EcoRI Ribotyping was performed. Biofilm production was detected by Congo Red Agar Plate and Microtiter Plate Assay. Disc diffusion method was used for determination of antibiotic susceptibility of isolates. ResultsOut of the 83 isolates from diabetic patients, 25 were weakly (30%), 20 were moderately (24%), and 25 were strongly (30%) biofilm positive. Seven isolates of S. aureus, 11 isolates of S.epidermidis, 2 isolates of S. warneri, 3 isolates of S.hominis, and 2 isolates of S.lugdunensis were identified as strong biofilm producers. Out of the 83 Staphylococcus isolates, 37 were cefuroxime, 18 ciprofloxacin, 11 vancomycin, 12 gatifloxacin, and 18 moxifloxacin resistant. In total, 37 strains were resistant to three or more antibiotics. There was a statistically significant relation between biofilm formation and multidrug resistance (against three or more antibiotics,p<0.001). In nondiabetic patients, 15(71%) isolates were non adherent or weakly adherent, and 2(10%) were strongly adherent biofilm positive. ConclusionIn conclusion, bacterial conjunctival flora of patients with diabetes is likely to produce biofilm. Biofilm formation is associated with multidrug rsistance in patients with diabetes

Tips for Eyelid Lacerations

I would like to point out some points that should be considered in the repair of eyelid injuries. When the patient is admitted to the hospital, the general condition of the patient is evaluated first, and then the eye should be evaluated. It is necessary to conduct a full ophthalmological examination for the condition of the eyeball and adnex

Association of Birth Parameters with Refractive Status in a Sample of Caucasian Children Aged 4–17 Years

Purpose. To investigate the association of birth parameters with refractive status in different age groups of Caucasian children. Materials and Methods. This cross-sectional study included 564 eyes of 282 children aged 4 to 17 years. All children underwent complete ophthalmologic examination. The children were divided into three groups according to their refractive status (emmetropia,myopia, and hyperopia), ages (4–7, 8-9, 10–12, and 13–17), and appropriateness for gestational age, respectively. Results. The mean age of the children was 9.2±2.8 (age range 4–17 years). The mean spheric equivalent was +0.3±1.7 (range: (−10.0)–(+10.0) diopters). The mean birth weight and gestational age were 2681.1±930.8 grams (750–5000 grams) and 37.2±3.7 weeks (25–42 weeks). According to multinominal logistic regression analysis, children with myopia were more likely to have higher birth weights than emmetropic children (OR: 1.0, 95% CI: 1.000–1.001, and P=0.028). The hypermetropes were found to be significantly small for gestational age between 13 and 17 years of age. Conclusion. Birth weight and appropriateness for gestational age as birth parameters may have an impact on development of all types of refractive errors. The hypermetropic children tended to be small for gestational age

Evaluation of the Effect of Localization of Septal Deviation on the Success of Septoplasty using NOSE Scale

Objective:To measure the effect of localization of septal deviation on the success of septoplasty using the-Nose Obstruction Symptom Evaluation (NOSE) scale in patients undergoing septoplasty and endoscopic reduction of inferior turbinates.Methods:Patients who attended Oltu Goverment Hospital ENT clinic with nasal septal deviation between March 2011 and June 2012 were included in this study. Patients who had paranasal sinus problems other than concha hypertrophy and revision cases were excluded from this study. Septal deviation was classified according to the position of the internal nasal valve as anterior, posterior or anteroposterior. All patients were examined with nasal endoscopy before and after operation and received a preoperative paranasal tomography scan. The effect of septal deviation on quality of life was evaluated using the NOSE scale preoperatively and 6 months postoperatively. All patients had endoscopic septoplasty and inferior concha reduction under general anaesthesia.Results:Ninety-seven (40 female, 57 male) patients underwent endoscopic septoplasty and concha reduction between 2011 and 2012 in Oltu Government hospital. The mean age was 29.8 years. Anterior deviation was 45, posterior deviation was 33, and anteroposterior deviation was 19. Patients with anterior deviation had a mean NOSE scale of 78.2 preoperatively and 5.8 postoperatively. Patients with posterior deviation had a mean NOSE scale of 79.2 preoperatively and 15.4 postoperatively. Patients with anteroposterior deviation had a mean NOSE scale of 82.4 preoperatively and 22.5 postoperatively (p<0.01).Conclusion:Localization of septal deviation affected postoperative septoplasty satisfaction. Patients with anteroposterior deviation were the least satisfied and patients with anterior deviation were the most satisfied after septoplasty surgery

Relation between Smartphone Use and Unilateral Ocular Pain and Headache

Ocular pain and headache may stem from many causes, ranging from infections and inflammations to radiating pain. Nowadays, use of smartphones may be an emerging cause of unilateral ocular pain and headache, especially in young population. In this study, we implemented a survey and examined the eyes of patients who used smartphones with 20/20 vision. The patients with normal neurologic and ear-nose-throat (ENT) examination findings comprised the study group. The age, duration of smartphone use, ocular examination findings, and results of ocular surface disease index (OSDI) were recorded. An association between smartphone use and ocular pain/headache was found. Spherical equivalent values of the patients with headache or ocular pain were significantly lower than those who had no pain. The OSDI scores of patients with ocular pain were significantly higher than others without ocular pain. Adverse effects of smartphone use on ocular surface and over accommodation induced by near vision may have an effect on the occurrence of ocular pain and headache

Tectonic keratoplasty simultaneous with eyelid reconstruction for cicatricial lagophthalmos

