4 research outputs found
Differential Surface Interactions and Surface Templating of Nucleotides (dGMP, dCMP, dAMP, and dTMP) on Oxide Particle Surfaces
The fate of biomolecules in the environment depends in
part on
understanding the surface chemistry occurring at the biological–geochemical
(bio–geo) interface. Little is known about how environmental
DNA (eDNA) or smaller components, like nucleotides and oligonucleotides,
persist in aquatic environments and the role of surface interactions.
This study aims to probe surface interactions and adsorption behavior
of nucleotides on oxide surfaces. We have investigated the interactions
of individual nucleotides (dGMP, dCMP, dAMP, and dTMP) on TiO2 particle surfaces as a function of pH and in the presence
of complementary and noncomplementary base pairs. Using attenuated
total reflectance-Fourier transform infrared spectroscopy, there is
an increased number of adsorbed nucleotides at lower pH with a preferential
interaction of the phosphate group with the oxide surface. Additionally,
differential adsorption behavior is seen where purine nucleotides
are preferentially adsorbed, with higher surface saturation coverage,
over their pyrimidine derivatives. These differences may be a result
of intermolecular interactions between coadsorbed nucleotides. When
the TiO2 surface was exposed to two-component solutions
of nucleotides, there was preferential adsorption of dGMP compared
to dCMP and dTMP, and dAMP compared to dTMP and dCMP. Complementary
nucleotide base pairs showed hydrogen-bond interactions between a
strongly adsorbed purine nucleotide layer and a weaker interacting
hydrogen-bonded pyrimidine second layer. Noncomplementary base pairs
did not form a second layer. These results highlight several important
findings: (i) there is differential adsorption of nucleotides; (ii)
complementary coadsorbed nucleotides show base pairing with a second
layer, and the stability depends on the strength of the hydrogen bonding
interactions and; (iii) the first layer coverage strongly depends
on pH. Overall, the importance of surface interactions in the adsorption
of nucleotides and the templating of specific interactions between
nucleotides are discussed
Surface Analysis
Surface analysis of water soluble proteins as a function of surface chemistr
Enzyme-Polymer Simulation
Simulation of protein-random heteropolymer complex in solution
Sequence simulation of random heteropolymer
The file contains simulated sequences of random heteropolymers and block length histogram analysis