Multiple-Locus Variable Number Tandem Repeat Analysis of Staphylococcus Aureus: Comparison with Pulsed-Field Gel Electrophoresis and spa-Typing

(MRSA) is required to study the routes and rates of transmission of this pathogen. Currently available typing techniques are either resource-intensive or have limited discriminatory ability. Multiple-locus variable number tandem repeat analysis (MLVA) may provide an alternative high throughput molecular typing tool with high epidemiological resolution.-sequence typing and PFGE, at the MLVA complex level with group separation values of 95.1% and 89.2%. MLVA could not discriminate between pig-related MRSA strains isolated from humans and pigs, corroborating the high degree of relationship. MLVA was also superior in the grouping of MRSA isolates previously assigned to temporal-spatial clusters with indistinguishable SpaTypes, demonstrating its enhanced epidemiological usefulness. that yields discrete and unambiguous data that can be used to assign biological meaningful genotypes and complexes and can be used for interlaboratory comparisons in network accessible databases. Results suggest that MLVA offsets the disadvantages of other high discriminatory typing approaches and represents a promising tool for hospital, national and international molecular epidemiology

Towards maximum energy efficiency of carrier-injection-based silicon photonics

We present carrier-injection-based photonic switches, engineered for optical pulse distribution with maximum energy efficiency. We apply small-signal analysis and for the first time large-signal modelling to methodically optimize the switches for minimum energy consumption and to classify the electronic contributions from resistance, capacitance, and diode. We present optimized electronic switch activation, which yields a sixfold reduction in energy consumption and we show how static power consumption becomes a negligible factor for optical pulse switching. We demonstrate that with adjusted phase shifter dimensions, MZI-based switches can operate with additional 50% enhanced energy efficiency with down to 4 pJ per switching operation. We show even further efficiency improvement using ring-based designs, allowing an additional improvement of 50% in energy efficiency and we discuss the trade-off between efficiency and optical bandwidth associated to the Q-factor. We benchmark carrier-injection-based switches together with comparable technologies of the silicon photonics platform and identify carrier-injection to be the most suitable technology for pulse switching applications

Integrated recirculating optical hybrid silicon buffers

We present our work on fully integrated hybrid silicon optical buffers capable of holding 40 byte packets at 40 Gb/s. These devices consist of low loss silicon waveguides and cascaded amplifiers to overcome passive losses in a 1.1 m long delay line. Since cascading multiple gain elements leads to ASE (noise) accumulation, reshaping elements in the form of saturable absorbers are integrated in the delay. Noise filtering in the buffer is investigated by simulating the eye diagram for a delay line with 1R regenerators and comparing it to that of a 2R regenerator. Finally, preliminary experimental data from the optical buffer is shown

A software product certification model

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