Genetic Map of Bacteriophage [var phi]X174

Bacteriophage [var phi]X174 temperature-sensitive and nonsense mutations in eight cistrons were mapped by using two-, three-, and four-factor genetic crosses. The genetic map is circular with a total length of 24 × 10−4wt recombinants per progeny phage. The cistron order is D-E-F-G-H-A-B-C. High negative interference is seen, consistent with a small closed circular deoxyribonucleic acid molecule as a genome

Observations on the Overwintering Potential of the Striped Cucumber Beetle (Coleoptera: Chrysomelidae) in Southern Minnesota

The striped cucumber beetle, Acalymma vittatum (Fabricius) (Coleoptera: Chrysomelidae), is an important pest of cucurbit crops. However, the overwinter- ing capacity of this pest in temperate regions is poorly understood. In this study, the in-field survival of A. vittatum was examined during three consecutive winters. In addition, the supercooling points of A. vittatum were determined as an index of cold hardiness for adults. During each winter, the survival of adults decreased significantly through time, with no individuals surviving until spring. By comparing the supercooling points and in-field survival of adults to soil temperatures, it appears that winter temperatures in Minnesota are cold enough to induce freezing of the beetles. Moreover, a considerable amount of mortality occurred before minimum monthly soil temperatures dropped below the supercooling point of overwintering individuals, suggesting the occurrence of prefreeze mortality. An improved understanding of the response of A. vittatum to winter temperatures in temperate regions may aid in early season management of this pest

Theoretical And Experimental Studies Of Collision-Induced Electronic Energy Transfer From v=0-3 Of The E(0g+) Ion-Pair State Of Br2: Collisions With He And Ar

Collisions of Br(2), prepared in the E(0(g)(+)) ion-pair (IP) electronic state, with He or Ar result in electronic energy transfer to the D, D(\u27), and beta IP states. These events have been examined in experimental and theoretical investigations. Experimentally, analysis of the wavelength resolved emission spectra reveals the distribution of population in the vibrational levels of the final electronic states and the relative efficiencies of He and Ar collisions in promoting a specific electronic energy transfer channel. Theoretically, semiempirical rare gas-Br(2) potential energy surfaces and diabatic couplings are used in quantum scattering calculations of the state-to-state rate constants for electronic energy transfer and distributions of population in the final electronic state vibrational levels. Agreement between theory and experiment is excellent. Comparison of the results with those obtained for similar processes in the IP excited I(2) molecule points to the general importance of Franck-Condon effects in determining vibrational populations, although this effect is more important for He collisions than for Ar collisions

Electrically-detected magnetic resonance in ion-implanted Si:P nanostructures

We present the results of electrically-detected magnetic resonance (EDMR) experiments on silicon with ion-implanted phosphorus nanostructures, performed at 5 K. The devices consist of high-dose implanted metallic leads with a square gap, into which Phosphorus is implanted at a non-metallic dose corresponding to 10^17 cm^-3. By restricting this secondary implant to a 100 nm x 100 nm region, the EDMR signal from less than 100 donors is detected. This technique provides a pathway to the study of single donor spins in semiconductors, which is relevant to a number of proposals for quantum information processing.Comment: 9 pages, 3 figure

Tuning the Kondo effect with a mechanically controllable break junction

We study electron transport through C60 molecules in the Kondo regime using a mechanically controllable break junction. By varying the electrode spacing, we are able to change both the width and height of the Kondo resonance, indicating modification of the Kondo temperature and the relative strength of coupling to the two electrodes. The linear conductance as a function of T/T_K agrees with the scaling function expected for the spin-1/2 Kondo problem. We are also able to tune finite-bias Kondo features which appear at the energy of the first C60 intracage vibrational mode.Comment: 4 pages with 4 figure

Atomic environments in iron meteorites using EXAFS

Extended x ray absorption fine structure (EXAFS) is observed as a modulation on the high energy side of an x ray absorption edge. It occurs when the photo-ejected electron wave is scattered by neighboring atoms in a solid, and interference occurs between the outgoing and scattered waves. The result is that the absorption spectrum carries a signature that is characteristic of the identity and disposition of scattering atoms around the absorbing atom. Therefore, it may be shown that the Fourier transform of the normalized EXAFS can provide detailed information about the immediate environment of specific atoms in a solid and is ideally suited to the study of cosmic dusts. A study of cosmic dust was initiated using EXAFS and other techniques. The simplest type of cosmic material, namely iron meteorites, was investigated

Clinical decision making in paramedic practice. A qualitative study exploring the challenges of responding to mental health needs.

The paramedic service responds to emergency calls for a variety of reasons with many relating to mental health concerns. This qualitative study aims to explore the views and experiences of student paramedics in relation to clinical decision making for mental health calls. Focus groups were utilised to investigate the participants’ perspectives. Thematic analysis was used to organise data and identify key issues. Findings suggested some disparity between what paramedic students were prepared for and the reality of public need. Clinical decision-making in relation to those with mental health problems was significantly influenced by the current provision of mental health services and the lack of mental health specific education for student paramedics. Current changes to the paramedic programme make this an opportune time for a review of curriculum content