Identification of the mitochondrial receptor complex in Saccharomyces cerevisiae

Mitochondrial protein import involves the recognition of preproteins by receptors and their subsequent translocation across the outer membrane. In Neurospora crassa, the two import receptors, MOM19 and MOM72, were found in a complex with the general insertion protein, GIP (formed by MOM7, MOM8, MOM30 and MOM38) and MOM22. We isolated a complex out of S. cerevisiae mitochondria consisting of MOM38/ISP42, the receptor MOM72, and five new yeast proteins, the putative equivalents of N. crassa MOM7, MOM8, MOM19, MOM22 and MOM30. A receptor complex isolated out of yeast cells transformed with N. crassa MOM19 contained the N. crassa master receptor in addition to the yeast proteins. This demonstrates that the yeast complex is functional, and provides strong evidence that we also have identified the yeast MOM19

Интеграция цифровых технологий в процесс обучения иностранным языкам

В статье обсуждаются возможности цифровых технологий в преподавании иностранных языков. Обсуждаются возможные трудности и ограничения внедрения технологических средств обучения. Подчеркивается необходимость системной интеграции технологий в учебный процесс как при аудиторной так и при самостоятельной работе, а также важность адекватной методической поддержки используемых технологий

Breathing during REM and non-REM sleep: correlated versus uncorrelated behaviour

Abstract Healthy sleep can be characterized by several stages: deep sleep, light sleep, and REM sleep. Here we show that these sleep stages lead to di erent autonomic regulation of breathing. Using the detrended uctuation analysis up to the fourth order we ÿnd that breath-to-breath intervals and breath volumes separated by several breaths are long-range correlated during the REM stages and during wake states. In contrast, in the non-REM stages (deep sleep and light sleep), long-range correlations are absent. This behaviour is very similar to the correlation behaviour of the heart rate during the night and may be related to the phase synchronization between heartbeat and breathing found recently. We speculate that the di erences are caused by di erent cortically in uenced control of the autonomic nervous system

Catch-up-ESUS - follow-up in embolic stroke of undetermined source (ESUS) in a prospective, open-label, observational study: study protocol and initial baseline data

Introduction. So far there is no uniform, commonly accepted diagnostic and therapeutic algorithm for patients with embolic stroke of undetermined source (ESUS). Recent clinical trials on secondary stroke prevention in ESUS did not support the use of oral anticoagulation. As ESUS comprises heterogeneous subgroups including a wide age-range, concomitant patent foramen ovale (PFO), and variable probability for atrial fibrillation (AF), an individualised approach is urgently needed. This prospective registry study aims to provide initial data towards an individual, structured diagnostic and therapeutic approach in ESUS patients. Methods and analysis. The open-label, investigator-initiated, prospective, single-centre, observational registry study (Catch-up-ESUS) started in 01/2018. Consecutive ESUS patients ≥18 years who give informed consent are included and will be followed up for 3 years. Stratified by age <60 or ≥60 years, the patients are processed following a standardised diagnostic and treatment algorithm with an interdisciplinary design involving neurologists and cardiologists. Depending on the strata, patients receive a transesophageal echocardiogram; all patients receive an implantable cardiac monitor. Patients <60 years with PFO and without evidence of concomitant AF are planned for PFO closure within 6 months after stroke. The current diagnostic and therapeutic workup of ESUS patients requires improvement by both standardisation and a more individualised approach. Catch-up-ESUS will provide important data with respect to AF detection and PFO closure and will estimate stratified stroke recurrence rates after ESUS. Ethics and dissemination. The study has been approved by the responsible ethics committee at the Ludwig Maximilian University, Munich, Germany (project number 17–685). Catch-Up-ESUS is conducted in accordance with the Declaration of Helsinki. All patients will have to give written informed consent or, if unable to give consent themselves, their legal guardian will have to provide written informed consent for their participation. The first observation period of the registry study is 1 year, followed by the first publication of the results including follow-up of the patients. Further publications will be considered according the predefined individual follow-up dates of the stroke patients up to 36 months

