Surat Keputusan Dekan Fakultas Kedokteran Hewan Universitas Airlangga tentang Tim Pembina Kegiatan Mahasiswa Fakultas Kedokteran Hewan Universitas Airlangga Tahun 2023

Kumpulan Surat SK Hani Plumeriastuti yang dipergunakan untuk Keperluan Kenaikan Pangkat dan lain-lai

Keputusan Dekan Fakultas Kedokteran Hewan Universitas Airlangga tentang Promotor dan Ko-promotor Mahasiswa Program Studi S3 Sains Veteriner fakultas Kedokteran Hewan Universitas Airlangga Januari 2023 An. Yusrizal Akmal

Kumpulan Surat SK Hani Plumeriastuti yang dipergunakan untuk Keperluan Kenaikan Pangkat dan lain-lai

Inhibition of Apoptosis in Retinal of Newborn Mice Due to Congenital Toxoplasmosis

Toxoplasma gondii infection in pregnant women cause defects in the newborn, such as hydrocephalus and eye damaged, even blindness. Histologically damage due to congenital infection of T. gondii need to be examined. Twenty pregnant mice were divided into two groups which are the treatment group and the control group. Each mouse in treatment group was infected with 10 takizoit by intraperitoneal. Each of the newborn were sacrified, their head were taken and their eye tissue were fixed in 10% of buffered formalin and the histological sample were made in HE and TUNEL staining. The result showed that the retina of the eye of the newborn from infected mice damage. The damages include: hemorrhage, infected retinal cells, eye growth inhibition and decreased of apoptosis index of the retina cells.  Keywords: Apoptosis in retinal, Newborn, Congenital Toxoplasmosis, Ocular Toxoplasmosi

THE DILATATION OF BRAIN VENTRICLE DUE TO CONGENITAL TOXOPLASMOSIS IN MICE CORRELATED WITH APOPTOSIS BUT NOT WITH TRANSFORMING GROWTH FACTOR BETA

This study aimed to determine the occurences of mice brain ventricles dilatation that congenitally infected with Toxoplasma gondii (T. gondii) as a marker of hydrocephalus and cellular changes in the brain. A total of twenty pregnant mice (11.5 days pregnacy) were divided into 2 groups, which were control (P1) group and treatment (P2) group. The mice in the treatment group were infected with 101 tachyzoites of T. gondii. All mice were maintained until delivery. The newborn mice were sacrificed and their brain were removed and fixed in 10% buffered formalin to prepare histology slides with HE staining for observation of ventricular width, TUNEL assay for apoptosis observation, and immunohistochemistry for the expression of transforming growth factor beta (TGF-β) observations. The data were analyzed using t test and linear regression. The results showed that ventricular width and apoptosis index significantly increased (P0.01) in the treatment group compared to control group, but there was no difference in the expression of TGF-β (P0.05) in both groups. Dilatation of ventricle correlated with the apoptotic index of brain cells but did not correlated with the expression of TGF-β

Protection of Dayak Onion Tuber Extract (Eleutherine Palmifolia) Against Kidney Histopathological Appearence of Albino Male Rat Strain Wistar which was Induced by Alloxan

The purpose of this study was to know the effect of Dayak onion tuber extract (Eleutherine palmifolia) given by per oral in lowering levels of histophatology damage kidney of albino male rat (Rattus norvegicus) strain Wistar which was induced by alloxan. Animals which were used in this research were 24 white male rats (Rattus norvegicus) strain Wistar, and then divided into 6 groups. The negative control group K (-) were given with aquabidest and CMC-Na 1% during the therapy period, the positive control group K (+) were given with alloxan 110 mg / kgbw, the group of drug control K (O) were given with alloxan and oral therapy  with metformin 9 mg / 200g bw / day, the treatment group 1 (P1) were given with alloxan and per oral therapy with extract of  Dayak onion tuber 100 mg / kgbw, the treatment group 2 (P2) were given with alloxan and per oral therapy with extract of Dayak onion tuber 200 mg / kgbw and the treatment group 3 (P3) were given with alloxan and per oral therapy with extract of Dayak onion tuber 400 mg / kgbw. The therapy was given for 14 days, then the animals were sacrificed with ketamine and then its kidney was taken for examination of hisphatology in kidney. Observations based on their depiction of renal histopathology tubular degeneration and necrosis, glomerular necrosis, intestitial infiltration and glomerular sclerosis. Data obtained from the scoring of histopathological appearence albino rat kidneys were analyzed by test Kruskal-Wallis and if there is a real difference followed by Mann-Whitney test using SPSS 20.0 for windows. The results showed that the extract of Dayak onion tuber 400 mg/ kgbw  can reduce the degree of kidney damage in albino male rat exposed to alloxan significantly.  Key words: Eleutherine palmifolia, alloxan, kidney, histhopatholog

