120 research outputs found
NMR structure of the human prion protein with the pathological Q212P mutation reveals unique structural features.
One-pot efficient synthesis of N ι-urethane-protected β- and γ-amino acids
1-[(4-Methylphenyl)oxy]pyrrolidine-2,5-dione and 1-[(4-methylphenyl)oxy]piperidine-2,6-dione react in a Lossen-type reaction with primary alcohols in the presence of triethylamine to furnish corresponding N(Îą)-urethane-protected β-alanine and Îł-aminopropionic acid (GABA), respectively, with excellent yields and purities, in an essentially âone-potâ procedure
The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases
Hydrogen peroxide is an important regulator of protein tyrosine phosphatase activity via reversible oxidation. However, the role of iron in this reaction has not been yet elucidated. Here we compare the influence of hydrogen peroxide and the ferrous iron (reagent for Fenton reaction) on the enzymatic activity of recombinant CD45, LAR, PTP1B phosphatases and cellular CD45 in Jurkat cells. The obtained results show that ferrous iron (II) is potent inhibitor of CD45, LAR and PTP1B, but the inhibitory effect is concentration dependent. We found that the higher concentrations of ferrous iron (II) increase the inactivation of CD45, LAR and PTP1B phosphatase caused by hydrogen peroxide, but the addition of the physiological concentration (500 nM) of ferrous iron (II) has even a slightly preventive effect on the phosphatase activity against hydrogen peroxide
Fast evaluation of protein dynamics from deficient 15N relaxation data
Simple and convenient method of protein dynamics evaluation from the insufficient experimental 15N relaxation data is
presented basing on the ratios, products, and differences of longitudinal and transverse 15N relaxation rates obtained at a single magnetic field. Firstly, the proposed approach allows evaluating overall tumbling correlation time (nanosecond time scale). Next, local parameters of the model-free approach characterizing local mobility of backbone amide NâH vectors on two different time scales, S2 and Rex, can be elucidated. The generalized order parameter, S2, describes motions on the time scale faster than the overall tumbling correlation time (pico- to nanoseconds), while the chemical exchange term, Rex, identifies processes slower than the overall tumbling correlation time (micro- to milliseconds). Advantages and disadvantages of different methods of data handling are thoroughly discussed
NMR as a âgold standardâ method in drug design and discovery
Studying disease models at the molecular level is vital for drug development in order to improve treatment and prevent a wide range of human pathologies. Microbial infections are still a major challenge because pathogens rapidly and continually evolve developing drug resistance. Cancer cells also change genetically, and current therapeutic techniques may be (or may become) ineffective in many cases. The pathology of many neurological diseases remains an enigma, and the exact etiology and underlying mechanisms are still largely unknown. Viral infections spread and develop much more quickly than does the corresponding research needed to prevent and combat these infections; the present and most relevant outbreak of SARS-CoV-2, which originated in Wuhan, China, illustrates the critical and immediate need to improve drug design and development techniques. Modern day drug discovery is a time-consuming, expensive process. Each new drug takes in excess of 10 years to develop and costs on average more than a billion US dollars. This demonstrates the need of a complete redesign or novel strategies. Nuclear Magnetic Resonance (NMR) has played a critical role in drug discovery ever since its introduction several decades ago. In just three decades, NMR has become a âgold standardâ platform technology in medical and pharmacology studies. In this review, we present the major applications of NMR spectroscopy in medical drug discovery and development. The basic concepts, theories, and applications of the most commonly used NMR techniques are presented. We also summarize the advantages and limitations of the primary NMR methods in drug development
Undercover Toxic MĂŠnage Ă Trois of Amylin, Copper (II) and Metformin in Human Embryonic Kidney Cells
In recent decades, type 2 diabetes complications have been correlated with amylin aggregation,
copper homeostasis and metformin side effects. However, each factor was analyzed
separately, and only in some rare cases copper/amylin or copper/metformin complexes were
considered. We demonstrate for the first time that binary metformin/amylin and tertiary copper
