MOESM1 of A Pseudomonas putida efflux pump acts on short-chain alcohols

Additional file 1: Figure S1. Plasmid stability of pBbB8k-TtgABC in Pseudomonas putida DOT-T1E. The plasmid-carrying strain was grown for 5 consecutive days with 2 mM L-arabinose for inducing expression of TtgABC and without kanamycin. The medium was renewed once per day. Plasmid stability was determined by plating on kanamycin plates and comparison of viable cell numbers

MOESM2 of A Pseudomonas putida efflux pump acts on short-chain alcohols

Additional file 2: Figure S2. Cell-level GFP expression using the L-arabinose inducible PBAD promoter in Pseudomonas putida DOT-T1E. Cells carrying the pBbB8k-GFP plasmid were induced overnight without (brown), with 1 mM (red), 10 mM (purple), and 100 mM (green) L-arabinose. Cell-level fluorescence of GFP was measured using flow cytometry. The distributions indicate a homologous and quantitative increase of expression at the cell level

MOESM3 of A Pseudomonas putida efflux pump acts on short-chain alcohols

Additional file 3: Figure S3. TtgABC expression does not increase growth of P. putida DOT-T1E in n-butanol. Growth of P. putida DOT-T1E without plasmid (WT), without induction (ttgABC - ), and with induction (ttgABC + ) in 0% (a) and 0.5% (b) n-butanol in 15 mL conical tubes containing 3 mL medium. Similar to the results obtained from the plate reader (cf. Fig. 3), growth of the wild-type strain is slightly faster with and without n-butanol, and TtgABC expression does not increase growth in n-butanol

MOESM4 of A Pseudomonas putida efflux pump acts on short-chain alcohols

Additional file 4. Quantification of extracellular n-butanol using GC-FID. The spreadsheet contains the estimated fraction of viable cells after incubation in PBS containing 0.2 and 1% n-butanol, and the measurement values of quantified extracellular n-butanol in induced (ttgABC + ) and non-induced strains (ttgABC - ). The original readings for n-butanol and the internal standard isoprenol are shown, as well as the n-butanol measurements normalized by the standard, and the derived concentrations as volume per volume and milligrams per milliliter. The inlay shows the n-butanol standard curve, indicating that concentrations are in a linear measurement range

Development of Supporting Software for Driving School

This thesis is focused on design and implementation web application it based on the requirements and analysis of the current situation using technologies PHP and MySQL. It is specification system for planning rides, final exams and related functions in driving school

The Fine Line Employers Walk: Is It a Justified Business Practice, or Discrimination?

Additional file 3: Figure S3. Dynamics of 13C-labeling pulse experiment for WH04

MOESM4 of Examining Escherichia coli glycolytic pathways, catabolite repression, and metabolite channeling using Δpfk mutants

Additional file 4: Figure S4. Kinetics of 13C-isotopic incorporation in ∆pfkA (JW3887) culture taken immediately after 13C6-glucose pulse

MOESM1 of Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria

Additional file 1. Additional data and note for the engineered cyanobacteria strains

Additional file 1: of CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae

Figure S1. Showing efficiency of single step integration of the beta-carotenoid pathway in different strain backgrounds. Additionally, the file includes supplementary methods. Table S1. Efficiency of targeted integration using CrEdit. Table S2. List of strains used. Table S3. List of plasmids used. Table S4. gRNA sequences. Table S5. DNA and BioBricks and gBlocks. Table S6. Primers used in this study