Characterization of Oxidative Lipidomics and Autophagy Induction in Chlamydomonas reinhardtii Under Abiotic Stress

Autophagy constitutes an essential process triggered upon by oxidative stress that enables cells to recycle damaged biomolecules and organelles, which is eventually traced by immunodetection with anti-ATG8. In parallel with autophagy induction, carbon metabolism in Chlamydomonas reinhardtii under abiotic stress is diverged toward lipid biosynthesis and lipid droplet accumulation, which can be analyzed by a simple thin-layer chromatography and in vivo staining with the fluorescent probe BODIPY 493/503. We show the responses in Chlamydomonas cells exposed to mercury or cadmium (0–50 μM doses), as examples of oxidative stress-mediated changes in autophagy and lipid metabolism, monitored with the procedures described in this repor

Prótesis total de rodilla sobre osteotomía tibial

El objetivo del estudio es conocer las dificultades técnicas de la colocación de una prótesis total sobre una rodilla tratada previamente con una osteotomía tibial y su influencia en el resultado final. Para ello se ha realizado un estudio comparativo entre estos casos (16 rodillas) y un grupo control (116 rodillas). La evaluación clínica, funcional y radiológica de las rodillas se realizó según el sistema de puntuación de la sociedad americana de rodilla. En los casos con osteotomía tibial previa la mejoría clínica fue menor, con una mayor proporción de malos resultados y con una colocación más defectuosa del componente tibial.The aim of this study is to know weather a previous tibial osteotomy has influence in placing a total knee arthroplasty as well as its influence in the outcome of these cases. Therefore, we have done a comparative study between knees with previous osteotomy (16 knees) and a control group (116 knees). Functional, clinical and radiological evaluation was done according to American Knee Society score system. The improvement in cases with a previous tibial osteotomy was less than in the control group. In addititon, this cases showed an increase of bad results and, furthermore, a more frequent deficient placing of the tibial component

PREPARATION AND MANAGEMENT OF THE MSc PROGRAME IN ENVIRONMENTAL SECURITY

Authors proposed an MSc Program related to Environmental Security and Management. This program endeavors to prepare students for the regional and global postgraduate job market

TEACHING STRATEGIES IN THE MSc PROGRAME IN CLIMATE CHANGE AND RESTORATION ON DEGRADED LAND

UPM is a leader on landslide assessment and environmental restoration, as well as in waste management. The study of climate change and degraded land requires innovative techniques in teaching that will be analyzed and discussed in the following paper

Aplicación de namaticidas para controlar el nemátodo quiste de la papa en el altiplano de Nariño.

Con el objeto de controlar el nemátodo quiste de la papa, se llevaron a cabo ocho experimentos con nematicidas no fumigantes. Se utilizó un diseño de bloques al azar con 4 repeticiones. Los nematicidas se aplicaron en banda en el surco al momento de la siembra. Los resultados indican que no hay un claro efecto de los nematicidas en la multiplicación del nemátodo quiste de la papa con excepción de Nemacur (La Laguna) y de algunos productos en Catambuco. Se encontró que las variedades que se siembran en Nariño pueden soportar una alta población del nemátodo (50-70 lv/gr de suelo) sin sufrir daño. Se encontraron dosis óptimas para incrementos en un 30 por ciento en rendimiento, son éstos : aconem 10G 60 kg/ha, furadan 3G y temik 10G, 50 kg/ha. Dasanit 5G actuó bién en dosis de 100 kg/ha pero fué fitotóxico. En relación a vydate 240L con dosis de 251/ha dió buenos resultados pero inconsistentes. Con dosis divididas, 2/3 de la dosis a la siembra y 1/3 antes del primer aporque se han conseguido buenos resultadosPapa-Solanum tuberosu

Nuevos datos geocronológicos 40Ar-39Ar en dos puntos singulares del macizo de Anaga (Tenerife): Punta Poyata e Igueste de San Andrés. Consecuencias de interés

