692 research outputs found
MEASUREMENT OF CORTISOL AND CORTISONE IN HUMAN SALIVA BY UPLC-MS/MS
Objective: To develop and validate a simple and rapid assay for simultaneous measurement of cortisol and cortisone in human saliva by ultra-performance liquid chromatography-tandem mass spectrometry.
Methods: Chromatographic analysis was performed on an Atlantis dC18 column (2.1 x 100 mm, 3 µm) using a mobile phase consisting of acetonitrile and 2 mmol ammonium-acetate (50:50, v; v) that was delivered at a flow rate of 0.3 ml/min. The eluents were monitored using electrospray ionization in the positive ion mode set at transition set of mass-to-charge (m/z): 363.11 → 121.00, 361.18 → 163.11, and 367.19 → 121.24 for cortisol, cortisone and internal standard (IS), respectively the method was validated for linearity, accuracy, precision, and recovery, according to international guidelines.
Results: The retention times of cortisol, cortisone and internal were about 1.38, 1.43 and 1.38 min, respectively. Cortisol level and cortisone level relationship to the ratio of their respective peak-area to IS’s peak-area was linear (range of 0.5-100 ng/ml). Coefficients of variation and inaccuracy were, ≤9.9% and-0.3 to 6.9 for cortisol and ≤8.4 and-1.5 to 4.8 for cortisone, respectively. Extraction recoveries for cortisol, cortisone, and the IS were 90%, 94%, and 98%, respectively. Cortisol and cortisone stability was evaluated in processed saliva samples (stored at room temperature for 24 h) and unprocessed saliva samples (stored at room temperature for 24 h or at-20 °C for 10 w) and after 3 freeze-thaw cycles was ≥ 86%.
Conclusion: The proposed method is simple, precise, and accurate for the rapid simultaneous measurement of cortisol and cortisone levels in saliva. The assay was successfully applied to determine levels of cortisol and cortisone in human saliva samples obtained from healthy volunteers
Efecto del extracto de cáscara de granada tunecina sobre la estabilidad oxidativa del aceite de maíz en condiciones de calentamiento
The effect of pomegranate peel extract (PPE) on the oxidative stability of corn oil during heating was studied. Oxidation was followed by determining peroxide value (PV), p-anisidine value (p-AV), free fatty acid value (FFA), conjugated dienes (CD), conjugated trienes hydroperoxides (CT) and the calculated total oxidation value (TOTOX). Polyphenol (TPC) and ortho-diphenol (TOPC) contents as well as the antioxidant activity of each oil sample were evaluated before and after heating. PPE showed a significant inhibitory effect on lipid oxidation. Heating samples for 8 hours supplemented by PPE to a level of 1000 ppm resulted in the highest significant decreases in investigated indices compared to the control and BHT values. It was concluded that the antioxidant activity of PPE delayed oxidation and can be used in the food industry to prevent and reduce lipid deterioration in oil.Se estudió el efecto del extracto de cáscara de granada (ECG) sobre la estabilidad oxidativa del aceite de maíz durante condiciones de calentamiento. La oxidación se siguió mediante la determinación del índice de peróxido (IP), el índice de p-anisidina (p-AV), el índice de acidez (IA), los dienos conjugados (DC), los hidroperóxidos de trienos conjugados (TC) y el valor calculado de la oxidación total (TOTOX). Se evaluó el contenido de polifenoles totales (PT) y de orto-difenoles (o-DF), así como la actividad antioxidante de cada muestra de aceite, antes y después del calentamiento. El ECG mostró un efecto inhibidor significativo sobre la oxidación de lípidos. El calentamiento de las muestras, durante 8 horas suplementadas con ECG a un nivel de 1000 ppm, dio como resultado una significativa disminución de los índices investigados en relación con los valores de control y con BHT. Se concluyó que la actividad antioxidante de los ECG retrasó la oxidación y que se puede utilizar en la industria alimentaria para prevenir y reducir el deterioro de los lípidos del aceite
QUANTITATION OF AMLODIPINE IN HUMAN PLASMA BY LCMS/MS ASSAY
ÂÂÂÂÂÂÂÂÂÂÂÂÂÂÂÂÂObjective: To develop and validate a simple, precise, and rapid liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for quantification of amlodipine in human plasma.Methods: Chromatographic analysis was performed on Atlantis dC18 column (2.1 x 100 mm, 3 µm) with a mobile phase consisting of acetonitrile and 10 mM formic acid (80:20, v: v) that was delivered at a flow rate of 0.3 ml/min. The eluents were monitored using electrospray ionization in the positive ion mode set at transition 409 → 238.4 and 254.3 → 43.9 for amlodipine and tizanidine hydrochloride (IS), respectively. The method was validated for linearity, accuracy, precision, and recovery as per US-FDA guidelines.Results: The retention times of amlodipine and tizanidine (IS) were 1.26 and 1.22 respectively. The relationship between amlodipine concentration and peak height ratio of amlodipine to the IS was linear (R2³ 0.9868) in the range of 0.2–20 ng/ml, and the intra-and inter-day coefficient of variations and bias were ≤14.4% and ≤13.6% and ≤13.7% and ≤11.2%, respectively.Conclusion: The proposed method is simple, precise, and accurate for rapid measurement of amlodipine level using 0.5 ml human plasma. Further, the assay was successfully applied to determine amlodipine level in human plasma samples obtained from a healthy volunteer