The patient, who had no social security and had keratitis in his eye, presented with corneal melting 2 weeks later. Evisceration was recommended to the patient whose light sensation was positive. With keratoplasty and lid surgery, the eye was kept in place and the vision increased to the level of hand movement

Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses

Objectives: To compare biofilm formations of two Staphylococcus epidermidis (S. epidermidis) isolates with known biofilm formation capacities on four different intraocular lenses (IOL) that have not been studied before. Materials and Methods: Two isolates obtained from ocular surfaces and identified in previous studies and stored at -86 °C in 15% glycerol in the microbiology laboratory of the Anadolu University Department of Biology were purified and used in the study. The isolates were S. epidermidis KA 15.8 (ICA+), a known biofilm producer isolate positive for icaA, icaD and bap genes, and S. epidermidis KA 14.5 (ICA-), known as a non-biofilm producer isolate negative for icaA, icaD and bap genes. The biofilm formation capacities of the 2 isolates on 4 different IOLs were compared. Two of the IOLs were acrylic (UD613 [IOL A], Turkey; SA60AT [IOL B], USA), and the other two were polymethyl methacrylate (PMMA) (B60130C [IOL C], India; B55125C [IOL D], India). Bacterial enumeration and optical density measurements were done from biofilms that formed on the IOLs. Biofilms were imaged using scanning electron microscopy. Results: Mean bacterial counts on the IOLs were 7.1±0.4 log10 CFU/mL with the ICA+ isolate, and 6.7±0.8 log10 CFU/mL with the ICA- isolate; there were no statistically significant differences. Biofilm formation was lower with acrylic lenses than PMMA lenses with both isolates (p=0.009 and p=0.013). The highest biofilm production was obtained on IOL C (PMMA) (p<0.001) and the lowest was obtained on IOL A (hydrophilic acrylic) (p<0.001). Conclusion: Bacterial counts after biofilm formation were lower on acrylic lenses, especially hydrophilic acrylic with hydrophobic properties. Further animal and in vivo studies are required to support the findings of this study

Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses

Objectives: To compare biofilm formations of two Staphylococcus epidermidis (S. epidermidis) isolates with known biofilm formation capacities on four different intraocular lenses (IOL) that have not been studied before. Materials and Methods: Two isolates obtained from ocular surfaces and identified in previous studies and stored at -86 °C in 15% glycerol in the microbiology laboratory of the Anadolu University Department of Biology were purified and used in the study. The isolates were S. epidermidis KA 15.8 (ICA+), a known biofilm producer isolate positive for icaA, icaD and bap genes, and S. epidermidis KA 14.5 (ICA-), known as a non-biofilm producer isolate negative for icaA, icaD and bap genes. The biofilm formation capacities of the 2 isolates on 4 different IOLs were compared. Two of the IOLs were acrylic (UD613 [IOL A], Turkey; SA60AT [IOL B], USA), and the other two were polymethyl methacrylate (PMMA) (B60130C [IOL C], India; B55125C [IOL D], India). Bacterial enumeration and optical density measurements were done from biofilms that formed on the IOLs. Biofilms were imaged using scanning electron microscopy. Results: Mean bacterial counts on the IOLs were 7.1±0.4 log10 CFU/mL with the ICA+ isolate, and 6.7±0.8 log10 CFU/mL with the ICA- isolate; there were no statistically significant differences. Biofilm formation was lower with acrylic lenses than PMMA lenses with both isolates (p=0.009 and p=0.013). The highest biofilm production was obtained on IOL C (PMMA) (p<0.001) and the lowest was obtained on IOL A (hydrophilic acrylic) (p<0.001). Conclusion: Bacterial counts after biofilm formation were lower on acrylic lenses, especially hydrophilic acrylic with hydrophobic properties. Further animal and in vivo studies are required to support the findings of this study

Demographic Characteristics and Clinical Outcome of Work-related Open Globe Injuries in the Most Industrialised Region of Turkey

Objectives: To evaluate demographic characteristics and clinical outcomes of work-related open globe injuries in the most industrialized region of Turkey. Materials and Methods: The demographic and medical records of patients with work-related open globe injuries who presented to the ophthalmology or emergency departments with an official occupational accident report were retrospectively reviewed. Visual acuity categories were defined according to the World Health Organization. The injury types and zones of the open globes were classified according to Birmingham Eye Trauma Terminology System. Results: Among 479 patients with work-related eye injuries in 5 years, there were 102 eyes of 101 patients with open globe injuries (21%). The mean age of the patients was 34.5±8.9 years with a mean follow-up of 12.5±12.6 months. The injuries peaked in June in the hour between 12:00 and 13:00. Eighty-six percent presented to emergency services within 12 hours after the injury. Twenty-two percent of the patients had been wearing protective eyewear at the time of injury. The open globe injuries were penetrating in 51%, intraocular foreign body in 40%, rupture in 7% and perforation in 2% of the eyes. The most frequent finding was traumatic cataract. Final visual acuity of 33.3% of patients was below 3/60. Seventy-eight percent of patients that had visual acuity worse than 6/18 at presentation had visual acuity of 6/18 or better at final visit. Sixty-three percent of eyes which had injuries involving all 3 zones resulted in phthisis bulbi, enucleation or evisceration. Conclusion: Work-related open globe injuries may have severe consequences such as visual impairment and blindness among the young male working population in industrialized areas. Nearly half of the occupational open globe injuries resulted in visual impairment and blindness