Efficient fetal-maternal ECG signal separation from two channel maternal abdominal ECG via diffusion-based channel selection

There is a need for affordable, widely deployable maternal-fetal ECG monitors to improve maternal and fetal health during pregnancy and delivery. Based on the diffusion-based channel selection, here we present the mathematical formalism and clinical validation of an algorithm capable of accurate separation of maternal and fetal ECG from a two channel signal acquired over maternal abdomen

Visual Search for Galaxies near the Northern Crossing of the Supergalactic plane by the Milky Way

We have visually examined twelve Palomar red Plates for galaxies at low Galactic latitude b, where the Supergalactic Plane (SGP) is crossed by the Galactic Plane (GP), at Galactic longitude l ~135 degrees. The catalogue consists of 2575 galaxy candidates, of which 462 have major axis diameters d >= 0.8 arc min (uncorrected for extinction). Galaxy candidates can be identified down to |b| ~ 0 degrees. One of our galaxy candidates (J24 = Dwingeloo 1) has recently been discovered independently in 21cm by Kraan-Korteweg et al. (1994) as a nearby galaxy. Comparisons with the structures seen in the IRAS and UGC catalogues are made. We compare the success rate of identifying galaxies using the IRAS Point Source Catalogue under different colour selection criteria. The criteria that require both the 60 micron and 100 micron fluxes to be of high quality, have the highest probability of selecting a galaxy (with d >= 0.6 arc min), but at the expense of selecting a smaller number of galaxies in total.Comment: uuencoded compressed postscript, without figures. The figures are available at http://www.ast.cam.ac.uk/preprint/PrePrint.htm

The puzzlingly large Ca II triplet absorption in dwarf elliptical galaxies

We present central CaT, PaT, and CaT* indices for a sample of fifteen dwarf elliptical galaxies (dEs). Twelve of these have CaT* ~ 7 A and extend the negative correlation between the CaT* index and central velocity dispersion sigma, which was derived for bright ellipticals (Es), down to 20 < sigma < 55 km/s. For five dEs we have independent age and metallicity estimates. Four of these have CaT* ~ 7 A, much higher than expected from their low metallicities (-1.5 < [Z/H] < -0.5). The observed anti-correlation of CaT* as a function of sigma or Z is in flagrant disagreement with theory. We discuss some of the amendments that have been proposed to bring the theoretical predictions into agreement with the observed CaT*-values of bright Es and how they can be extended to incorporate also the observed CaT*-values of dEs. Moreover, 3 dEs in our sample have CaT* ~ 5 A, as would be expected for metal-poor stellar systems. Any theory for dE evolution will have to be able to explain the co-existence of low-CaT* and high-CaT* dEs at a given mean metallicity. This could be the first direct evidence that the dE population is not homogeneous, and that different evolutionary paths led to morphologically and kinematically similar but chemically distinct objects.Comment: 4 pages, 3 figures, accepted for publication in ApJ Letter

Biomolecular imaging and electronic damage using X-ray free-electron lasers

Proposals to determine biomolecular structures from diffraction experiments using femtosecond X-ray free-electron laser (XFEL) pulses involve a conflict between the incident brightness required to achieve diffraction-limited atomic resolution and the electronic and structural damage induced by the illumination. Here we show that previous estimates of the conditions under which biomolecular structures may be obtained in this manner are unduly restrictive, because they are based on a coherent diffraction model that is not appropriate to the proposed interaction conditions. A more detailed imaging model derived from optical coherence theory and quantum electrodynamics is shown to be far more tolerant of electronic damage. The nuclear density is employed as the principal descriptor of molecular structure. The foundations of the approach may also be used to characterize electrodynamical processes by performing scattering experiments on complex molecules of known structure.Comment: 16 pages, 2 figure

A novel method, based on lithium sulfate precipitation for purification of chicken egg yolk immunoglobulin Y, applied to immunospecific antibodies against Sendai virus.

Egg-laying hens were immunized with Sendai virus (SV) that had been grown in chicken embryos. The  titres of immunospecific SV antibodies varied from log212 to log216 during the 5-month immunization  period and total immunoglobulin Y (IgY) concentrations varied from 1.6 to 4.0 mg per ml of egg yolk.  Two IgY purification methods based on salt precipitation using lithium sulfate or sodium citrate were  developed. These methods were compared with two other purification methods based on polyethylene glycol  (PEG) precipitation and chloroform extraction, respectively in terms of yield, total protein content, IgY  concentration and immunospecific anti Sendai IgY activity. The total protein and IgY contents when purified  by chloroform were 1.4-2.8 times and 1.3-2.3 times higher, respectively than in corresponding preparations  purified by the other methods. However, the proportion of nonsense proteins was approximately  10% higher in the IgY preparation purified by chloroform than in those purified by salt precipitation. The  immunospecific IgY activity recorded in the preparations from the new salting out methods was lower  compared with the PEG and chloroform purification methods. However, the purity analysis of IgY by sodium  dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that IgY purified with  lithium sulfate contained only two major components with molecular weights of 40 kDa and 66 kDa and  one minor protein component of 45 kDa. By contrast, IgY extracted with ammonium sulfate, which is a  classical method used to obtain purified IgY, contained two major protein of 40 kDa and 66 kDa and at  least three less intense protein bands corresponding to proteins of molecular weights 31.4 kDa, 33.5 kDa  and 45 kDa. The results indicate that the purification of IgY by lithium sulfate results in very pure IgY in  high quantities (94% +/- 5% of total egg yolk protein).

