16 research outputs found
Anisometric Polyelectrolyte/Mixed Surfactant Nanoassemblies Formed by the Association of Poly(diallyldimethylammonium chloride) with Sodium Dodecyl Sulfate and Dodecyl Maltoside
The soluble complexes of oppositely
charged macromolecules and
amphiphiles, formed in the one-phase concentration range, are usually
described on the basis of the beads on a string model assuming spherelike
bound surfactant micelles. However, around and above the charge neutralization
ionic surfactant to polyion ratio, a variety of ordered structures
of the precipitates and large polyion/surfactant aggregates have been
reported for the different systems which are difficult to connect
to globular-like surfactant self-assembly units. In this article we
have demonstrated through SAXS measurements that the structure of
precipitates and those of the soluble polyion/mixed surfactant complexes
of polyÂ(diallyldimethylammonium chloride) (PDADMAC), sodium dodecyl
sulfate (SDS), and dodecyl-maltoside (DDM) are strongly correlated.
Specifically, SDS binds to the PDADMAC molecules in the form of small
cylindrical surfactant micelles even at very low SDS-to-PDADMAC ratios.
In this way, these anisometric surfactant self-assemblies formed in
excess polyelectrolyte mimic the basic building units of the hexagonal
structure of the PDADMAC/SDS precipitate and/or suspensions formed
at charge equivalence or at higher SDS-to-PDADMAC ratios. The presence
of DDM reduces the cmc and cac for the system but does not alter significantly
the structure of the complexes in either the one-phase or two-phase
region. The only exception is for samples at SDS-to-PDADMAC ratios
close to charge neutralization and a high concentration of DDM where
the precipitate forms a multiphasic or distorted hexagonal structure
Transformation from Globular to Cylindrical Mixed Micelles through Molecular Exchange that Induces Micelle Fusion
Transformations between different
micellar morphologies in solution
induced by changes in composition, salt, or temperature are well-known
phenomena; however, the understanding of the associated kinetic pathways
is still limited. Especially for mixed surfactant systems, the micelles
can take a very wide range of structures, depending on the surfactant
packing parameter and other thermodynamic conditions. Synchrotron-based
small-angle X-ray scattering (SAXS) in combination with fast mixing
using a stopped-flow apparatus can give direct access to the structural
kinetics on a millisecond time scale. Here, this approach is used
to study the formation of cylindrical micelles after mixing two solutions
with globular micelles of the nonionic surfactant dodecyl maltoside
(DDM) and the anionic surfactant sodium dodecyl sulfate (SDS), respectively.
Two separate processes were identified: (i) a transition in micellar
shell structure, interpreted as exchange of surfactant molecules resulting
in mixed globular micelles, and subsequently, (ii) fusion into larger,
cylindrical structures
Self-Healing Mussel-Inspired Multi-pH-Responsive Hydrogels
Self-healing hydrogels can be made using either reversible
covalent
cross-links or coordination chemistry bonds. Here we present a multi-pH-responsive
system inspired by the chemistry of blue mussel adhesive proteins.
By attaching DOPA to an amine-functionalized polymer, a multiresponsive
system is formed upon reaction with iron. The degree of polymer cross-linking
is pH controlled through the pH-dependent DOPA/iron coordination chemistry.
This leads to the formation of rapidly self-healing high-strength
hydrogels when pH is raised from acidic toward basic values. Close
to the p<i>K</i><sub>a</sub> value, or more precisely the
pI value, of the polymer, the gel collapses due to reduced repulsion
between polymer chains. Thereby a bistable gel-system is obtained.
