amilFP597 promoter

Analysis of the amilFP597 promoter copy ratios in six A. millepora morphs with different levels of redness

Sequences RFP full length genes

All raw sequences used to reconstruct the amilFP597 gene in A. millepora.Full-length sequences of indel (-) and indel (+) promoter variants of the amilFP597 gene were obtained for the MR morph. These sequences, extending from the promoter region to the 3’UTR, were produced by joining the sequences of two overlapping PCR products covering the 5’ region [promoter to exon 3 amplified using primers pRFPlargeF (+) or pRFPsmallF (-) and RFP SP1] and the 3’ region (intron 2 to 3’UTR amplified using primers RFP_I2-3’U_F and RFP_I2-3’U_R2). Sequence differences in the overlap between the 5’ and 3’ region fragments were used to assign the latter to either the indel (+) or indel (-) promoter variant genes. The promoter-exon 3 and intron 2-3’UTR fragments were cloned and sequenced on both strands using vector primers and by primer walking. The assembled sequences have been submitted to GenBank as accessions KC818413 [indel (-) gene] and KC818414 [indel (+) gene]

amilFP597 exon 3 sequences

The chromophore coding sequences of RFP-related genes of A. millepora were amplified using genomic DNA of LR, MR and HR morphs as the template with primers designed to conserved regions within exon 3

Abs spectra of A millepora

Absorption spectra of clarified tissue extracts of the HR and LR A. millepora morphs. The absorption spectrum of purified recombinant amilFP597 normalised to the peak value of the HR spectrum is included for comparison

Spectroscopic characteristics of GFP-like proteins

All spectroscopic data utilised for the characterisation of amilFP597, amilCP506, amilCP564 and amilFP60

amilFP597 copies

Copy numbers of amilFP597 variant genes determined by semi-quantitative PCR amplification of a conserved genomic exon 3 fragment and by diagnostic restriction analysis with ApeK1