Attack of \u3ci\u3eUrophora Quadrifasciata\u3c/i\u3e (Meig.) (Diiptera: Tephritidae) A Biological Control Agent for Spotted Knapweed (\u3ci\u3eCentaurea Maculosa\u3c/i\u3e Lamarck) and Diffuse Knapweed (\u3ci\u3eC. Diffusa\u3c/i\u3e Lamarck) (Asteraceae) by a Parasitoid, \u3ci\u3ePteromalus\u3c/i\u3e Sp. (Hymenoptera: Pteromalidae) in Michigan

Urophora quadrifasciata (Meig.) a seedhead fly released in North America for biological control of Centaurea maculosa and C. diffusa is parasitized by a Pteromalus sp. Parasitism up to 60% of U. quadrifasciata was found in samples of seed heads of C. maculosa and C. diffusa collected from 54 of the 59 counties sampled in Michigan and in one sample of C. maculosa seed heads from Hennepin County, Minnesota. Parasitism of U. quadrifasciata has rarely been reported

\u3ci\u3eUrophora Affinis\u3c/i\u3e and \u3ci\u3eU. Quadrifasciata\u3c/i\u3e (Diptera: Tephritidae) Released and Monitored by USDA, APHIS, PPQ as Biological Control Agents of Spotted and Diffuse Knapweed

USDA, APHIS, PPQ has distributed the seedhead gall flies Urophora affinis and U. quadrifasciata (Diptera: Tephritidae) as classical biological agents of the introduced weeds spotted and diffuse knapweed (Centaurea maculosa and C. diffusa, respectively) (Asteraceae) in the United States. From 1987 to 1996, Urophora spp. have been released in 97 counties in 14 midwestern and western states. Established populations of U. affinis and U. quadrifasciata are confirmed in 85 and 95 counties, respectively, among all 14 states. These include the first reports of successful establishment of Urophora spp. in Arizona (two counties), Colorado (eight counties), Michigan (one county), Minnesota (six counties), Nebraska (four counties), Nevada (two counties), North Dakota (one county), South Dakota (four coun­ties), Utah (three counties), and Wisconsin (two counties). The first confirmed establishment of U. quadrifasciata in Indiana and Michigan is also reported

More Grain Storage On Iowa Farms

Many Iowa farmers will be putting up new grain-storage structures this summer and fall. There are some special incentives available this year. Here are some of the things to consider when thinking of building storage space

\u3ci\u3eUrophora Affinis\u3c/i\u3e and \u3ci\u3eU. Quadrifasciata\u3c/i\u3e (Diptera: Tephritidae) Released and Monitored by USDA, APHIS, PPQ as Biological Control Agents of Spotted and Diffuse Knapweed

USDA, APHIS, PPQ has distributed the seedhead gall flies Urophora affinis and U. quadrifasciata (Diptera: Tephritidae) as classical biological agents of the introduced weeds spotted and diffuse knapweed (Centaurea maculosa and C. diffusa, respectively) (Asteraceae) in the United States. From 1987 to 1996, Urophora spp. have been released in 97 counties in 14 midwestern and western states. Established populations of U. affinis and U. quadrifasciata are confirmed in 85 and 95 counties, respectively, among all 14 states. These include the first reports of successful establishment of Urophora spp. in Arizona (two counties), Colorado (eight counties), Michigan (one county), Minnesota (six counties), Nebraska (four counties), Nevada (two counties), North Dakota (one county), South Dakota (four coun­ties), Utah (three counties), and Wisconsin (two counties). The first confirmed establishment of U. quadrifasciata in Indiana and Michigan is also reported

Tropical forcing of the Summer East Atlantic pattern

The Summer East Atlantic (SEA) mode is the second dominant mode of summer low-frequency variability in the Euro-Atlantic region. Using reanalysis data, we show that SEA-related circulation anomalies significantly influence temperatures and precipitation over Europe. We present evidence that part of the interannual SEA variability is forced by diabatic heating anomalies of opposing signs in the tropical Pacific and Caribbean that induce an extratropical Rossby wave train. This precipitation dipole is related to SST anomalies characteristic of the developing ENSO phases. Seasonal hindcast experiments forced with observed sea surface temperatures (SST) exhibit skill at capturing the interannual SEA variability corroborating the proposed mechanism and highlighting the possibility for improved prediction of boreal summer variability. Our results indicate that tropical forcing of the SEA likely played a role in the dynamics of the 2015 European heat wave