The present polymer system more closely resembles mussel adhesive
proteins than those previously reported and thus also serves as a
model system for mussel adhesive chemistry
Release of Solubilizate from Micelle upon Core Freezing
By combining NMR (yielding <sup>1</sup>H chemical shift, spin relaxation, and self-diffusion data)
and small-angle X-ray scattering experiments, we investigate the complex
temperature dependence of the molecular and aggregate states in aqueous
solutions of the surfactant [CH<sub>3</sub>(CH<sub>2</sub>)<sub>17</sub>(OCH<sub>2</sub>CH<sub>2</sub>)<sub>20</sub>OH], abbreviated as C18E20,
and hexamethyldisiloxane, HMDSO. The latter molecule serves as a model
for hydrophobic solubilizates. Previously, the pure micellar solution
was demonstrated to exhibit core freezing at approximately 7â8
°C. At room temperature, we find that HMDSO solubilizes at a
volume fraction of approximately 10% in the core of the C18E20 micelles,
which consists of molten and thereby highly mobile alkyl chains. Upon
lowering the temperature, core freezing is found, just like in pure
micelles, but at a temperature shifted significantly to 3 °C.
The frozen cores contain immobile alkyl chains and exhibit a higher
density but are essentially devoid (volume fraction below 1%) of the
solubilizate. The latter molecules are released, first gradually and
then rather steeply, from the core in the temperature range that is
roughly delimited by the two core freezing temperatures, one for pure
micelles and one for micelles with solubilizates. The release behavior
of systems with different initial HMDSO loading follows the same master
curve. This feature is rationalized in terms of loading capacity being
strongly temperature dependent: upon lowering the temperature, release
commences once the loading capacity descends below the actual solubilizate
content. The sharp release curves and the actual release mechanism
with its molecular features shown in rich detail have some bearing
on a diverse class of possible applications
Core Freezing and Size Segregation in Surfactant CoreâShell Micelles
Nonionic
surfactants containing polyÂ(ethylene oxide) are chemically simple
and biocompatible and form coreâshell micelles at a wide range
of conditions. For those reasons, they and their aggregates have been
widely investigated. Recently, irregularities that were observed in
the low-temperature behavior of surfactants of the kind [CH<sub>3</sub>(CH<sub>2</sub>)<sub><i>n</i></sub>OÂ(CH<sub>2</sub>CH<sub>2</sub>O)<sub><i>m</i></sub>H], (abbreviated C<i>n</i>E<i>m</i>) were assigned to a freezingâmelting phase
transition in the micellar core. In this work we expand the focus
from the case of single component systems to binary surfactant systems
at temperatures between 1 and 15 °C. By applying small-angle
X-ray scattering (SAXS), differential scanning calorimetry (DSC),
nuclear magnetic resonance (NMR), and density measurements in pure
C18E20 and C18E100 solutions and their mixtures, we show that core
freezing/melting is also present in mixtures. Additionally, comparing
SAXS data obtained from the mixture with those from the single components,
it was possible to demonstrate that the phase transition leads to
a reversible segregation of the surfactants from mixed micelles to
distinct kinds of micelles of the two components
Structures of PEPâPEO Block Copolymer Micelles: Effects of Changing Solvent and PEO Length and Comparison to a Thermodynamic Model
Structures of polyÂ(ethylene propylene)âpolyÂ(ethylene
oxide) (PEPâPEO) block copolymer micelles were determined from
small-angle X-ray scattering and static light scattering and compared
to predictions from a thermodynamic model. Both the corona block length
and the solvent waterâethanol ratio were changed, leading to
a thorough test of this model. With increasing ethanol fraction, the
PEP coreâsolvent interfacial tension decreases, and the solvent
quality for PEO changes. The weight-average block masses were 5.0
kDa for PEP and 2.8â49 kDa for PEO. For the lowest PEO molar
mass and samples in pure water (except for the highest PEO molar mass),
the micelles were cylindrical; for other conditions they were spherical.
