337 research outputs found
Peptide synthesis by recombinant Fasciola hepatica cathepsin L1
Synthesis of the tripeptide Z-Phe-Arg-SerNH2 has been accomplished by a recombinant cysteine protease, cathepsin L1 from liver fluke (Fasciola hepatica), using Z-Phe-Arg-OMe as acyl acceptor and SerNH2 as nucleophile in 0.1 M ammonium acetate pH 9.0â12.5% v/v acetonitrile at 37 °C. LCâMS detection indicated tripeptide formation after 10 min, continuing up to 5.5 h. The ester Z-Phe-Arg-OMe was detected throughout the experiment but the hydrolysis product Z-Phe-Arg-OH appeared early and in quite large amounts. We believe that this is the first application of a parasite protease in enzymatic peptide synthesis
Nonconventional amide bond formation catalysis: programming enzyme specificity with substrate mimetics
Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis.
Peptide arrays on cellulose are a powerful tool to investigate peptide interactions with a number of different molecules, for examples antibodies, receptors or enzymes. Such peptide arrays can also be used to study interactions with whole cells. In this review, we focus on the interaction of small antimicrobial peptides with bacteria. Antimicrobial peptides (AMPs) can kill multidrug-resistant (MDR) human pathogenic bacteria and therefore could be next generation antibiotics targeting MDR bacteria. We describe the screen and the result of different optimization strategies of peptides cleaved from the membrane. In addition, screening of antibacterial activity of peptides that are tethered to the surface is discussed. Surface-active peptides can be used to protect surfaces from bacterial infections, for example implants
ChemInform Abstract: METHODISCHE UNTERSUCHUNGEN ZUR MERRIFIELD-SYNTHESE 2. MITT. VERGLEICHENDE STUDIEN UEBER DEN PEPTIDKNUEPFUNGSSCHRITT UNTER VERWENDUNG VERSCHIEDENER AKTIVIERTER ESTER AM BEISPIEL EINES MODELLPEPTIDS
ChemInform Abstract: METHODISCHE UNTERSUCHUNGEN ZUR MERRIFIELD-SYNTHESE 1. MITT. STUDIEN UEBER DEN EINSATZ DER MISCHANHYDRID-, WOODWARD- UND CARBODIIMID-METHODE AN HAND EINES MODELLPEPTIDS
ChemInform Abstract: LEWIS ACIDS, ESPECIALLY ZINC CHLORIDE- A NEW TYPE OF CARBODIMIDE ADDITIVE IN PEPTIDE SYNTHESIS
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