Theory for polymer coils with necklaces of micelles

If many micelles adsorb onto the same polymer molecule then they are said to form a necklace. A minimal model of such a necklace is proposed and shown to be almost equivalent to a 1-dimensional fluid with nearest-neighbour interactions. The thermodynamic functions of this fluid are obtained and then used to predict the change in the critical micellar concentration of the surfactant in the presence of the polymer. If the amount of polymer is not too large there are two critical micellar concentrations, one for micelles in necklaces and one for free micelles.Comment: 12 pages, 5 figure

Polymerase chain reaction detection of avipox and avian papillomavirus in naturally infected wild birds: comparisons of blood, swab and tissue samples

Avian poxvirus (avipox) is widely reported from avian species, causing cutaneous or mucosal lesions. Mortality rates of up to 100% are recorded in some hosts. Three major avipox clades are recognized. Several diagnostic techniques have been reported, with molecular techniques used only recently. Avipox has been reported from 278 different avian species, but only 111 of these involved sequence and/or strain identification. Collecting samples from wild birds is challenging as only few wild bird individuals or species may be symptomatic. Also, sampling regimes are tightly regulated and the most efficient sampling method, whole bird collection, is ethically challenging. In this study, three alternative sampling techniques (blood, cutaneous swabs and tissue biopsies) from symptomatic wild birds were examined. Polymerase chain reaction was used to detect avipoxvirus and avian papillomavirus (which also induces cutaneous lesions in birds). Four out of 14 tissue samples were positive but all 29 blood samples and 22 swab samples were negative for papillomavirus. All 29 blood samples were negative but 6/22 swabs and 9/14 tissue samples were avipox-positive. The difference between the numbers of positives generated from tissue samples and from swabs was not significant. The difference in the avipox-positive specimens in paired swab (4/6) and tissue samples (6/6) was also not significant. These results therefore do not show the superiority of swab or tissue samples over each other. However, both swab (6/22) and tissue (8/9) samples yielded significantly more avipox-positive cases than blood samples, which are therefore not recommended for sampling these viruses.The authors thank bird ringers from Alula and Monticola, especially Alfredo Ortega and Chechu Aguirre, for help with the capture and ringing of birds, which made this project possible. Thanks to Alvaro Ramírez for samples. This research was funded by the Ministerio de Ciencia e Innovación, Spain (grant number: CGL2010-15734/BOS). R.A.J.W. was supported by the Programa Internacional de Captación de Talento (PICATA) de Moncloa Campus de Excelencia Internacional while writing the manuscript

The microaerophilic microbiota of de-novo paediatric inflammatory bowel disease: the BISCUIT study

<p>Introduction: Children presenting for the first time with inflammatory bowel disease (IBD) offer a unique opportunity to study aetiological agents before the confounders of treatment. Microaerophilic bacteria can exploit the ecological niche of the intestinal epithelium; Helicobacter and Campylobacter are previously implicated in IBD pathogenesis. We set out to study these and other microaerophilic bacteria in de-novo paediatric IBD.</p> <p>Patients and Methods: 100 children undergoing colonoscopy were recruited including 44 treatment naïve de-novo IBD patients and 42 with normal colons. Colonic biopsies were subjected to microaerophilic culture with Gram-negative isolates then identified by sequencing. Biopsies were also PCR screened for the specific microaerophilic bacterial groups: Helicobacteraceae, Campylobacteraceae and Sutterella wadsworthensis.</p> <p>Results: 129 Gram-negative microaerophilic bacterial isolates were identified from 10 genera. The most frequently cultured was S. wadsworthensis (32 distinct isolates). Unusual Campylobacter were isolated from 8 subjects (including 3 C. concisus, 1 C. curvus, 1 C. lari, 1 C. rectus, 3 C. showae). No Helicobacter were cultured. When comparing IBD vs. normal colon control by PCR the prevalence figures were not significantly different (Helicobacter 11% vs. 12%, p = 1.00; Campylobacter 75% vs. 76%, p = 1.00; S. wadsworthensis 82% vs. 71%, p = 0.312).</p> <p>Conclusions: This study offers a comprehensive overview of the microaerophilic microbiota of the paediatric colon including at IBD onset. Campylobacter appear to be surprisingly common, are not more strongly associated with IBD and can be isolated from around 8% of paediatric colonic biopsies. S. wadsworthensis appears to be a common commensal. Helicobacter species are relatively rare in the paediatric colon.</p&gt