14 research outputs found

    Solutions in generalised linear systems via Maple

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    In this thesis we consider several distinct problems in linear systems theory and encompass the implementation of such work via the symbolic computational language Maple. Our analytical contribution is split into three main areas these being: the solution to a regular, discrete-time ARMA-representation; the computation of the generalised inverse of a rational matrix P(s)ER(s)n×m; and the computation of the invariant direction vectors associated with a regular polynomial matrix description (PMD). [Continues.

    Structure and function of plant disease resistance genes

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    <div>Lecture given by Doctor Jonathan Jones, Sainsbury Laboratory, John Innes Centre, England, UK at the Waite Campus, University of Adelaide, 30.1.1996</div><div><br></div><p>This lecture is part of the Campus Seminars and Distinguished Lecturer Series, Waite Campus, University of Adelaide, 1991 – 1997. </p> <a href="https://figshare.com/projects/Campus_Seminars_and_Distinguished_Lecturer_Series_Waite_Campus_University_of_Adelaide/17756">https://figshare.com/projects/Campus_Seminars_and_Distinguished_Lecturer_Series_Waite_Campus_University_of_Adelaide/17756</a

    Does Service FDI Locate Differently to Manufacturing FDI? A Regional Analysis for Great Britain

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    <p>Jones J. and Wren C. Does service FDI locate differently to manufacturing FDI? A regional analysis for Great Britain, <i>Regional Studies</i>. This paper analyses the location of foreign direct investment (FDI) in services at the NUTS-2 region level for Great Britain over 1996–2005, covering the period of growth in FDI. It finds strong differences in service FDI location compared with manufacturing FDI. Unlike manufacturing, service FDI location is determined at no higher than the NUTS-2 region level, while it is more dynamic and converging to the larger markets. A similar pattern is found for the services that are most forward-linked to manufacturing industries. The paper concludes that access to final consumer demand is important for service FDI, with implications for UK regional policy.</p

    Volume Fraction of Ether Is a Significant Factor in Controlling Conductivity in Proton Conducting Polyether Based Polymer Sol–Gel Electrolytes

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    We have synthesized several copolymers of methyl polyethylene glycol siloxane (MePEG<sub>7</sub>SiO<sub>3</sub>)<sub><i>m</i></sub> and methyl polypropylene glycol siloxane (MePPG<sub><i>n</i></sub>SiO<sub>3</sub>)<sub><i>m</i></sub> as hydrogen ion (H<sup>+</sup>) conducting polymer electrolytes. These copolymers were prepared by a sol–gel polymerization of mixtures of the MePEG and MePPG monomers. We synthesized these H<sup>+</sup> conducting polymer electrolytes in order to study the relationship between observed ionic conductivity and structural properties such as viscosity, fractional free volume, and volume fraction of ether. We found that viscosity increased as the fraction of the smaller comonomer increased. For the MePPG<sub>2</sub>/MePPG<sub>3</sub> copolymer, an increase in fractional free volume increased the fluidity. The heterogeneous copolymers (PEG/PPG copolymers) obeyed the Doolittle equation, while the homogeneous (PEG/PEG and PPG/PPG) copolymers did not. The increase of FFV did not, however, correspond to an increase in conductivity, as would have been predicted by the Forsythe equation. The conductivity data did correspond to a modified Forsythe equation substituting Volume Fraction of Ether (V<sub>f,ether</sub>) for FFV. We conclude that the proton conductivity of MePEG copolymers is more dependent on the volume fraction of ether than on the fractional free volume

    Quantitative analysis of trophic factors BDNF, NT3 and NT4 in mesenchymal stem cells and MSC-conditioned medium.

