A Model for Collaborative Filtering Recommendation in E-Commerce Environment

In modern business environment, product life cycle gets shorter and the customer’s buying preference changes over time. Time plays a more and more important role in collaborative filtering. However, there is a gap in one class collaborative filtering (OCCF). On the basis of collecting different real-time information, this paper proposes an optimization model for e-retailers. Through comparing different methods with different weights, results show that real-time dependent in OCCF performs better in improving the quality of recommendation. The model is effective in cross-selling e-commerce, personalized, targeted recommendation sales

Meta-analysis of the effects of 1-methylcyclopropene (1-MCP) treatment on climacteric fruit ripening

1-Methylcyclopropene (1-MCP) is an inhibitor of ethylene perception that is widely used to maintain the quality of several climacteric fruits during storage. A large body of literature now exists on the effects of 1-MCP on climacteric fruit ripening for different species and environmental conditions, presenting an opportunity to use meta-analysis to systematically dissect these effects. We classified 44 ripening indicators of climacteric fruits into five categories: physiology and biochemistry, quality, enzyme activity, color, and volatiles. Meta-analysis showed that 1-MCP treatment reduced 20 of the 44 indicators by a minimum of 22% and increased 6 indicators by at least 20%. These effects were associated with positive effects on delaying ripening and maintaining quality. Of the seven moderating variables, species, 1-MCP concentration, storage temperature and time had substantial impacts on the responses of fruit to 1-MCP treatment. Fruits from different species varied in their responses to 1-MCP, with the most pronounced responses observed in rosaceous fruits, especially apple, European pear fruits, and tropical fruits. The effect of gaseous 1-MCP was optimal at 1 μl/l, with a treatment time of 12–24 h, when the storage temperature was 0 °C for temperate fruits or 20 °C for tropical fruits, and when the shelf temperature was 20 °C, reflecting the majority of experimental approaches. These findings will help improve the efficacy of 1-MCP application during the storage of climacteric fruits, reduce fruit quality losses and increase commercial value

Translating dosage compensation to trisomy 21

Down syndrome is the leading genetic cause of intellectual disabilities, occurring in 1 out of 700 live births. Given that Down syndrome is caused by an extra copy of chromosome 21 that involves over-expression of 400 genes across a whole chromosome, it precludes any possibility of a genetic therapy. Our lab has long studied the natural dosage compensation mechanism for X chromosome inactivation. To “dosage compensate” X-linked genes between females and males, the X-linked XIST gene produces a large non-coding RNA that silences one of the two X chromosomes in female cells. The initial motivation of this study was to translate the natural mechanisms of X chromosome inactivation into chromosome therapy for Down syndrome. Using genome editing with zinc finger nucleases, we have successfully inserted a large XIST transgene into Chromosome 21 in Down syndrome iPS cells, which results in chromosome-wide transcriptional silencing of the extra Chromosome 21. Remarkably, deficits in proliferation and neural growth are rapidly reversed upon silencing one chromosome 21. Successful trisomy silencing in vitro surmounts the major first step towards potential development of “chromosome therapy” for Down syndrome. The human iPSC-based trisomy correction system we established opens a unique opportunity to identify therapeutic targets and study transplantation therapies for Down syndrome

Identification of Important Physiological Traits and Moderators That Are Associated with Improved Salt Tolerance in CBL and CIPK Overexpressors through a Meta-Analysis

