133 research outputs found
Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Plant Breeding/Biotechnology; Biological Techniques; Nucleic Acid Chemistr
Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Plant Breeding/Biotechnology; Biological Techniques; Nucleic Acid Chemistr
Regularization of statistical inverse problems and the Bakushinskii veto
In the deterministic context Bakushinskii's theorem excludes the existence of
purely data driven convergent regularization for ill-posed problems. We will
prove in the present work that in the statistical setting we can either
construct a counter example or develop an equivalent formulation depending on
the considered class of probability distributions. Hence, Bakushinskii's
theorem does not generalize to the statistical context, although this has often
been assumed in the past. To arrive at this conclusion, we will deduce from the
classic theory new concepts for a general study of statistical inverse problems
and perform a systematic clarification of the key ideas of statistical
regularization.Comment: 20 page
Measurement of neutron capture on Ca at thermal and thermonuclear energies
At the Karlsruhe pulsed 3.75\,MV Van de Graaff accelerator the thermonuclear
Ca(n,)Ca(8.72\,min) cross section was measured by the
fast cyclic activation technique via the 3084.5\,keV -ray line of the
Ca-decay. Samples of CaCO enriched in Ca by 77.87\,\% were
irradiated between two gold foils which served as capture standards. The
capture cross-section was measured at the neutron energies 25, 151, 176, and
218\,keV, respectively. Additionally, the thermal capture cross-section was
measured at the reactor BR1 in Mol, Belgium, via the prompt and decay
-ray lines using the same target material. The
Ca(n,)Ca cross-section in the thermonuclear and thermal
energy range has been calculated using the direct-capture model combined with
folding potentials. The potential strengths are adjusted to the scattering
length and the binding energies of the final states in Ca. The small
coherent elastic cross section of Ca+n is explained through the nuclear
Ramsauer effect. Spectroscopic factors of Ca have been extracted from
the thermal capture cross-section with better accuracy than from a recent (d,p)
experiment. Within the uncertainties both results are in agreement. The
non-resonant thermal and thermonuclear experimental data for this reaction can
be reproduced using the direct-capture model. A possible interference with a
resonant contribution is discussed. The neutron spectroscopic factors of
Ca determined from shell-model calculations are compared with the values
extracted from the experimental cross sections for Ca(d,p)Ca and
Ca(n,)Ca.Comment: 15 pages (uses Revtex), 7 postscript figures (uses psfig), accepted
for publication in PRC, uuencoded tex-files and postscript-files also
available at ftp://is1.kph.tuwien.ac.at/pub/ohu/Ca.u
Analysis of small RNAs in the Zymoseptoria tritici – wheat interaction
Cross-kingdom small RNA (sRNA) silencing has recently emerged as a mechanism facilitating fungal colonisation and disease development in plants. Here we characterise RNAi pathways in Zymoseptoria tritici, a major fungal pathogen of wheat, and assess their contribution to pathogenesis. Computational analysis of fungal sRNA and wheat mRNA sequencing datasets was used to define the global sRNA populations in Z. tritici and predict putative mRNA targets in wheat. In total, 389 in planta-induced sRNA loci were identified in Z. tritici. sRNAs generated from some of these loci were predicted to target wheat mRNAs, including some that have previously been implicated in defence against pathogens. However, biochemical approaches were unable to successfully validate targeting of selected wheat mRNAs by fungal sRNAs. Z. tritici gene deletion strains deficient for key RNAi components were generated and virulence bioassays suggested that these are dispensable for full infection of wheat. Nonetheless, our results do point to the existence of non-canonical Dicer-independent pathway(s) for sRNA biogenesis in Z. tritici. dsRNA applied in vitro or generated from an RNA virus vector in planta was ineffective at triggering gene silencing or reducing growth of Z. tritici. We conclude that neither in vitro nor in planta RNAi approaches are likely to be useful for gene function analyses or as a viable control measure
for this pathogen
Analysis of small RNA silencing in Zymoseptoria tritici – wheat interactions
Cross-kingdom small RNA (sRNA) silencing has recently emerged as a mechanism facilitating fungal colonization and disease development. Here we characterized RNAi pathways in Zymoseptoria tritici, a major fungal pathogen of wheat, and assessed their contribution to pathogenesis. Computational analysis of fungal sRNA and host mRNA sequencing datasets was used to define the global sRNA populations in Z. tritici and predict their mRNA targets in wheat. 389 in planta-induced sRNA loci were identified. sRNAs generated from some of these loci were predicted to target wheat mRNAs including those potentially involved in pathogen defense. However, molecular approaches failed to validate targeting of selected wheat mRNAs by fungal sRNAs. Mutant strains of Z. tritici carrying deletions of genes encoding key components of RNAi such as Dicer-like (DCL) and Argounate (AGO) proteins were generated, and virulence bioassays suggested that these are dispensable for full infection of wheat. Nonetheless, our results did suggest the existence of non-canonical DCL-independent pathway(s) for sRNA biogenesis in Z. tritici. dsRNA targeting essential fungal genes applied in vitro or generated from an RNA virus vector in planta in a procedure known as HIGS (Host-Induced Gene Silencing) was ineffective in preventing Z. tritici growth or disease. We also demonstrated that Z. tritici is incapable of dsRNA uptake. Collectively, our data suggest that RNAi approaches for gene function analyses in this fungal species and potentially also as a control measure may not be as effective as has been demonstrated for some other plant pathogenic fungi
sRNA profiling combined with gene function analysis reveals a lack of evidence for cross-kingdom RNAi in the wheat – Zymoseptoria tritici pathosystem
Cross-kingdom small RNA (sRNA) silencing has recently emerged as a mechanism facilitating fungal colonization and disease development. Here we characterized RNAi pathways in Zymoseptoria tritici, a major fungal pathogen of wheat, and assessed their contribution to pathogenesis. Computational analysis of fungal sRNA and host mRNA sequencing datasets was used to define the global sRNA populations in Z. tritici
and predict their mRNA targets in wheat. 389 in planta-induced sRNA loci were identified. sRNAs generated from some of these loci were predicted to target wheat mRNAs including those potentially involved in pathogen defense. However, molecular approaches failed to validate targeting of selected wheat mRNAs by fungal sRNAs. Mutant strains of Z. tritici carrying deletions of genes encoding key components of RNAi such as Dicer-like (DCL) and Argonaute (AGO) proteins were generated, and virulence bioassays suggested that these are dispensable for full infection of wheat. Nonetheless, our results did suggest the existence of non-canonical DCL-independent pathway(s) for sRNA biogenesis in Z. tritici. dsRNA targeting essential fungal genes applied in vitro or generated from an RNA virus vector in planta in a procedure known as HIGS (Host-Induced Gene Silencing) was ineffective in preventing Z. tritici growth or disease. We also demonstrated that Z. tritici is incapable of dsRNA uptake. Collectively, our data suggest that RNAi approaches for gene function analyses in this fungal species and potentially also as a control measure may not be as effective as has been demonstrated for some other plant pathogenic fungi
Reaction rates for Neutron Capture Reactions to C-, N- and O-isotopes to the neutron rich side of stability
The reaction rates of neutron capture reactions on light nuclei are important
for reliably simulating nucleosynthesis in a variety of stellar scenarios.
Neutron capture reaction rates on neutron-rich C-, N-, and O-isotopes are
calculated in the framework of a hybrid compound and direct capture model. The
results are tabulated and compared with the results of previous calculations as
well as with experimental results.Comment: 33 pages (uses revtex) and 9 postscript figures, accepted for
publication in Phys. Rev.
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