Preliminary morpho-agronomic characterization of cumari hot peppers

A pimenta cumari, Capsicum baccatum var. praetermissum e C. baccatum var. baccatum, é uma espécie semidomesticada, endêmica das regiões Sudeste e Centro-Oeste do país. Apesar da sua importância econômica, principalmente para a agricultura familiar, e da sua ampla variabilidade, ainda não existe uma cultivar dessa espécie disponível no mercado brasileiro. Este trabalho teve como objetivo avaliar acessos de pimenta cumari do Banco de Germoplasma de Capsicum da Embrapa Hortaliças com base em características morfoagronômicas e identificar acessos promissores que possam dar origem a um programa de melhoramento genético. Verificou-se a existência de grande variabilidade entre os acessos analisados, tendo sido encontradas diferenças para a maioria das variáveis. O material mais precoce, CNPH 4065, floresceu aos 81 dias após a semeadura e o mais tardio, CNPH 2957, aos 126 dias. O número de flores por axila variou de 1 a 3. Já em relação à pilosidade, a quantidade de plantas muito pilosas foi de 6,25%; pilosidade moderada 6,25%; pouca pilosidade 12,50% e sem pilosidade 75,00%. A estimativa da produtividade variou de 8 a 340 gramas por planta e a incidência de viroses indicou o PepYMV como o mais frequente, tendo sido encontrado em 33% das plantas analisadas. Os acessos CNPH 4065, CNPH 3824, CNPH 3993, CNPH 2964 e CNPH 2948 foram considerados promissores para o uso em programa de melhoramento.The cumari hot pepper, Capsicum baccatum var. praetermissum and C. baccatum var. baccatum, is a semi-domesticated species, endemic to the Southeast and Midwest regions of Brazil. Despite its economic importance, especially for small farmers in family agriculture systems, no cumari cultivars are available in the Brazilian market. This research aimed to evaluate 16 cumari accessions of the Capsicum Germplasm Bank of Embrapa Vegetables based on morpho-agronomic features, and identify promising accessions for further breeding programs. Genetic variability was found among accessions and differences were observed for the majority of the characteristics. The earliest genotype, CNPH 4065, set flowers 81 days after sowing and the latest, CNPH 2957, at 126 days. The number of flowers per axil ranged from 1 to 3. Pubescence, a relevant plant characteristic associated to insect resistance was found as follows: highly pubescent plants 6.25%; moderately pubescent 6.25%; almost glabrous 12.50%; and glabrous 75.00%. Yield was estimated in between 8 grams/plant and 340 grams/plant. PepYMV (33% of the total number of plants) was the most prevalent virus infecting cumari hot peppers in the field. Five accessions (CNPH 4065; CNPH 3824; CNPH 3993; CNPH 2964; CNPH 2948) were considered as promising to be used in cumari breeding programs

Les Houches 2015: Physics at TeV Colliders Standard Model Working Group Report

This Report summarizes the proceedings of the 2015 Les Houches workshop on Physics at TeV Colliders. Session 1 dealt with (I) new developments relevant for high precision Standard Model calculations, (II) the new PDF4LHC parton distributions, (III) issues in the theoretical description of the production of Standard Model Higgs bosons and how to relate experimental measurements, (IV) a host of phenomenological studies essential for comparing LHC data from Run I with theoretical predictions and projections for future measurements in Run II, and (V) new developments in Monte Carlo event generators.Comment: Proceedings of the Standard Model Working Group of the 2015 Les Houches Workshop, Physics at TeV Colliders, Les Houches 1-19 June 2015. 227 page

Associations between the 17q21 region and allergic rhinitis in 5 birth cohorts

Non peer reviewe

CLIC: a Two-Beam Multi-TeV e±e\pm Linear Collider

The CLIC study of a high-energy (0.5 - 5 TeV), high-luminosity (1034 - 1035 cm-2 sec-1) e+e- linear collider is presented. Beam acceleration using high frequency (30 GHz) normal-conducting structures operating at high accelerating fields (150 MV/m) significantly reduces the length and, in consequence, the cost of the linac. Using parameters derived from general scaling laws for linear colliders, the beam stability is shown to be similar to lower frequency designs in spite of the strong wake-field dependency on frequency. A new cost-effective and efficient drive beam generation scheme for RF power production by the so-called "Two-Beam Acceleration" method is described. It uses a thermionic gun and a fully-loaded normal-conducting linac operating at low frequency (937 MHz) to generate and accelerate the drive beam bunches, and RF multiplication by funnelling in compressor rings to produce the desired bunch structure. Recent 30 GHz hardware developments and CLIC Test Facility (CTF) results are described