Teratogenic Effect of Congenital Toxoplasmosis in Chicken Embryo

This research is designed to observe the teratogenic effect of Toxoplasma gondii infection in chick embryos, based on the number of somites, embryo length and the development of embryonic brain vesicles. Methods in the research: Chicken eggs were infected with 1 x 103 tachyzoites of T. gondii. The eggs were incubated in eggs hatching box. Observation of somite performed on embryonated eggs 24 hours after incubation and the embryonic development of vesicles performed 72 hours after incubation then the length of each embryo were measured. Results: Revealed that there was a significant difference in the number of somites (p < 0.1), T. gondii infection reduced the number of somites. While in the number of brain vesicles in 3 - days old chicken embryos, although there was no significant difference, the size declining emerged. The length of the embryos both at 24 or 72 hours old showed that T. gondii infection reduced the length (p < 0.1). Conclusions: T. gondii infection influences the development of chicken embryos in the declining of length and the decreasing of somite embryo number. Keywords: IGF-I crossbreed mare serum pregnant; Follicle; Mus musculu

Karakterisasi Molekular Gen Penyandi SodC Pasteurella multocida yang Diisolasi dari Kerbau Asal Nusa Tenggara Timur

Pasteurella multocida tipe B:2 merupakan bakteri yang menyebabkan penyakit Septicemia Epizooticae pada ruminansia, khususnya kerbau dan sapi. Tujuan dari penelitian ini adalah untuk menentukan gen SodC dari Pasteurella multocida tipe B:2 yang berperan dalam virulensi bakteri sebagai antifagositosis. Isolat Pasteurella multocida tipe B:2 ini diperoleh dari BBVet Denpasar, Bali yang berasal dari Nusa Tenggara Timur (NTT) Indonesia. Sampel ditanam pada Blood Agar (BA). Koloni yang terpisah dilihat secara makroskopik (bening, bulat dan berbau manis), mikroskopik (bipolar dan negatif Gram), uji biokimia serta diperiksa menggunakan polymerase chain reaction (PCR). Hasil positif pada uji PCR apabila menunjukkan pita pada posisi 235 basepair (bp). Penelitian ini dilanjutkan dengan uji sekuensing untuk mendapatkan urutan nukleotida dari gen penyandi SodC Pasteurella multocida dan juga homologi isolate referensi dari Genbank atau NCBI. Hasil blast pada NCBI menyatakan bahwa gen penyandi SodC dari isolat penelitian memiliki nukleotida yang sama dengan strain 4407 (97% ), Strain Chinese 9N (98%), strain BS168 (97%), strain EB168 China (97%), strain subsp. multocida CIRMBP-0884 Prancis (97%), dan strain subsp. multocida RCAD0259 Cina (96%

Revitalisasi Fungsi Sel Beta Pankreas Tikus Putih Penderita Diabetes Militus Buatan Dengan Penggunaan Simplisia Kayu Manis (Cinnamomum Burmanii) Dan Kembang Bulan (Tithonia Diversifolia)

