21,207 research outputs found
Under-reporting of roadcasualties ? phase 1
Although this report was commissioned by the Department for Transport, the findings and recommendations are those of the authors and do not necessarily represent the views of the DfT
Isolated sequences from the linked Myf-5 and MRF4 genes drive distinct patterns of muscle-specific expression in transgenic mice
In developing mouse embryos, MyoD family regulatory genes are expressed specifically in muscle precursors and mature myofibers. This pattern, taken together with the well-established ability of MyoD family members to convert a variety of cell types to skeletal muscle, suggests a significant role for these genes in regulating skeletal myogenesis. The possibility that expression of these genes may be causally associated with segregation of the myogenic lineage from other mesodermal derivatives, or with the subsequent maintenance of muscle phenotypes at later times, raises the issue of how MyoD family genes are themselves regulated during development. In this work, we have initiated studies to identify DNA sequences that govern Myf-5 and MRF4 (herculin, myf-6) transcription. Myf-5 is the first of the MyoD family to be expressed in the developing mouse embryo, while MRF4 is the most abundantly expressed myogenic factor in postnatal animals. In spite of their strikingly divergent patterns of expression, Myf-5 and MRF4 are tightly linked in the mouse genome; their translational start codons are only 8.5 kilobases apart. Here, the 5' flanking regions of the mouse Myf-5 and MRF4 genes were separately linked to a bacterial β-galactosidase (lacZ) gene, and these constructs were each used to produce several lines of transgenic mice. Transgene expression was monitored by X-gal staining of whole embryos and by in situ hybridization of embryo sections. For the Myf-5/lacZ lines, the most intense transgene expression was in the visceral arches and their craniofacial muscle derivatives, beginning at day 8.75 post coitum (p.c.). This correlates with endogenous Myf-5 expression in visceral arches. However, while Myf-5 is also expressed in somites starting at day 8 p.c., transgene expression in the trunk is not observed until day 12 p.c. Thus, the Myf-5/lacZ construct responds to early Myf-5 activators in the visceral arches but not in the somites, suggesting that myogenic determination in the nonsomitic head mesoderm may be under separate control from that of the somitic trunk mesoderm. MRF4/lacZ lines displayed an entirely different pattern from Myf-5. Transgene expression appeared in muscles starting at day 16.5 p.c. and became increasingly prominent at later times. However, an early wave of myotomal expression that is characteristic of the endogenous MRF4 was not recapitulated by the transgene
Fatal injuries to car occupants: analysis of health and population data
Although this report was commissioned by the Department for Transport (DfT), the findings and recommendations are those of the authors and do not necessarily represent the views of the DfT. While the DfT has made every effort to ensure the information in this document is accurate, DfT does not guarantee the accuracy, completeness or usefulness of that information; and it cannot accept liability for any loss or damages of any kind resulting from reliance on the information or guidance this document contains
Transient Random Walks in Random Environment on a Galton-Watson Tree
We consider a transient random walk in random environment on a
Galton--Watson tree. Under fairly general assumptions, we give a sharp and
explicit criterion for the asymptotic speed to be positive. As a consequence,
situations with zero speed are revealed to occur. In such cases, we prove that
is of order of magnitude , with . We also
show that the linearly edge reinforced random walk on a regular tree always has
a positive asymptotic speed, which improves a recent result of Collevecchio
\cite{Col06}
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Effects of Pre-reading Instructions on the Comprehension of Science Texts
This study examined how three different pre-reading (or relevance) instructions led to different learning outcomes for middle school students reading science texts on the topic of sweetness. The first was a generic instruction to read for understanding. The second prompted students to form a holistic explanation of the topic of sweetness, and the third instruction prompted students to focus on the core scientific principle of the relationship between structure and function. The latter two were specifically designed to align with science disciplinary goals. A comparison of the three treatments found that the generic instruction and the structure-function instruction led to better learning outcomes, measured by recall, short-answer performance questions, and a traditional multiple-choice/short-answer assessment. A qualitative analysis of the data also revealed some small yet notable differences in the recall pattern of students, such as an increased recall of key ideas for the structure-function instruction. This effect was seen predominantly for higher-skilled readers. The results suggest the possibility that relevance instructions targeting core ideas may help to orient students to the key ideas and explanations in scientific text, especially for higher-skilled readers, and indirectly highlights some of the challenges for students with less reading competencies. Overall, this study provides greater insight into how middle-school students read science texts, the effectiveness of instructor-provided relevance instructions in promoting (higher-level) comprehension of science texts, and implications for teachers on how to use texts in science instruction
Inventory Information
In a market with symmetric information about fundamentals, can information-based trade still arise? Consider bond and FX markets, where private information about nominal cash flows is generally absent, but participants are convinced that superior information exists. We analyze a class of asymmetric information - inventory information - that is unrelated to fundamentals, but still forecasts future price (by forecasting future discount factors). Empirical work based on the analysis shows that inventory information in FX does indeed forecast discount factors, and does so over both short and long horizons. The immediate price impact of shocks to inventory information is large, roughly 50 percent of that from public information shocks (the latter being the whole story under symmetric information). Within about 30 minutes the transitory effect dies out, and prices reflect a permanent effect from inventory information that ranges between 15 and 30 percent of that from public information.
Is the Sun Lighter than the Earth? Isotopic CO in the Photosphere, Viewed through the Lens of 3D Spectrum Synthesis
We consider the formation of solar infrared (2-6 micron) rovibrational bands
of carbon monoxide (CO) in CO5BOLD 3D convection models, with the aim to refine
abundances of the heavy isotopes of carbon (13C) and oxygen (18O,17O), to
compare with direct capture measurements of solar wind light ions by the
Genesis Discovery Mission. We find that previous, mainly 1D, analyses were
systematically biased toward lower isotopic ratios (e.g., R23= 12C/13C),
suggesting an isotopically "heavy" Sun contrary to accepted fractionation
processes thought to have operated in the primitive solar nebula. The new 3D
ratios for 13C and 18O are: R23= 91.4 +/- 1.3 (Rsun= 89.2); and R68= 511 +/- 10
(Rsun= 499), where the uncertainties are 1 sigma and "optimistic." We also
obtained R67= 2738 +/- 118 (Rsun= 2632), but we caution that the observed
12C17O features are extremely weak. The new solar ratios for the oxygen
isotopes fall between the terrestrial values and those reported by Genesis
(R68= 530, R6= 2798), although including both within 2 sigma error flags, and
go in the direction favoring recent theories for the oxygen isotope composition
of Ca-Al inclusions (CAI) in primitive meteorites. While not a major focus of
this work, we derive an oxygen abundance of 603 +/- 9 ppm (relative to
hydrogen; 8.78 on the logarithmic H= 12 scale). That the Sun likely is lighter
than the Earth, isotopically speaking, removes the necessity to invoke exotic
fractionation processes during the early construction of the inner solar
system
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