The CBL-CIPK pathway is a plant-specific Ca2+ sensor relaying pathway that has been shown to be involved in plant response to salt stress. Over-expression of CBL-CIPK network genes has been reported to increase salt tolerance in many studies. The studies on the overexpression of CBL-CIPK network genes, however, have used various indices to evaluate the effect of these genes on salt tolerance and have indicated a variety of roles for the major CBL-CIPK pathway genes. Therefore, it is of great interest to analyze the various effects resulting from the overexpression CBL-CIPK pathway genes and their relation to salt tolerance. The meta-analysis conducted in the present study investigated how over-expression of CBLs or CIPKs in transgenic plants affects the response to salt stress and identified the increase or decrease that occurs in these experimental variables when foreign CIPK or CBL genes are overexpressed in transgenic plants. The data from the collective studies on over-expression of CIPKs indicated that 6 of the 11 examined parameters (main effects) increased by 22% or more, while two of the six examined parameters increased by at least 78% in transgenic plants overexpressing CBL genes. In addition to analyzing the impact of overexpression on the main effects, eight different modifying parameters were also analyzed. Results indicated that several moderators impact the extent to which overexpression of CBLs and CIPKs affect the main parameters. The majority of CBLs have been obtained from dicotyledonous plants and most of the CBLs and CIPKs have been expressed in dicotyledonous plants. In comparison to homologous expression, the meta-analysis indicated that heterogeneous expression of CBLs resulted in greater increases in seed germination. The results of the meta-analysis provide information that could be useful in designing research to examine the mechanisms by which CBL-CIPK pathway genes increase salt tolerance in plants

Solvent-controlled plasmon-assisted surface catalysis reaction of 4-aminothiophenol dimerizing to p,p'-dimercaptoazobenzene on Ag nanoparticles

A large number of literatures have investigated the selective photocatalytic reaction of 4-aminothiophenol (PATP) to p,p'-dimercaptoazobenzene (DMAB). Most of them mainly study the contribution of substrate, excitation wavelength, exposure time, pH and added cations to plasmon-assisted surface catalytic reactions. However, we mainly study focuses on the effects of solvents on the dimerization of PATP to DMAB under the action of Ag nanoparticles (NPs). In experiments, a variety of diols was selected as solvents for the probe molecule PATP, and power-dependent SERS spectra were obtained at an excitation wavelength of 532 nm. From the laser-dependent SERS spectrum, we found that the characteristic peak enhancement effect of the product DMAB in different solvents is significantly different. That is, different solvents could regulate the rate at which DMAB is produced from PATP. Based on the experimental results, we further explored how different diol solvents regulate the response of PATP to DMAB. Our conclusion is that the solvent in the system can quickly capture the hot electrons generated by the decay of the plasmon, so that the remaining holes can oxidize PATP to form DMAB. The ability to trap hot electrons is different due to the difference in the position of the functional groups in the solvent, so that the photocatalytic reaction rate of the hole-oxidized PATP is different. The ability to capture electrons varies depending on the position of the functional groups in the solvent, so the oxidation rate of the photocatalytic reaction is also different. This work not only deepens our understanding of the mechanism of hole-driven surface catalysis oxidation reaction, but also provides a convenient method for regulating the rate of catalytic oxidation

Stable Colonization of Orally Administered Lactobacillus casei SY13 Alters the Gut Microbiota

The gut microbiota plays an important role in intestinal health. Probiotics such as Lactobacillus are known to regulate gut microbes and prevent diseases. However, most of them are unable to colonize their stability in hosts’ intestinal tracts. In this study, we investigated the ability of Lactobacillus casei SY13 (SY13) to colonize the intestinal tract of BALB/c mice, after its oral administration for a short-term (once for a day) and long-term (once daily for 27 days) duration. Furthermore, we also evaluated the influence of its administration on the gut microbial structure and diversity in mice. Male BALB/c mice were gavaged with 108 colony-forming units (CFU) of SY13, and TaqMan-MGB probe and Illumina MiSeq sequencing were performed to assess the colonization ability and bacterial community structure in the cecum contents. The results showed that long-term treatment with SY13 enhanced its ability to form a colony in the intestine tract in contrast to the short-term treatment group, whose colony was retained for only 3 days. Oral administration of SY13 also significantly enhanced the gut microbial diversity. Short-term treatment with SY13 (SSY13) elevated Firmicutes and diminished Bacteroidetes phyla compared with long-term treatment (LSY13) and controls. The findings laid the foundation for the study of probiotic colonization ability and improvement of microbiota for the prevention of gut diseases

Potential mechanisms of osthole against bladder cancer cells based on network pharmacology, molecular docking, and experimental validation