En este trabajo se aportan nuevos datos geocronológicos Ar40-Ar39 en rocas ancaramíticas submarinas localizadas en dos puntos singulares del macizo de Anaga (NE de Tenerife): las ancaramitas submarinas hidrotermalizadas de Punta Poyata (NNW de Anaga) y las lavas submarinas ancaramíticas del Barranco de Igueste (SE de Anaga). Las muestras estudiadas pertenecen a afloramientos pertenecientes a los niveles estratigráficos más inferiores de la secuencia volcánica que conforma el edificio de Anaga. En Punta Poyata se tomaron dos especímenes que dieron edades de 12,2 ± 1,9 Ma and 13,0 ± 1,0 Ma. En el barranco de Igueste los resultados geocronológicos de las muestras tomadas dieron edades de 12,7 ± 1,6 Ma and 9,7 ± 1,0 Ma. Estos datos sugieren que el macizo de Anaga comenzó su desarrollo subaéreo antes de lo que anteriormente se creía, ajustándose dicho período inicial al Mioceno Medio en lugar de al Mioceno tardío como previamente se asumía.This work provides new Ar40-Ar39 geochronological data in submarine ankaramite rocks located in two distant points of Anaga (NE Tenerife): the submarine hydrothermally altered ankaramites of Punta Poyata area (NNW Anaga) and the ankaramitic pillow lavas at Barranco de Igueste (SE Anaga). The studied samples were collected from outcrops of the lowest stratigraphic levels throughout the volcanics that build up the Anaga massif. At Punta Poyata, two collected samples yielded an age of 12,2 ± 1,9 Ma and 13,0 ± 1,0 Ma. At Barranco de Igueste, sample results are of 12,7 ± 1,6 Ma and 9,7 ± 1,0 Ma. These data support the assumption that the Anaga massif began its subaerial growth earlier than that previously was supposed, most likely in the Mid-Miocene Instead of late Miocene as currently believed.Depto. de Geodinámica, Estratigrafía y PaleontologíaFac. de Ciencias GeológicasTRUEpu

Evaluation of the multispecies coalescent method to explore intra-Trypanosoma cruzi i relationships and genetic diversity

Chagas Disease is a zoonosis caused by the parasite Trypanosoma cruzi. Several high-resolution markers have subdivided T. cruzi taxon into at least seven lineages or Discrete Typing Units (DTUs) (TcI-TcVI and TcBat). Trypanosoma cruzi I is the most diverse and geographically widespread DTU. Recently a TcI genotype related to domestic cycles was proposed and named as TcIDOM. Herein, we combined traditional markers and housekeeping genes and applied a Multispecies Coalescent method to explore intra-TcI relationships, lineage boundaries and genetic diversity in a random set of isolates and DNA sequences retrieved from Genbank from different countries in the Americas. We found further evidence supporting TcIDOM as an independent and emerging genotype of TcI at least in Colombia and Venezuela. We also found evidence of high phylogenetic incongruence between parasite's gene trees (including introgression) and embedded species trees, and a lack of genetic structure among geography and hosts, illustrating the complex dynamics and epidemiology of TcI across the Americas. These findings provide novel insights into T. cruzi systematics and epidemiology and support the need to assess parasite diversity and lineage boundaries through hypothesis testing using different approaches to those traditionally employed, including the Bayesian Multispecies coalescent method. Copyright © Cambridge University Press 2019

Selection of Saccharomyces cerevisiae Strains Applied to the Production of Prieto Picudo Rosé Wines with a Different Aromatic Profile

The aim of this work was to select indigenous Saccharomyces cerevisiae strains based on a combination of genetic and aroma analyses to be used for inoculation in industrial fermentations and produce rosé wine with a different aromatic profile. A total of 118 indigenous strains of S. cerevisiae and one hybrid strain from five wineries and three different vintages were isolated from spontaneous microfermentations and genetically characterised according to the restriction fragment length polymorphism of their mitochondrial DNA (RFLP-mtDNA). From this group, 30 strains were subjected to phenotypic/oenological characterisation and, of these, nine were chosen as starters in wine fermentations due to their ability to ferment well and their appearance in consecutive vintages or in two or more wineries. Wines produced by these nine selected strains were aromatically and chemically characterised, revealing great differences in their sensory profiles. One of these strains (C9-I) showed the most complex aroma profile in the sensory characterisation, so it was selected to produce an industrial wine. A principal component analysis showed that the industrial wine produced was aromatically very different from several commercial wines produced by different wineries. In fact, their main aromatic attributes were not found in the commercial rosé wines selected for the sensory evaluation. The study shows that a combination of microbiological and chemical techniques can be an effective tool to improve the winemaking process to produce industrial wines with a distinctive organoleptic profile

Estudio básico del municipio de Silvania.

Se hace la caracterización de los sistemas de producción agrícolas, pecuarios y la estructura agraria prevalecientes en el municipio de Silvania en el departamento de Cundinamarca, con miras a la formulación de planes de desarrollo rural en la región. Se expone la metodología utilizada para el estudio, los aspectos biofísicos predominantes, los sistemas de explotación agrícola y pecuaria, la situación demográfica, de servicios públicos y las instituciones de apoyo a la producción. Se identifican los sistemas de producción pecuaria en los renglones de ovinos, porcinos, aves huevo y los sistemas agrícolas en cultivos de mora de castilla y arvej