A novel NGS library preparation method to characterize native termini of fragmented DNA.

Biological and chemical DNA fragmentation generates DNA molecules with a variety of termini, including blunt ends and single-stranded overhangs. We have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragmented DNA, information traditionally lost in standard NGS library preparation methods. Here we describe the XACTLY method, showcase its sensitivity and specificity, and demonstrate its utility in in vitro experiments. The XACTLY assay is able to report relative abundances of all lengths and types (5' and 3') of single-stranded overhangs, if present, on each DNA fragment with an overall accuracy between 80-90%. In addition, XACTLY retains the sequence of each native DNA molecule after fragmentation and can capture the genomic landscape of cleavage events at single nucleotide resolution. The XACTLY assay can be applied as a novel research and discovery tool for fragmentation analyses and in cell-free DNA

Examination of the 1968-69 Urban Indian Hearings Held by the National Council on Indian Opportunity.

Training Center for Community Programs

Indian Americans in Duluth: A Summary and Analysis of Recent Research.

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Education-Related Preferences and Characteristics of College-Aspiring Urban Indian Teenagers: A Preliminary Report.

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Social Programs and Political Styles of Minneapolis Indians: An Interim Report.

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Attitude of Minneapolis Agency Personnel Toward Urban Indians.

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Indian Americans in Chicago.

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Indian Employment in Minneapolis.

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Attitudes and Characteristics of Selected Wisconsin Indians.

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Rural and City Indians in Minnesota Prisons.

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