L'atteinte du sinus caverneux dans le carcinome adenoïde systique
Introduction: Head and neck adenoid cystic carcinoma (ACC) is a malignant epithelial neoplasm, developing from salivary gland tissue. Cavernous sinus and skull base invasion by perinervous spread is a particular behavior of this tumor entity. We report two cases of cavernous sinus invasion.Cases report: The first one was originated in the parotid gland, invaded infratemporal fossa and extended to cavernous sinus through perineural infiltration along trigeminal nerve. Second case of ACC was developed in submandibular gland. An invasion of cavernous sinus was observed two years later despite a surgical resection with post operative chemoradiotherapy.Discussion: ACC is characterized by locally aggressive invasion and a strong tendency to recur. Perineural spread extension is common in ACC, as a precursor of skull base invasion and cavernous sinus involvment along the mandibular, maxillary and facial nerves. Magnetic resonance imaging (MRI) has a higher sensitivity and specificity in detecting perineural spread. Surgical therapy is the choice treatment although complete resection is not possible in all cases due to the infiltration andperineural spread. So, radiation therapy is considered effective, and has been used successfully in ACC invading cavernous sinus. The place of chemotherapy has yet to be determinate. The prognosis of these extensive tumors is bad because high frequency of local recurrence and distant metastases.Key-words: adenoid cystic carcinoma, cavernous sinus, skull base, perineural invasion
Cerebellar liponeurocytoma with extracranial extension: Case report
Cerebellar liponeurocytoma is a newly recognized, rare clinicopathological entity commonly described in the cerebellar hemispheres or the vermis.
We present a rare case of cerebellar liponeurocytoma arising from the left cerebellar amygdala with extracranial extension. Such a condition has never been previously reported
Chemical composition, antibacterial and antioxidant activities of essential oils from flowers, leaves and aerial parts of Tunisian Dittrichia Viscosa
The objective of this work was to determine the chemical composition, the antioxidant and antibacterial activities of the essential oils (EOs) extracted by hydrodistillation from different organs of the D. viscosa: flowers, leaves and aerial parts. The main compounds identified by GC/MS are oxygenated sesquiterpenes. Among these compounds, (E)-nerolidol (40.7%) is the most abundant constituent of flowers’ essential oil while caryophyllene oxide (9.9%), isolongifolan-7-α-ol (10.3%) and α -eudesmol (9.1%) are the major constituents of the leaves’ essential oil. The presence of these compounds in the aerial parts’ essential oil is solely due to those of the flowers and leaves that constitute these aerial parts. The volatile extracts showed antioxidant effects with IC50 values ranging between 9.25 and 9.75 mg.mL−1. On the other hand, EOs showed antibacterial effects on both Gram-positive and Gram-negative bacteria. The highest activity was obtained with flowers’ essential oil against Enterococcus faecalis and Escherichia coli
La tuberculose extra-ganglionnaire de la sphere O.R.L.
La tuberculose extra ganglionnaire est une pathologie rare en ORL. Elle pourrait poser des problèmes diagnostiques surtout avec la pathologie tumorale. Nous rapportons 10 cas de localisations extra-ganglionnaires, colligés au service ORL de l\'hôpital Tahar Sfar de Mahdia entre 1988 et 2004. Il s\'agissait de 7 femmes et 3 hommes, âgés entre 19 et 77 ans (moyenne : 39 ans). La maladie a touché le cavum dans 3 cas, les glandes salivaires dans 3 cas, les amygdales palatines dans 2 cas et le larynx chez 2 patients. Le diagnostic était dans tous les cas histo-pathologique. Tous nos patients ont reçu un traitement anti-tuberculeux. L\'évolution était favorable avec un recul moyen de 11 mois. Conclusion : La tuberculose extra-ganglionnaire en ORL pose surtout des problèmes diagnostiques. Son diagnostic est le plus souvent histo-pathologique. Son traitement repose sur la himiothérapie anti-tuberculeuse. Extra-nodal tuberculosis is a rare in ENT practice. We report 10 cases of extra-ganglionic localizations of tuberculosis, treated in ENT department of the Tahar Sfar hospital of Mahdia between 1988 and 2004. They were 7 women and 3 men, aged between 19 and 77 years (average: 39 years) . The disease has touched the nasopharynx in 3 cases, the salivary glands in 3 cases, the tonsils in 2 cases and the larynx in 2 cases. The diagnosis was in all cases histo-pathological. All our patients received an anti-tubercular treatment. The evolution was favorable with 11 months average outcome. Conclusion: Extra-nodal tuberculosis in ENT poses especially diagnostic problems. Its treatment is based on anti-tubercular chemotherapy. Journal Tunisien d\'ORL et de chirurgie cervico-faciale Vol. 16 2006: pp. 36-3
Isokinetic muscle strengthening after acquired cerebral damage: A literature review
