Predation by \u3ci\u3eAchaearanea Tepidariorum\u3c/i\u3e (Araneae: Theridiidae) on \u3ci\u3eAnoplophora Glabripennis\u3c/i\u3e (Coleoptera: Cerambycidae)

Anoplophora glabripennis is a large wood-boring cerambycid beetle that has recently invaded North America and Europe from Asia. We discovered the common house spider, Achaearanea tepidariorum, in large cages housing A. glabripennis on trees and confirmed the ability of A. tepidariorum to prey upon adult A. glabripennis by placing the two species together within smaller containers where they could be more easily observed. Adult A. glabripennis, up to 600% of the spiders’ body length, exceed the maximum relative size of prey previously reported for A. tepidariorum or for solitary webbuilding spiders in general

Insect Floral Visitors of Red Maple and Tree-of-Heaven at Potential Risk of Neonicotinoid Residue Exposure from Spotted Lanternfly Control

To manage spotted lanternfly (Lycorma delicatula (White)), an invasive planthopper recently introduced to the United States, governmental agencies, homeowners, and landscape professionals may treat its host plants with systemic neonicotinoid insecticides. Neonicotinoids can be harmful to non-target insect floral visitors collecting pollen or nectar. The insect floral visitors of red maple (Acer rubrum L.) or tree-of-heaven (Ailanthus altissima (Mill.), which are two of the preferred hosts of L. delicatula in Pennsylvania, are often targeted with neonicotinoid applications for control of this pest. To identify the floral visitors of these tree species, we conducted observations and collections of insects foraging on the flowers of these trees in Pennsylvania during the annual bloom period. In southeastern Pennsylvania, the margined leatherwing beetle (Chauliognathus marginatus (F.)). was the most dominant visitor of tree-of-heaven flowers, followed in abundance by bees, particularly Lasioglossum and Bombus spp., and flies. In central and southeastern Pennsylvania, bees were the most dominant group to visit red maple flowers, particularly Apis mellifera (L.), Andrena spp. and Osmia spp., followed by flies. These results contribute to our knowledge of the pool of bioindicator insects that may be at risk from exposure to neonicotinoid residues in flowers of trees treated for L. delicatula management

Research poster: Eco-hydrological pathways inferred from stable isotopes in a Pinus ponderosa and Pinus monophylla woodland of the Sheep Range, southern Great Basin, USA

Research poste

Induction of Systemic Acquired Resistance in Cotton Foliage Does Not Adversely Affect the Performance of an Entomopathogen

Baculoviral efficacy against lepidopteran larvae is substantially impacted by the host plant. Here, we characterized how baculoviral pathogenicity to cotton-fed Heliothis virescens larvae is affected by induction of systemic acquired resistance (SAR). Numerous studies have shown that SAR induced by the plant elicitor benzo-(1,2,3)-thiadiazole-7- carbothioic acid S-methyl ester (BTH) can protect against plant pathogens, but reports on the impacts of SAR on chewing herbivores or on natural enemies of herbivores are few. We found that BTH application significantly increased foliar peroxidase activity, condensed tannin levels, and total phenolic levels but did not alter dihydroxyphenolic levels. Consumption of BTH-treated foliage did not influence H. virescens pupal weight or larval mortality by the microbial control agent Autographa californica multiple nucleopolyhedrovirus any more than did consumption of untreated foliage. Thus, activation of SAR, although it did not protect the plant against a chewing herbivore, also did not reduce the effect of a natural enemy on a herbivore, indicating that SAR and microbial control agents may be compatible components of integrated pest management

Plant genotype and induced defenses affect the productivity of an insect-killing obligate viral pathogen

© 2017 Elsevier Inc. Plant-mediated variations in the outcomes of host-pathogen interactions can strongly affect epizootics and the population dynamics of numerous species, including devastating agricultural pests such as the fall armyworm. Most studies of plant-mediated effects on insect pathogens focus on host mortality, but few have measured pathogen yield, which can affect whether or not an epizootic outbreak occurs. Insects challenged with baculoviruses on different plant species and parts can vary in levels of mortality and yield of infectious stages (occlusion bodies; OBs). We previously demonstrated that soybean genotypes and induced anti-herbivore defenses influence baculovirus infectivity. Here, we used a soybean genotype that strongly reduced baculovirus infectivity when virus was ingested on induced plants (Braxton) and another that did not reduce infectivity (Gasoy), to determine how soybean genotype and induced defenses influence OB yield and speed of kill. These are key fitness measures because baculoviruses are obligate-killing pathogens. We challenged fall armyworm, Spodoptera frugiperda, with the baculovirus S. frugiperda multi-nucleocapsid nucleopolyhedrovirus (SfMNPV) during short or long-term exposure to plant treatments (i.e., induced or non-induced genotypes). Caterpillars were either fed plant treatments only during virus ingestion (short-term exposure to foliage) or from the point of virus ingestion until death (long-term exposure). We found trade-offs of increasing OB yield with slower speed of kill and decreasing virus dose. OB yield increased more with longer time to death and decreased more with increasing virus dose after short-term feeding on Braxton compared with Gasoy. OB yield increased significantly more with time to death in larvae that fed until death on non-induced foliage than induced foliage. Moreover, fewer OBs per unit of host tissue were produced when larvae were fed induced foliage than non-induced foliage. These findings highlight the potential importance of plant effects, even at the individual plant level, on entomopathogen fitness, which may impact epizootic transmission events and host population dynamics