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    <p>A) Quantitative PCR analysis of trophic factors released in the conditioned medium of mesenchymal stem cells isolated from wildtype (WT) and Friedreich’s ataxia mice (AtF) under normal culture conditions. B) Quantitative PCR analysis of trophic factors released in the conditioned medium of mesenchymal stem cells isolated from wildtype (MSC-WT) and Friedreich’s ataxia mice (MSC-AtF), under normal culture conditions (CT) and exposed for 24 hours to hydrogen peroxide (H2O2). N = 6, *p<0.001. C) Western blot analysis of the trophic factors in mesenchymal stem cell-conditioned medium under normal culture conditions and exposed to hydrogen peroxide. P indicates Ponceau S staining as loading control. To the right, gel density analysis of the western blots, where hydrogen peroxide treated stem cells (MSC-WT H2O2 and MSC-AtF H2O2) were compared to their respective control culture condition. N = 4 in all cases, *p<0.05, **p<0.01. D) Quantitative PCR analysis of oxidative stress-related genes, as well as frataxin, of dorsal root ganglia cultures exposed to oxidative stress (CT H2O2) as well as with the stem cell conditioned medium of wildtype (MSC-WT H2O2 K252) and YG8 (MSC ATF H2O2 K252) mice.</p

    Dorsal root ganglia cultures exposed to hydrogen peroxide.

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    <p>(A–D) Dorsal root ganglia primary cultures before (A), 24 hours after exposure to hydrogen peroxide (B), and 24 hours after exposure to hydrogen peroxide and cultured with conditioned medium from wildtype and YG8 stem cells (C and D, respectively). E) The histogram depicts the percentage of live cells in culture among the different culture conditions, considering the control culture as 100%. After being submitted to oxidative stress (labeled H2O2 in the histogram), over 67% of the cells in culture die. However, almost all cells survive when exposed to hydrogen peroxide and under mesenchymal stem cell-conditioned medium. F–I) Immunocytochemistry of Tuj1 (in red) and GFAP (in green) of the dorsal root ganglia cultures. J–K) The histograms present the percentage of cells positive for Tuj1 and GFAP, respectively, in the different cell cultures. The percentages are with respect to the control, normalized to 100%. L) Western blot analysis of Tuj1 and GFAP in the various culture conditions, with beta-actin as control. N = 3 in all cases, *p<0.05, **p<0.01.</p

    Quantitative analysis of dorsal root ganglia cells.

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    <p>A) Quantitative PCR analysis of dorsal root ganglia cells of wildtype (WT) versus YG8 (AtF) mice under normal culture conditions. B) Quantitative PCR analysis of dorsal root ganglia cells under normal culture medium (Control), exposed to hydrogen peroxide (H2O2) and exposed to hydrogen peroxide with conditioned medium from wildtype and YG8 stem cells (MSC-WT and MSC-AtF, respectively). H2O2 cultures express high Bax levels, indicating apoptosis of surviving cells. DRG cultured with conditioned medium presented a significant increase in the expression levels of frataxin, oxidative stress markers, as well as high Bcl-2 and low Bax levels. N = 6 in all cases except for MSC-AtF where n = 4, *p<0.05, **p<0.01, ***p<0.001. C) Western blot analysis of frataxin in the various culture conditions (n = 4). Two bands were observed, one at 30 kDa corresponding to the precursor form of frataxin, and one at 18 kDa corresponding to the mature form.</p

    Use of mobilization and felaxation techniques in patients with periarthritis humeroscapulatis

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    T. aestivum fusion validation by RNA-seq. Summary of manual validation of T. aestivum NLR-IDs using RNA-seq data. (XLSX 60 kb

    Additional file 8: of Comparative analysis of plant immune receptor architectures uncovers host proteins likely targeted by pathogens

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    Manual verification of domains predicted by high-throughput scripts with webservers for brassica, tomato, wheat and soybean. (XLSX 22 kb

    The History of the Botanic Garden in Smíchov in Light of Archival Sources

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    (in English) Botanical Garden in Smíchov formed one of the foundations for the study of medicine, chemistry, and other natural sciences in our territoryfrom the second half of the 18thcentury to the late 20thcentury. The aim of the study is to demonstrate the possibilities of using archival sources not only for the study of the history of botany, but also for finding and locating objects of the garden on historic maps and plans.The work also combines multiple disciplines and also offers new knowledge about the history of botany, postal and architecture
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