AbstractObjectiveIsokinetic strengthening is a rehabilitation technique rarely used in stroke patients. However, the potential benefits of force and endurance training in this population are strongly suspected.MethodThis literature review synthesizes the results of clinical trials on this topic. The research was conducted on PubMed, using “Stroke”, “rehabilitation”, “isokinetic”, “upper limb” and “training” as keywords.ResultsSeventeen studies focusing on the use of isokinetics in assessment or rehabilitation (six studies) following stroke were reviewed. For the lower limb, muscle strength and walking ability improved after isokinetic rehabilitation programs. For the upper limb, the only two studies found in the literature suggest improvement in the strength of the trained muscles, of grip force, of the Fugl-Meyer motor score and of global functional capacities. This review does not reveal any consensus on the protocols to be implemented: type of muscle contraction, velocities….ConclusionWhile isokinetic strengthening has not proven its efficiency in rehabilitation of the upper limb following stroke, its interest with regard to rehabilitation of the lower limbs has been recognized. Randomized controlled trials in this field are necessary to confirm its efficiency, especially concerning upper arm rehabilitation
Carcinome basocellulaire métatypique : a propos de deux cas
Introduction : : Le carcinome métatypique est une entité rare. Il touche essentiellement la région cervico-faciale chez un sujet de sexe masculin. Les auteurs étudieront, à travers deux observations, les caractéristiques cliniques et évolutives du cancer métatypique ainsi que les difficultés thérapeutiques. Observation 1 : Un homme âgé de 41 ans, a présenté un carcinome métatypique temporo-pariétal droit traité initialement par chirurgie. Cinq ans plus tard, il a été réopéré pour une récidive locale et ganglionnaire et a bénéficié ensuite d’une cobaltothérapie, stabilisant ainsi la maladie pendant 4 ans au bout desquels on a noté la survenue d’une deuxième récidive locale avec des métastases pulmonaires. Le malade est décédé, à 10 ans de recul, dans un tableau de pneumopathie diffuse avec septicémie sévère. Observation 2 : Un homme de 71 ans, a présenté un carcinome métatypique de la région rétro- auriculaire. Il a eu une exérèse tumorale avec des limites chirurgicales envahies. Il a présenté plusieurs récidives locales traitées par des exérèses larges jusqu’à une pétrectomie totale. Les limites chirurgicales étaient toujours tumorales. Une radiothérapie postopératoire a été réalisée pour chaque récidive. A 7 ans de recul, il a présenté une récidive locale envahissant l’angleponto-cérébelleux et le cerveau, jugée en dehors de toutes ressources thérapeutiques. Discussion : Le carcinome métatypique est caractérisé par son agressivité et sa tendance à la récidive. Son traitementest essentiellement chirurgical. Une large marge de sécurité lors de l’exérèse est indispensable. La radiothérapie est un traitement adjuvant, devenu incontournable vu le haut risque de récidive. Le rôle de la chimiothérapie n’est pas encore prouvé.
Traitement des diplegies laryngees en fermeture: Apport du laser
Introduction: Les diplegies laryngees en fermeture sont rares et mal tolerees par les patients. Leur prise en charge therapeutique a connu de nombreuses avancees au cours des dernieres annees. Le but de ce travail est dfetudier la place du laser dans leur prise en charge.Malades et methodes : Notre etude est retrospective a propos de six patients porteurs de diplegie laryngee en fermeture. Lfage moyen etait de 46 ans (16 et 70 ans). Lfetiologie etait une intubation prolongee ou traumatique dans 3 cas, post thyroidectomie totale dans deux autres cas et idiopathique dans 1 cas. Le traitement a consiste en une tracheotomie en urgence dans 5 cas. Tous les patients ont beneficie dans un secondtemps dfun traitement au laser apres 9 mois de surveillance.Resultats : La cordectomie posterieure a ete realisee en premier temps dans 4 cas. Elle etait bilaterale en deux temps dans 2 cas et unilaterale dans 2 cas. Une aryteno.dectomie initiale a ete realisee pour les deux autres patients. Une revision chirurgicale a consiste en une arytenoidectomie dans 2 cas et une cordotomie partielle posterieure controlaterale dans un cas. La decanulation etait bien toleree pour 4 patients. Un seul malade a garde une gene respiratoire sans veritabledyspnee avec une canule parlante fermee et la decanulation a ete refusee. Une voix satisfaisante a ete obtenue pour tous les malades. Des fausses route transitoires ont ete note dans 2 cas.Discussion : Actuellement, le traitement endoscopique au laser constitue le traitement de choix dans la diplegie laryngee et doit etre propose en premiere intension. Deux techniques principales sont decrites : lfaryteno.dectomie au laser type Ossof et la cordectomie posterieure transverse de kashima. plusieurs series ont defendu chacune des deux techniques. Notre serie a montre que les deux techniques peuvent etre associees pour donner de meilleurs resultats.Mots cles : diplegie laryngee, Laser, aryteno.dectomie, cordectomie posterieure
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