Penyakit Diabetes Melitus (DM) termasuk salah satu penyakit metabolik dengankadar glukosa darah tinggi (hiperglikemia). Diabetes Melitus (DM) merupakan suatu sindrom yang mengganggu proses metabolisme karbohidrat, lemak dan protein, sehingga mengakibatkan penurunan sekresi insulin atau penurunan sensitivitas jaringan terhadap insulin. Hiperglikemia disebabkan oleh beberapa faktor, yaitu penurunan sekresi insulin yang dihasilkan oleh sel beta pankreas, penurunan penggunaan glukosa di otot dan peningkatan produksi glukosa. Penyakit Diabetes Melitus (DM) ini bersifat kronis dan penderitanya terjadi pada segala umur. Tanaman kembang bulan dan kayu manis merupakan tumbuhan yang mampu mengatasi penyakit DM. Kedua tanaman mengandung senyawa aktif yang mampu merevitalisasi sel beta pankreas. Tanaman ini mengandung zat aktif yang tennasuk golongan minyak atsiri, alkaloid, flavonoid, saponin, triterpenoid dan polifenol. Daun kembang bulan sedikitnya mengandung 12 senyawa terpenoid dan 14 senyawa flavonoid. Senyawa aktif polifenol yang dimiliki oleh tanaman ini berperan sebagai antioksidan yang mampu menghambat reaksi oksidasi radikal bebas aloksan melalui mekanisme penangkapan radikal dengan eara menyumbangkan $4tu elektron pada elektron yang tidak berpasangan dalam radikal bebas sehingga banyaknya radikal bebas menjadi berkurang. Diduga tereduksinya radikal bebas aloksan oleh senyawa aktif polifenol pada struktur pankreas, berdampak pada perbaikan struktur pankreas dan respon immun limpa menurun sehingga terjadi normalisasi limpa. Tujuan dari penelitian ini adalah untuk mengetahui kemampuan ekstrak batang kayu manis (Cinnamomum burmanii) dan ekstrak daun kembang bulan (Tithonia diveri ifolia) dalam menurunkan kadar gula darah pada mencit (Mus muscu/us) yang diinduksi dengan aloksan. Penggunaan aloksan dimaksudkan untuk memperoleh gambaran Diabetes Militus (DM) buatan. Pembuktian tentang pengaruh ekstrak batang kayu manis (Cinnamomum burmanii) dan ekstrak daun kembang bulan (7'ithonia diversifilia) dilihat adanya revitalisasi sel beta pankreas

Detection of 37 kDa Outer Membrane Protein H (ompH) gene of Pasteurella multocida Type B Local Isolate From Nusa Temggara Timur (NTT)

Haemorrhagic Septicaemia (HS), in Indonesia known as Ngorok disease is a fatal acute septicemia disease in cattle and buffalo caused by Pasteurella multocida. Omp has a number of important roles for bacterial cells, such as uptake of nutrients, transporter for molecules in and out of cells and interactions with the host and environment. Outer membrane Protein H (OmpH) in P. multocida is one of the major proteins in the envelope which has been purified and characterized as porin immunodominant. The purpose in of this study was to detect the 37d kDa outer membrane protein H (ompH) gene from P. multocida local isolate from Nusa Tenggara Timur (NTT) and compare it with vaccine strain. Detection of 37 kDa ompH of P. multocida was done using PCR. Sample used in this study were P. multocida local isolate from NTT and vaccine strain (Katha). Isolation and identification were performed using bacteriological culture and biochemically characterization. Results from PCR showed a 37 kDa ompH gene on P. multocida local isolate and vaccine strain is 946 b

The Dilatation of Brain Ventricle Due to Congenital Toxoplasmosis in Mice Correlated With Apoptosis But Not With Transforming Growth Factor Beta

This study aimed to determine the occurences of mice brain ventricles dilatation that congenitally infected with Toxoplasma gondii (T. gondii) as a marker of hydrocephalus and cellular changes in the brain. A total of twenty pregnant mice (11.5 days pregnacy) were divided intotwo groups, which were control (P1) group and treatment (P2) group. The mice in the treatment group were infected with 101 tachyzoites of T. gondii. All mice were maintained until delivery. The newborn mice were sacrificed and their brain were removed and fixed in 10% buffered formalin to prepare histology slides with HE staining for observation of ventricular width, TUNEL assay for apoptosis observation, and immunohistochemistry for the expression of transforming growth factor beta (TGF β) observations. The data were analyzed using t test and linear regression. The results showed that ventricular width and apoptosis index significantly increased (P0.05) in both groups. Dilatation of ventricle correlated with the apoptotic index of brain cells but did not correlated with the expression of TGF-β. Key words: apoptotic index, brain ventricle, hydrocephalus, Toxoplasma gondi