An Observational Limit on the Dwarf Galaxy Population of the Local Group

We present the results of an all-sky, deep optical survey for faint Local Group dwarf galaxies. Candidate objects were selected from the second Palomar survey (POSS-II) and ESO/SRC survey plates and follow-up observations performed to determine whether they were indeed overlooked members of the Local Group. Only two galaxies (Antlia and Cetus) were discovered this way out of 206 candidates. Based on internal and external comparisons, we estimate that our visual survey is more than 77% complete for objects larger than one arc minute in size and with a surface brightness greater than an extremely faint limit over the 72% of the sky not obstructed by the Milky Way. Our limit of sensitivity cannot be calculated exactly, but is certainly fainter than 25 magnitudes per square arc second in R, probably 25.5 and possibly approaching 26. We conclude that there are at most one or two Local Group dwarf galaxies fitting our observational criteria still undiscovered in the clear part of the sky, and a roughly a dozen hidden behind the Milky Way. Our work places the "missing satellite problem" on a firm quantitative observational basis. We present detailed data on all our candidates, including surface brightness measurements.Comment: 58 pages in AJ manuscript format; some figures at slightly reduced quality; accepted by the Astronomical Journa

Storage and perpendicular retrieving of two-dimensional pulses in electromagnetically induced transparency media

Propagation of two dimensional pulses in electromagnetically induced tranparency media in the case of perpendicular storing and retrieving pulses has been analyzed. It has been shown that propagation control of the pulses in optically thick media can be used for producing interchange between pulse time-shape and intensity profile distribution. A simple obvious analytical solution for the retrieved new field has been obtained.Comment: 6 pages, 4 figure

Penetration of the Interplanetary Magnetic Field B(sub y) into Earth's Plasma Sheet

There has been considerable recent interest in the relationship between the cross-tail magnetic field component B(sub y) and tail dynamics. The purpose of this paper is to give an overall description of the penetration of the interplanetary magnetic field (IMF) B(sub y) into the near-Earth plasma sheet. We show that plasma sheet B(sub y) may be generated by the differential shear motion of field lines and enhanced by flux tube compression. The latter mechanism leads to a B(sub y) analogue of the pressure-balance inconsistency as flux tubes move from the far tail toward the Earth. The growth of B(sub y), however, may be limited by the dawn-dusk asymmetry in the shear velocity as a result of plasma sheet tilting. B(sub y) penetration into the plasma sheet implies field-aligned currents flowing between hemispheres. These currents together with the IMF B(sub y) related mantle field-aligned currents effectively shield the lobe from the IMF B(sub y)

Maternal serum levels of pregnancy—associated murine-l (PAMP—l) during pregnancy in the rabbit

An ELISA was developed to measure for the first time serum levels of Pregnancy—Associated Murine Protein- 1 (PAMP—l) throughout pregnancy in the rabbit. In rodents serum levels of PAMP-l are regulated by growth hormone Unlike the pregnancy-assoeiated rise in serum levels in pregnant mice and rats, PAMP-l did not increase significantly during pregnancy in the rabbit

Influence of husbandry procedures on mouse locomotor activity

The currently used raL models of obesity and diabetes are derived from either Zucker or from Koletsky rats. Recently, we identified a spontaneous obese rat from out Wistar colony which is maintained as an inbred stock for the past 75 years. Initially, one of the male progeny in a litter was observed to have abnormal body weight for its age. The parents of this ral were identified, the progeny selectively bred, and a colony has been developed. This is designated as WNIN-0b. The colony is maintained by mating heterozygous animals (+/ob), as the homozygous (ob/ob) were found to be infertile. The trait is carried as an autosomal recessive mutation and the colony is currenfly in F7 generation.Obesity is visible in these mutants around 35 days of age. They are hyperphagic and reach a body weight of 500—600 g by 105 days of age. “Kinky” tail is characteristic of this mutant and this is visible around 50-60 days. Sexual maturity is delayed in female obese mutants, as judged by the day of vaginal opening. The animals are cuglyccmic and show hyperinsulinaemia, hypertriglyceridaemia, arid hypercholesterolemia. Another mutant showing hyperglycemia is also obtained fromthe obese colony. Unlike earlier models which are essentially derived from a randomAbred stock, this is the first report of a rat obese model, developed spontaneously from an inbred strain