Plant-mediated Alteration of the Peritrophic Matrix and Baculovirus Infection in Lepidopteran Larvae

The peritrophic matrix (PM) lines the midgut of most insects, providing protection to the midgut epithelial cells while permitting passage of nutrients and water. Herein, we provide evidence that plant-mediated alteration of the PM contributes to the well-documented inhibition of fatal infection by Autographa californica multiple nucleopolyhedrovirus (AcMNPV) of Heliothis virescens F. larvae fed cotton foliage. We examined the impact of the PM on pathogenesis using a viral construct expressing a reporter gene (AcMNPV-hsp70/ lacZ) orally inoculated into larvae with either intact PMs or PMs disrupted by Trichoplusia ni granulovirus occlusion bodies containing enhancin, known to degrade insect intestinal mucin. Larvae possessing disrupted PMs displayed infection foci (lacZ signaling) earlier than those with intact PMs. We then examined PMs from larvae fed artificial diet or plant foliage using electron microscopy; foliage-fed larvae had significantly thicker PMs than diet-fed larvae. Moreover, mean PM width was inversely related to both the proportion of larvae with lacZ signaling at 18 h post-inoculation and the final percentage mortality from virus. Thus, feeding on foliage altered PM structure, and these foliage-mediated changes reduced baculoviral efficacy. These data indicate that the PM is an important factor determining the success of an ingested pathogen in foliage-fed lepidopteran larvae

Functional genomics and microbiome profiling of the Asian longhorned beetle (\u3ci\u3eAnoplophora glabripennis\u3c/i\u3e) reveal insights into the digestive physiology and nutritional ecology of wood feeding beetles

Background: Wood-feeding beetles harbor an ecologically rich and taxonomically diverse assemblage of gut microbes that appear to promote survival in woody tissue, which is devoid of nitrogen and essential nutrients. Nevertheless, the contributions of these apparent symbionts to digestive physiology and nutritional ecology remain uncharacterized in most beetle lineages. Results: Through parallel transcriptome profiling of beetle- and microbial- derived mRNAs, we demonstrate that the midgut microbiome of the Asian longhorned beetle (Anoplophora glabripennis), a member of the beetle family Cerambycidae, is enriched in biosynthetic pathways for the synthesis of essential amino acids, vitamins, and sterols. Consequently, the midgut microbiome of A. glabripennis can provide essential nutrients that the beetle cannot obtain from its woody diet or synthesize itself. The beetle gut microbiota also produce their own suite of transcripts that can enhance lignin degradation, degrade hemicellulose, and ferment xylose and wood sugars. An abundance of cellulases from several glycoside hydrolase families are expressed endogenously by A. glabripennis, as well as transcripts that allow the beetle to convert microbe-synthesized essential amino acids into non-essential amino acids. A. glabripennis and its gut microbes likely collaborate to digest carbohydrates and convert released sugars and amino acid intermediates into essential nutrients otherwise lacking from their woody host plants. Conclusions: The nutritional provisioning capabilities of the A. glabripennis gut microbiome may contribute to the beetles’ unusually broad host range. The presence of some of the same microbes in the guts of other Cerambycidae and other wood-feeding beetles suggests that partnerships with microbes may be a facilitator of evolutionary radiations in beetles, as in certain other groups of insects, allowing access to novel food sources through enhanced nutritional provisioning

Sensory Aspects of Trail-Following Behaviors in the Asian Longhorned Beetle, Anoplophora glabripennis

Abstract Anoplophora glabripennis has a complex suite of mate-finding behaviors, the functions of which are not entirely understood. These behaviors are elicited by a number of factors, including visual and chemical cues. Chemical cues include a maleproduced volatile semiochemical acting as a long-range sex pheromone, a femaleproduced cuticular hydrocarbon blend serving as a sex-identification contact pheromone, and a recently identified female-produced trail sex pheromone that is followed by mate-seeking males. However, the sensory appendages and sensilla on these appendages used to detect the trail sex pheromone are unknown. We evaluated the ability of virgin male A. glabripennis to follow a sex pheromone trail after removal of the terminal four antennal segments and/or the maxillary and labial palps using a twochoice behavioral bioassay. We also tested the ability of males to follow the trail sex pheromone using volatile pheromone cues only, without physical contact with the pheromone. Results indicate that the palps are primarily responsible for sensing the pheromone, with males lacking palps unable to respond behaviorally to the trail sex pheromone. Under the conditions of this study, males could not follow the sex pheromone trail without direct contact, suggesting that olfaction may not be involved in detection of this pheromone. However, we did not determine to what degree the trail pheromone chemicals can volatilize under our experimental conditions. This work is important in elucidating the behaviors and sensory structures involved in mate-finding J Insect Behav (2016) by this species on host trees, and these studies may help determine whether the trail sex pheromone has applications for monitoring and management

STS Research Agenda Task Force: Survey Results

As part of our charge to provide STS members with recommended areas for exploration in science and technology library research, we sent out a survey in the Fall of 2014. The 258 responses helped create a snapshot of what areas are currently being researched and what could be important areas of future research.Poster presented at the ACRL 2015 Conference in Portland, OR.UT Librarie