340 research outputs found
Lyme borreliosis in dogs
La maladie de Lyme est caractĂ©risĂ©e chez le Chien par une arthrite induisant une boiterie, de lâanorexie,
de la fiÚvre, une léthargie, une adénomégalie et parfois une glomérulonéphrite fatale. En absence
de mise en évidence de Borrelia dans les prélÚvements, le diagnostic repose essentiellement sur un
faisceau dâarguments Ă©pidĂ©miologique, clinique, sĂ©rologique et thĂ©rapeutique, aprĂšs exclusion des
autres causes aboutissant au mĂȘme tableau clinique. La thĂ©rapeutique sâappuie sur une antibiothĂ©rapie
principalement Ă base de doxycycline. La prĂ©vention passe par lâutilisation dâacaricides contre
Ixodes spp., vecteurs des Borrelia et Ă©ventuellement lâadministration dâun vaccin.Lyme disease is characterized in dogs by fever, arthritis that induces lameness, anorexia, lethargy,
adenomegaly and sometimes fatal glomerulonephritis. In the absence of direct evidence of Borrelia
in the samples, the diagnosis is mainly based on a bundle of epidemiological, clinical, serological and
therapeutic arguments, after exclusion of the other causes leading to the same symptoms. Therapy is
based on antibiotic like doxycycline. Acaricides against Ixodes spp., vectors of Borrelia, are the main
tool for prevention. Vaccines are also possibly available
Transmission of Bartonella henselae by Ixodes ricinus
The causative agent of cat-scratch disease in humans can be transmitted by this tick through saliva
Bartonella spp. DNA Associated with Biting Flies from California
Bartonella DNA was investigated in 104 horn flies (Haematobia spp.), 60 stable flies (Stomoxys spp.), 11 deer flies (Chrysops spp.), and 11 horse flies (Tabanus spp.) collected on cattle in California. Partial sequencing indicated B. bovis DNA in the horn fly pool and B. henselae type M DNA in one stable fly
Genetic diversity of Anaplasma Phagocytophilum, the causative agent of granulocytic anaplasmosis, implications for epidemiology and control in france
Anaplasma phagocytophilum is a tick-borne bacterium and the etiologic agent of granulocytic anaplasmosis, an emerging disease that affects a wide range of mammals. In this paper, we present the recent knowledge gained from studies on the genetic diversity of this pathogen in France. Multilocus sequence analysis (MLSA) was used to characterize the genetic diversity of A. phagocytophilum in populations of French cattle, horses, dogs, and roe deer. MLSA was based on nine loci (ankA, msp4, groESL, typA, pled, gyrA, recG, polA, and an intergenic region). Phylogenic analysis revealed three genetic clusters of bacterial variants in domesticated animals. The two principal clusters included 98% of the bacterial genotypes found in cattle, which were only distantly related to those in roe deer. One cluster comprised only cattle genotypes, while the second contained genotypes from cattle, horses, and dogs. The third contained all roe deer genotypes and three cattle genotypes. These results suggest that roe deer do not contribute to the spread of A. phagocytophilum in cattle in France. A Multiple-Locus Variable number tandem repeat (VNTR) Analysis typing technique was developed for A. phagocytophilum. Five VNTRs were selected based on the HZ human-derived strain genome, and were tested on the Webster human-derived strain and on 123 DNA samples. This study confirmed that A. phagocytophilum from roe deer or domestic ruminants belong to two different clusters, while A. phagocytophilum from red deer and domestic ruminants locate within the same cluster, questioning the respective roles of roe vs red deer as reservoir hosts for domestic ruminant strains in Europe. The molecular techniques recently developed have great potential to provide detailed information on A. phagocytophilum isolates,improving both epidemiological and phylogenic investigations, thereby helping in the development of relevant prevention and control measures.A. phagocytophilum, bactĂ©rie transmise par les tiques, est responsable de lâanaplasmose granulocytaire, une maladie Ă©mergente qui infecte une large gamme de mammifĂšres dont lâhomme. Lâobjectif de cet article est de prĂ©senter les nouvelles connaissances acquises sur la diversitĂ© gĂ©nĂ©tique dâA. phagocytophilum chez diffĂ©rentes espĂšces dâhĂŽtes en France, afin de dĂ©terminer quelles espĂšces participent au mĂȘme cycle Ă©pidĂ©miologique. Une analyse par sĂ©quençage multi-locus (MLSA) a Ă©tĂ© effectuĂ©e dans des populations de bovins, chevaux, chiens et chevreuils. Trois groupes de gĂ©notypes infectant les bovins ont Ă©tĂ© identifiĂ©s. Les deux groupes principaux incluent 98% des gĂ©notypes bactĂ©riens trouvĂ©s chez les bovins et sont Ă©loignĂ©s de ceux des chevreuils. Un cluster ne comprenait que les gĂ©notypes de bovins, tandis que le second gĂ©notype contenant des bovins comprenait Ă©galement des chevaux et des chiens. Le troisiĂšme cluster contenait tous les gĂ©notypes de chevreuils et trois gĂ©notypes de bovins. Ces rĂ©sultats suggĂšrent que les chevreuils ne contribuent pas Ă la propagation dâA. phagocytophilum chez les bovins en France. Puis, une technique MLVA (Multiple Loci VNTR Analysis) a Ă©tĂ© dĂ©veloppĂ©e pour A. phagocytophilum. Cinq VNTR ont Ă©tĂ© sĂ©lectionnĂ©s sur la base du gĂ©nome de la souche dâorigine humaine HZ, et ont Ă©tĂ© testĂ©s sur 123 Ă©chantillons dâADN provenant dâanimaux domestiques ou sauvages. Cette Ă©tude a confirmĂ© que les souches dâA. phagocytophilum retrouvĂ©es chez les chevreuils et les ruminants domestiques appartiennent Ă deux groupes diffĂ©rents, alors que les souches identifiĂ©es chez les cerfs et les ruminants domestiques sont localisĂ©es dans le mĂȘme cluster. Ces rĂ©sultats remettent en question les rĂŽles respectifs des chevreuils et des cerfs comme hĂŽtes rĂ©servoirs pour les souches dâA. phagocytophilum de ruminants domestiques en Europe. Ces techniques molĂ©culaires ont un grand potentiel pour amĂ©liorer nos connaissances sur les cycles Ă©pidĂ©miologiques dâA. phagocytophilum, contribuant ainsi Ă lâĂ©laboration de mesures de prĂ©vention et de contrĂŽle pertinents
Endocarditis in Cattle Caused by Bartonella bovis
This study aimed to determine the role of Bartonella as an endocarditis agent in cattle. Bartonella bovis was identified by PCR, gene sequences analysis, and specific internal transcribed spacer amplicon product size in 2 bovine endocarditis cases with high antibody titers, which demonstrates that B. bovis is a pathogen for cattle
Bartonella rochalimae in Raccoons, Coyotes, and Red Foxes
To determine additional reservoirs for Bartonella rochalimae, we examined samples from several wildlife species. We isolated B. rochalimae from 1 red fox near Paris, France, and from 11 raccoons and 2 coyotes from California, USA. Co-infection with B. vinsonii subsp. berkhoffii was documented in 1 of the coyotes
Bartonella Spp. in Pets and Effect on Human Health
Pets represent a large reservoir for human infection
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