Abstract Background Osthole was traditionally used in treatment for various diseases. However, few studies had demonstrated that osthole could suppress bladder cancer cells and its mechanism was unclear. Therefore, we performed a research to explore the potential mechanism for osthole against bladder cancer. Methods Internet web servers SwissTargetPrediction, PharmMapper, SuperPRED, and TargetNet were used to predict the Osthole targets. GeneCards and the OMIM database were used to indicate bladder cancer targets. The intersection of two target gene fragments was used to obtain the key target genes. Protein–protein interaction (PPI) analysis was performed using the Search Tool for the Retrieval of Interacting Genes (STRING) database. Furthermore, we used gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses to explore the molecular function of target genes. AutoDock software was then used to perform molecular docking of target genes,osthole and co-crystal ligand. Finally, an in vitro experiment was conducted to validate bladder cancer inhibition by osthole. Results Our analysis identified 369 intersection genes for osthole, the top ten target genes included MAPK1 , AKT1, SRC, HRAS, HASP90AA1, PIK3R1, PTPN11, MAPK14 , CREBBP, and RXRA. The GO and KEGG pathway enrichment results revealed that the PI3K-AKT pathway was closely correlated with osthole against bladder cancer. The osthole had cytotoxic effect on bladder cancer cells according to the cytotoxic assay. Additionally, osthole blocked the bladder cancer epithelial-mesenchymal transition and promoted bladder cancer cell apoptosis by inhibiting the PI3K-AKT and Janus kinase/signal transducer and activator of transcription (JAK/STAT3) pathways. Conclusions We found that osthole had cytotoxic effect on bladder cancer cells and inhibited invasion, migration, and epithelial-mesenchymal transition by inhibiting PI3K-AKT and JAK/STAT3 pathways in in vitro experiment. Above all, osthole might have potential significance in treatment of bladder cancer. Subjects Bioinformatics, Computational Biology, Molecular Biology

Image_1_Identification of a six-gene prognostic signature for bladder cancer associated macrophage.tif

As major components of the tumor microenvironment (TME), tumor-associated macrophages (TAMs) play an exceedingly complicated role in tumor progression and tumorigenesis. However, few studies have reported the specific TAM gene signature in bladder cancer. Herein, this study focused on developing a TAM-related prognostic model in bladder cancer patients based on The Cancer Genome Atlas (TCGA) data. Weighted Gene Co-Expression Network Analysis (WGCNA) was used to identify key genes related to TAM (M2 macrophage). Gene ontology (GO) enrichment and the Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway analysis showed the functional categories of the key genes. Simultaneously, we used the Least Absolute Shrinkage and Selection Operator (LASSO) and univariate and multivariate Cox regressions to establish a TMA-related prognostic model containing six key genes: TBXAS1, GYPC, HPGDS, GAB3, ADORA3, and FOLR2. Subsequently, single-cell sequencing data downloaded from Gene Expression Omnibus (GEO) suggested that the six genes in the prognostic model were expressed in TAM specifically and may be involved in TAM polarization. In summary, our research uncovered six-TAM related genes that may have an effect on risk stratification in bladder cancer patients and could be regarded as potential TAM-related biomarkers.</p

Image_4_Identification of a six-gene prognostic signature for bladder cancer associated macrophage.tif

As major components of the tumor microenvironment (TME), tumor-associated macrophages (TAMs) play an exceedingly complicated role in tumor progression and tumorigenesis. However, few studies have reported the specific TAM gene signature in bladder cancer. Herein, this study focused on developing a TAM-related prognostic model in bladder cancer patients based on The Cancer Genome Atlas (TCGA) data. Weighted Gene Co-Expression Network Analysis (WGCNA) was used to identify key genes related to TAM (M2 macrophage). Gene ontology (GO) enrichment and the Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway analysis showed the functional categories of the key genes. Simultaneously, we used the Least Absolute Shrinkage and Selection Operator (LASSO) and univariate and multivariate Cox regressions to establish a TMA-related prognostic model containing six key genes: TBXAS1, GYPC, HPGDS, GAB3, ADORA3, and FOLR2. Subsequently, single-cell sequencing data downloaded from Gene Expression Omnibus (GEO) suggested that the six genes in the prognostic model were expressed in TAM specifically and may be involved in TAM polarization. In summary, our research uncovered six-TAM related genes that may have an effect on risk stratification in bladder cancer patients and could be regarded as potential TAM-related biomarkers.</p