Neutron spectrum determination of p+Be reaction for 30 MeV protons using the multi-foil activation technique

At the NPI in Rez, the p + Be source reaction was investigated for 30 MeV proton beam and thick beryllium target. For neutron field determination of the p(30)+Be source reaction in close source-to-sample distance, the multi-foil activation technique with a set of 10 activation materials (Au, Co, Lu, Ti, In, Al, Y, Fe, Ni, Nb) was utilized. From resulting reaction rates, the neutron spectrum was reconstructed using the SAND-II unfolding code. New neutron field of white spectrum up to 28 MeV has an intensity of 8.6 × 1010 cm−2s−1 close to target. The obtained neutron field extends the utilization of cyclotron-based fast neutron sources at the NPI and provides new experimental opportunities for future irradiation experiments such as fast neutron activation analysis, nuclear data validation, and radiation damage study of electronics and materials for nuclear energetics

Neutron spectrum determination of p+Be reaction for 30 MeV protons using the multi-foil activation technique

At the NPI in Rez, the p + Be source reaction was investigated for 30 MeV proton beam and thick beryllium target. For neutron field determination of the p(30)+Be source reaction in close source-to-sample distance, the multi-foil activation technique with a set of 10 activation materials (Au, Co, Lu, Ti, In, Al, Y, Fe, Ni, Nb) was utilized. From resulting reaction rates, the neutron spectrum was reconstructed using the SAND-II unfolding code. New neutron field of white spectrum up to 28 MeV has an intensity of 8.6 × 1010 cm−2s−1 close to target. The obtained neutron field extends the utilization of cyclotron-based fast neutron sources at the NPI and provides new experimental opportunities for future irradiation experiments such as fast neutron activation analysis, nuclear data validation, and radiation damage study of electronics and materials for nuclear energetics

Resolving power of pixel detector Timepix for wide-range electron, proton and ion detection

The resolving power of the Timepix detector for wide-range charged particle detection has been examined and evaluated in defined radiation fields. The goal is to broadly characterize mixed-radiation fields consisting namely of X-rays and charged particles in terms of particle-types (species), spectral response (energy loss) and direction in wide field-of-view (essentially 2????) with a single compact tracking detector. Tests and calibration measurements were performed with the same device at electron, proton and ion fields at various energies and incident directions. Event-by-event detection, together with pattern recognition analysis of the single particle tracks, are exploited to analyze events according to three degrees of freedom—the particle type (X-rays, light and heavy charged particles), energy range (low or high energy—depending on their range being smaller or larger than the pixel size of the detector semiconductor sensor) and direction (incident angle to the sensor plane). Characteristic values are determined for the cluster analysis morphology parameters, the particles stopping power or Linear Energy Transfer and derived correlated quantities. Ratios and correlations between selected parameters are analyzed including 2D-scatter plots. A physics-based wide-range classification is proposed for a total of 8 broad event groups—in terms of light charged particles (electrons, muons) of both low and high energy incident perpendicular (type 1, including X-rays) or high energy non-perpendicular (type 5), protons of low energy omnidirectional (type 2) and high energy non-perpendicular (type 6), alpha particles and light ions of low energy omnidirectional (type 3) and high energy non-perpendicular (type 7), and heavy ions of low energy omnidirectional (type 4) and high energy non-perpendicular (type 8)

Extremely rapid isotropic irradiation of nanoparticles with ions generated in situ by a nuclear reaction

Mass production of nanoparticles containing well-controlled structural defects is a challenge. Here the authors demonstrate the feasibility of homogeneous ion irradiation generated in a nuclear reactor, for the preparation of fluorescent nanodiamonds and silicon carbide nanoparticles

Wide-range tracking and LET-spectra of energetic light and heavy charged particles

We developed a highly-selective technique to measure the energy loss and linear-energy-transfer (LET) spectra of energetic charged particles in high-resolution and over a large collection of particle-event types. Precise and wide-range spectral and tracking measurements were performed with a single semiconductor pixel detector. The quantum-counting sensitivity, high-granularity and per-pixel spectrometric response of the Timepix ASIC chip enable the detailed spectral-tracking registration of single charged particles across the detector semiconductor sensor. Both the deposited energy along the particle trajectory (energy loss) and the path length of the particle track across the semiconductor sensor are precisely measured for each particle. This allows for the determination of the particle LET in silicon in high accuracy and over a wide-range of energies, particle types and directions. The tracking and energy loss response together with the resolving power at the particle-event level make it possible to selectively provide LET distributions of the light and heavy charged particle components in mixed-radiation and omnidirectional fields. This technique applies to energetic (E > 10 MeV/u) charged particles generating tracks greater than the pixel size and incident at nonperpendicular direction (>20◦) to the sensor plane. The technique applies also to electrons of energy above few MeV as well as highly energetic and minimum-ionizing-particles (MIPs). We make use of existing and in part newly collected data at well-defined radiation fields with proton and light ion beam accelerators. Flexible measurements, ease of deployment and online response are possible by the use of compact readout electronics such as the miniaturized radiation camera MiniPix (size < 8 cm, weight < 50 g) operable by any PC. Results are given for protons and light ions (He, C) of selected energies above 10 MeV/u and directions (2π FoV). We include also electrons (20 MeV). Selective and detailed LET spectra are produced over a wide range (10−1 to 102 keV/μm) in silicon

Mitochondrially targeted vitamin E succinate efficiently kills breast tumour-initiating cells in a complex II-dependent manner

Background: Accumulating evidence suggests that breast cancer involves tumour-initiating cells (TICs), which play a role in initiation, metastasis, therapeutic resistance and relapse of the disease. Emerging drugs that target TICs are becoming a focus of contemporary research. Mitocans, a group of compounds that induce apoptosis of cancer cells by destabilising their mitochondria, are showing their potential in killing TICs. In this project, we investigated mitochondrially targeted vitamin E succinate (MitoVES), a recently developed mitocan, for its in vitro and in vivo efficacy against TICs.Methods: The mammosphere model of breast TICs was established by culturing murine NeuTL and human MCF7 cells as spheres. This model was verified by stem cell marker expression, tumour initiation capacity and chemotherapeutic resistance. Cell susceptibility to MitoVES was assessed and the cell death pathway investigated. In vivo efficacy was studied by grafting NeuTL TICs to form syngeneic tumours.Results: Mammospheres derived from NeuTL and MCF7 breast cancer cells were enriched in the level of stemness, and the sphere cells featured altered mitochondrial function. Sphere cultures were resistant to several established anti-cancer agents while they were susceptible to MitoVES. Killing of mammospheres was suppressed when the mitochondrial complex II, the molecular target of MitoVES, was knocked down. Importantly, MitoVES inhibited progression of syngeneic HER2(high) tumours derived from breast TICs by inducing apoptosis in tumour cells.Conclusions: These results demonstrate that using mammospheres, a plausible model for studying TICs, drugs that target mitochondria efficiently kill breast tumour-initiating cells