(II)/amylin/metformin complexes of high cellular toxicity are formed and lead to the formation
of aggregated multi-level lamellar structures on the cell membrane. Considering the increased
concentration of amylin, copper (II) and metformin in kidneys of T2DM patients, our findings on the
toxicity of amylin and its adducts may be correlated with diabetic nephropathy development
Metal complex formation and anticancer activity of cu(I) and cu(ii) complexes with metformin
Metformin has been used for decades in millions of type 2 diabetes mellitus patients. In this
time, correlations between metformin use and the occurrence of other disorders have been noted, as
well as unpredictable metformin side effects. Diabetes is a significant cancer risk factor, but unexpectedly,
metformin-treated diabetic patients have lower cancer incidence. Here, we show that metformin
forms stable complexes with copper (II) ions. Both copper(I)/metformin and copper(II)/metformin
complexes form adducts with glutathione, the main intracellular antioxidative peptide, found at
high levels in cancer cells. Metformin reduces cell number and viability in SW1222 and K562 cells,
as well as in K562-200 multidrug-resistant cells. Notably, the antiproliferative effect of metformin is
enhanced in the presence of copper ions
What doesn't kill you makes you stronger: Future applications of amyloid aggregates in biomedicine
Amyloid proteins are linked to the pathogenesis of several diseases including Alzheimerâs
disease, but at the same time a range of functional amyloids are physiologically important in humans.
Although the disease pathogenies have been associated with protein aggregation, the mechanisms
and factors that lead to protein aggregation are not completely understood. Paradoxically, unique
characteristics of amyloids provide new opportunities for engineering innovative materials with
biomedical applications. In this review, we discuss not only outstanding advances in biomedical
applications of amyloid peptides, but also the mechanism of amyloid aggregation, factors aecting
the process, and core sequences driving the aggregation. We aim with this review to provide a useful
manual for those who engineer amyloids for innovative medicine solutions
New Advances in Fast Methods of 2D NMR Experiments
Although nuclear magnetic resonance spectroscopy is a potent analytical tool for identification, quantification, and structural elucidation, it suffers from inherently low sensitivity limitations. This chapter focuses on recently reported methods that enable quick acquisition of NMR spectra, as well as new methods of faster, efficient, and informative two-dimensional (2D) NMR methods. Fast and efficient data acquisition has risen in response to an increasing need to investigate chemical and biological processes in real time. Several new techniques have been successfully introduced. One example of this is band-selective optimized-flip-angle short-transient (SOFAST) NMR, which has opened the door to studying the kinetics of biological processes such as the phosphorylation of proteins. The fast recording of NMR spectra allows researchers to investigate time sensitive molecules that have limited stability under experimental conditions. The increasing awareness that molecular structures are dynamic, rather than static, has pushed some researchers to find alternatives to standard, time-consuming methods of 15N relaxation observables acquisition
The Best Peptidomimetic Strategies to Undercover Antibacterial Peptides
Health-care systems that develop rapidly and efficiently may increase the lifespan of humans. Nevertheless, the older population is more fragile, and is at an increased risk of disease development. A concurrently growing number of surgeries and transplantations have caused antibiotics to be used much more frequently, and for much longer periods of time, which in turn increases microbial resistance. In 1945, Fleming warned against the abuse of antibiotics in his Nobel lecture: âThe time may come when penicillin can be bought by anyone in the shops. Then there is the danger that the ignorant man may easily underdose himself and by exposing his microbes to non-lethal quantities of the drug make them resistantâ. After 70 years, we are witnessing the fulfilment of Flemingâs prophecy, as more than 700,000 people die each year due to drug-resistant diseases. Naturally occurring antimicrobial peptides protect all living matter against bacteria, and now different peptidomimetic strategies to engineer innovative antibiotics are being developed to defend humans against bacterial infections
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