The structural parameters can be reasonably well described by the
thermodynamic model by Zhulina et al. [<i>Macromolecules</i> <b>2005</b>, <i>38</i> (12), 5330â5351];
however, they have a stronger dependence on solvent composition and
PEO molar mass than predicted
Direct Observation of the Formation of Surfactant Micelles under Nonisothermal Conditions by Synchrotron SAXS
Self-assembly
of amphiphilic molecules into micelles occurs on very short times
scales of typically some milliseconds, and the structural evolution
is therefore very challenging to observe experimentally. While rate
constants of surfactant micelle kinetics have been accessed by spectroscopic
techniques for decades, so far no experiments providing detailed information
on the structural evolution of surfactant micelles during their formation
process have been reported. In this work we show that by applying
synchrotron small-angle X-ray scattering (SAXS) in combination with
the stopped-flow mixing technique, the entire micelle formation process
from single surfactants to equilibrium micelles can be followed in
situ. Using a sugar-based surfactant system of dodecyl maltoside (DDM)
in dimethylformamide (DMF), micelle formation can be induced simply
by adding water, and this can be followed in situ by SAXS. Mixing
of water and DMF is an exothermic process where the micelle formation
process occurs under nonisothermal conditions with a temperature gradient
relaxing from about 40 to 20 °C. A kinetic nucleation and growth
mechanism model describing micelle formation by insertion/expulsion
of single molecules under nonisothermal conditions was developed and
shown to describe the data very well
High Electrokinetic Energy Conversion Efficiency in Charged Nanoporous Nitrocellulose/Sulfonated Polystyrene Membranes
The
synthesis, characterization, and electrokinetic energy conversion
performance have been investigated experimentally in a charged polymeric
membrane based on a blend of nitrocellulose and sulfonated polystyrene.
The membrane is characterized by a moderate ion exchange capacity
and a relatively porous structure with average pore diameter of 11
nm. With electrokinetic energy conversion, pressure can be converted
directly into electric energy and vice versa. From the electrokinetic
transport properties, a remarkably large intrinsic maximum efficiency
of 46% is found. It is anticipated that the results are an experimental
verification of theoretical models that predict high electrokinetic
energy conversion efficiency in pores with high permselectivity and
hydrodynamic slip flow. Furthermore, the result is a promising step
for obtaining efficient low-cost electrokinetic generators and pumps
for small or microscale applications
How Hollow Are Thermoresponsive Hollow Nanogels?
A main challenge in colloid science
is the development of smart
delivery systems that store and protect actives from degradation and
allow release in response to an external stimulus like temperature.
Hollow nanogel capsules made of temperature-sensitive polymers are
particularly promising materials. The stimuli-sensitive void size,
shell thickness, and permeability determine cargo storage and its
release behavior. Thus, determination and control of these morphological
parameters are of outmost relevance for the design of new, functional
drug delivery vehicles. Here we investigate quantitatively void size
and shell thickness of hollow nanogels at different states of swelling
by means of small-angle neutron scattering (SANS) employing contrast
variation. We demonstrate the structure-sensitivity dilemma: hollow
nanogels with a slightly cross-linked shell reveal distinct temperature
sensitivity but possess nearly no void (14% of the initial core volume)
and are thus hardly âhollowâ. Nanogels with a stiff
shell are indeed hollow (albeit with smaller void as compared to the
core size of the template) but less temperature sensitive
Glycolipid Biosurfactants Activate, Dimerize, and Stabilize <i>Thermomyces lanuginosus</i> Lipase in a pH-Dependent Fashion
We
present a study of the interactions between the lipase from <i>Thermomyces lanuginosus</i> (TlL) and the two microbially produced
biosurfactants (BSs), rhamnolipid (RL) and sophorolipid (SL). Both
RL and SL are glycolipids; however, RL is anionic, while SL is a mixture
of anionic and non-ionic species. We investigate the interactions
of RL and SL with TlL at pH 6 and 8 and observe different effects
at the two pH values. At pH 8, neither RL nor SL had any major effect
on TlL stability or activity. At pH 6, in contrast, both surfactants
increase TlLâs thermal stability and fluorescence and activity
measurements indicate interfacial activation of TlL, resulting in
3- and 6-fold improved activity in SL and RL, respectively. Nevertheless,
isothermal titration calorimetry reveals binding of only a few BS
molecules per lipase. Size-exclusion chromatography and small-angle
X-ray scattering suggest formation of TlL dimers with binding of small
amounts of either RL or SL at the dimeric interface, forming an elongated
complex. We conclude that RL and SL are compatible with TlL and constitute
promising green alternatives to traditional surfactants